PMID- 25970178
OWN - NLM
STAT- MEDLINE
DCOM- 20160222
LR  - 20240326
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 10
IP  - 5
DP  - 2015
TI  - Origin, divergence, and phylogeny of asexual Epichloe endophyte in Elymus species 
      from western China.
PG  - e0127096
LID - 10.1371/journal.pone.0127096 [doi]
LID - e0127096
AB  - Asexual Epichloe species are likely derived directly from sexual Epichloe species 
      that then lost their capacity for sexual reproduction or lost sexual reproduction 
      because of interspecific hybridization between distinct lineages of sexual 
      Epichloe and/or asexual Epichloe species. In this study we isolated asexual 
      Epichloe endophytes from Elymus species in western China and sequenced 
      intron-rich regions in the genes encoding beta-tubulin (tubB) and translation 
      elongation factor 1-alpha (tefA). Our results showed that there are no gene copies of 
      tubB and tefA in any of the isolates. Phylogenetic analysis showed that sequences 
      in this study formed a single clade with asexual Epichloe bromicola from Hordeum 
      brevisubulatum, which implies asexual Epichloe endophytes that are symbionts in a 
      western Chinese Elymus species likely share a common ancestor with asexual E. 
      bromicola from European H. brevisubulatum. In addition, our results revealed that 
      asexual E. bromicola isolates that are symbionts in a western Chinese Elymus 
      species and sexual Epichloe species that are symbionts in a North American Elymus 
      species have a different origin. Further analysis found that Epichloe species 
      likely originated in Eurasia. In addition, the results support the hypothesis 
      that migratory birds or humans might have aided the dispersal of these fungal 
      endophytes to other continents.
FAU - Song, Hui
AU  - Song H
AD  - Key Laboratory of Grassland Agro-Ecosystems, Lanzhou, 730020, P. R. China; 
      College of Pastoral Agriculture Science and Technology, Lanzhou University, 
      Lanzhou, 730020, P. R. China.
FAU - Nan, Zhibiao
AU  - Nan Z
AD  - Key Laboratory of Grassland Agro-Ecosystems, Lanzhou, 730020, P. R. China; 
      College of Pastoral Agriculture Science and Technology, Lanzhou University, 
      Lanzhou, 730020, P. R. China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150513
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (Tubulin)
SB  - IM
MH  - Ascomycota/classification/*genetics/isolation & purification
MH  - China
MH  - Elymus/*microbiology
MH  - Endophytes/*genetics/isolation & purification
MH  - Genes, Fungal
MH  - Haplotypes
MH  - Models, Genetic
MH  - Molecular Typing
MH  - Mycological Typing Techniques
MH  - Phylogeny
MH  - Plant Dispersal
MH  - Reproduction, Asexual
MH  - Sequence Analysis, DNA
MH  - Tubulin/genetics
PMC - PMC4430518
COIS- Competing Interests: The authors have declared that no competing interests exist.
EDAT- 2015/05/15 06:00
MHDA- 2016/02/24 06:00
PMCR- 2015/05/13
CRDT- 2015/05/14 06:00
PHST- 2014/10/03 00:00 [received]
PHST- 2015/04/10 00:00 [accepted]
PHST- 2015/05/14 06:00 [entrez]
PHST- 2015/05/15 06:00 [pubmed]
PHST- 2016/02/24 06:00 [medline]
PHST- 2015/05/13 00:00 [pmc-release]
AID - PONE-D-14-44374 [pii]
AID - 10.1371/journal.pone.0127096 [doi]
PST - epublish
SO  - PLoS One. 2015 May 13;10(5):e0127096. doi: 10.1371/journal.pone.0127096. 
      eCollection 2015.