PMID- 25971527
OWN - NLM
STAT- MEDLINE
DCOM- 20170208
LR  - 20181202
IS  - 1591-9528 (Electronic)
IS  - 1591-8890 (Linking)
VI  - 16
IP  - 3
DP  - 2016 Aug
TI  - miRNA-202 in bone marrow stromal cells affects the growth and adhesion of 
      multiple myeloma cells by regulating B cell-activating factor.
PG  - 307-16
LID - 10.1007/s10238-015-0355-4 [doi]
AB  - Bone marrow stromal cells (BMSCs) up-regulate B cell-activating factor (BAFF) in 
      multiple myeloma. Increasing experimental evidence has shown that microRNAs play 
      a causal role in hematology tumorigenesis. In this study, we characterized the 
      role of miR-202 in regulating the expression of BAFF in BMSCs. It was found that 
      expressions of BAFF mRNA and protein were increased in BMSCs treated with miR-202 
      inhibitor. The growth rate of miR-202 mimics transfection cells was significantly 
      lower than that of non-transfected cells. The expression of Bcl-2 protein was 
      down-regulated, and Bax protein was up-regulated after miR-202 mimics 
      transfection. Over-expression of miR-202 in BMSCs rendered MM cells more 
      sensitive to bortezomib. More significantly, the regulatory effect of miR-202 
      could inhibit the activation of NF-kappaB pathway in BMSCs. These results suggest 
      that miR-202 functions as a modulator that can negatively regulate BAFF by 
      inhibiting MM cell survival, growth, and adhesion in the bone marrow 
      microenvironment.
FAU - Shen, Xianjuan
AU  - Shen X
AD  - Surgical Comprehensive Laboratory, Affiliated Hospital of Nantong University, #20 
      Xisi Road, Nantong, 226001, JS, People's Republic of China.
FAU - Guo, Yuehua
AU  - Guo Y
AD  - Nantong University, #9 Seyuan Road, Nantong, 226001, JS, People's Republic of 
      China.
FAU - Yu, Jiajia
AU  - Yu J
AD  - The First Hospital Affiliated to Soochow University, #20 Shizi Road, Suzhou, 
      215006, JS, People's Republic of China.
FAU - Qi, Jing
AU  - Qi J
AD  - Surgical Comprehensive Laboratory, Affiliated Hospital of Nantong University, #20 
      Xisi Road, Nantong, 226001, JS, People's Republic of China.
FAU - Shi, Wei
AU  - Shi W
AD  - Surgical Comprehensive Laboratory, Affiliated Hospital of Nantong University, #20 
      Xisi Road, Nantong, 226001, JS, People's Republic of China.
FAU - Wu, Xinhua
AU  - Wu X
AD  - Surgical Comprehensive Laboratory, Affiliated Hospital of Nantong University, #20 
      Xisi Road, Nantong, 226001, JS, People's Republic of China.
FAU - Ni, Hongbing
AU  - Ni H
AD  - Laboratory Medicine Center, Affiliated Hospital of Nantong University, #20 Xisi 
      Road, Nantong, 226001, JS, People's Republic of China.
FAU - Ju, Shaoqing
AU  - Ju S
AD  - Surgical Comprehensive Laboratory, Affiliated Hospital of Nantong University, #20 
      Xisi Road, Nantong, 226001, JS, People's Republic of China. jsq814@hotmail.com.
AD  - Nantong University, #9 Seyuan Road, Nantong, 226001, JS, People's Republic of 
      China. jsq814@hotmail.com.
AD  - Laboratory Medicine Center, Affiliated Hospital of Nantong University, #20 Xisi 
      Road, Nantong, 226001, JS, People's Republic of China. jsq814@hotmail.com.
LA  - eng
PT  - Journal Article
DEP - 20150514
PL  - Italy
TA  - Clin Exp Med
JT  - Clinical and experimental medicine
JID - 100973405
RN  - 0 (B-Cell Activating Factor)
RN  - 0 (BAX protein, human)
RN  - 0 (Cyclins)
RN  - 0 (MIRN202 microRNA, human)
RN  - 0 (MicroRNAs)
RN  - 0 (TNFSF13B protein, human)
RN  - 0 (bcl-2-Associated X Protein)
SB  - IM
MH  - Aged
MH  - B-Cell Activating Factor/*metabolism
MH  - B-Lymphocytes/*physiology
MH  - *Cell Adhesion
MH  - *Cell Proliferation
MH  - Cyclins/analysis
MH  - Female
MH  - Gene Expression Profiling
MH  - Humans
MH  - Male
MH  - Mesenchymal Stem Cells/*metabolism
MH  - MicroRNAs/*metabolism
MH  - Middle Aged
MH  - Multiple Myeloma/*pathology
MH  - bcl-2-Associated X Protein/analysis
OTO - NOTNLM
OT  - B cell-activating factor (BAFF)
OT  - Bone marrow stromal cells (BMSCs)
OT  - Drug resistance
OT  - Multiple myeloma (MM)
OT  - microRNA-202 (miR-202)
EDAT- 2015/05/15 06:00
MHDA- 2017/02/09 06:00
CRDT- 2015/05/15 06:00
PHST- 2014/12/19 00:00 [received]
PHST- 2015/04/24 00:00 [accepted]
PHST- 2015/05/15 06:00 [entrez]
PHST- 2015/05/15 06:00 [pubmed]
PHST- 2017/02/09 06:00 [medline]
AID - 10.1007/s10238-015-0355-4 [pii]
AID - 10.1007/s10238-015-0355-4 [doi]
PST - ppublish
SO  - Clin Exp Med. 2016 Aug;16(3):307-16. doi: 10.1007/s10238-015-0355-4. Epub 2015 
      May 14.