PMID- 25971936
OWN - NLM
STAT- MEDLINE
DCOM- 20160510
LR  - 20181113
IS  - 2160-1836 (Electronic)
IS  - 2160-1836 (Linking)
VI  - 5
IP  - 7
DP  - 2015 May 12
TI  - No Significant Increase in the Delta4- and Delta7-Dafachronic Acid Concentration in the 
      Long-Lived glp-1 Mutant, nor in the Mutants Defective in Dauer Formation.
PG  - 1473-9
LID - 10.1534/g3.115.018812 [doi]
AB  - The steroid hormone dafachronic acid (DA) regulates dauer formation and lifespan 
      in Caenorhabditis elegans by binding to the nuclear receptor DAF-12. However, 
      little is known about how DA concentrations change under various physiologic 
      conditions and about how DA/DAF-12 signaling interacts with other signaling 
      pathways that also regulate dauer formation and lifespan. Using a sensitive 
      bioanalytical method, we quantified the endogenous DA concentrations in a 
      long-lived germline-less glp-1 mutant and in the Dauer formation-defective 
      (Daf-d) mutants daf-12, daf-16, daf-5, and daf-3. We found that the DA 
      concentration in the glp-1 mutant was similar to that in the wild type (WT). This 
      result is contrary to the long-held belief that germline loss-induced longevity 
      involves increased DA production and suggests instead that this type of longevity 
      involves an enhanced response to DA. We also found evidence suggesting that 
      increased DA sensitivity underlies lifespan extension triggered by exogenous DA. 
      At the L2/L3 stage, the DA concentration in a daf-12 null mutant decreased to 22% 
      of the WT level. This finding is consistent with the previously proposed positive 
      feedback regulation between DAF-12 and DA production. Surprisingly, the DA 
      concentrations in the daf-16, daf-5, and daf-3 mutants were only 19-34% of the WT 
      level at the L2/L3 stage, slightly greater than those in the Dauer 
      formation-constitutive (Daf-c) mutants at the pre-dauer stage (4-15% of the WT L2 
      control). Our experimental evidence suggested that the positive feedback between 
      DA and DAF-12 was partially induced in the three Daf-d mutants.
CI  - Copyright (c) 2015 Li et al.
FAU - Li, Tie-Mei
AU  - Li TM
AD  - National Institute of Biological Sciences, Beijing, Beijing 102206, China.
FAU - Liu, Weilong
AU  - Liu W
AD  - National Institute of Biological Sciences, Beijing, Beijing 102206, China.
FAU - Lu, Shan
AU  - Lu S
AD  - National Institute of Biological Sciences, Beijing, Beijing 102206, China.
FAU - Zhang, Yan-Ping
AU  - Zhang YP
AD  - National Institute of Biological Sciences, Beijing, Beijing 102206, China.
FAU - Jia, Le-Mei
AU  - Jia LM
AD  - National Institute of Biological Sciences, Beijing, Beijing 102206, China.
FAU - Chen, Jie
AU  - Chen J
AD  - National Institute of Biological Sciences, Beijing, Beijing 102206, China.
FAU - Li, Xiangke
AU  - Li X
AD  - National Institute of Biological Sciences, Beijing, Beijing 102206, China.
FAU - Lei, Xiaoguang
AU  - Lei X
AD  - National Institute of Biological Sciences, Beijing, Beijing 102206, China Beijing 
      National Laboratory for Molecular Sciences, Key Laboratory of Bioorganic 
      Chemistry and Molecular Engineering of Ministry of Education, Department of 
      Chemical Biology, College of Chemistry and Molecular Engineering, Synthetic and 
      Functional Biomolecules Center, and Peking-Tsinghua Center for Life Sciences, 
      Peking University, Beijing 100871, China.
FAU - Dong, Meng-Qiu
AU  - Dong MQ
AUID- ORCID: 0000-0002-6094-1182
AD  - National Institute of Biological Sciences, Beijing, Beijing 102206, China 
      dongmengqiu@nibs.ac.cn.
LA  - eng
GR  - P40 OD010440/OD/NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20150512
PL  - England
TA  - G3 (Bethesda)
JT  - G3 (Bethesda, Md.)
JID - 101566598
RN  - 0 (Caenorhabditis elegans Proteins)
RN  - 0 (Cholestenes)
RN  - 0 (Glp-1 protein, C elegans)
RN  - 0 (Receptors, Notch)
RN  - 0 (delta4-dafachronic acid)
RN  - 0 (delta7-dafachronic acid)
SB  - IM
MH  - Animals
MH  - Animals, Genetically Modified/genetics/metabolism
MH  - Caenorhabditis elegans/genetics/growth & development/*metabolism
MH  - Caenorhabditis elegans Proteins/*genetics/metabolism
MH  - Cholestenes/*analysis
MH  - Chromatography, High Pressure Liquid
MH  - Germ Cells/metabolism
MH  - Larva/metabolism
MH  - Longevity
MH  - Mass Spectrometry
MH  - Mutation
MH  - Real-Time Polymerase Chain Reaction
MH  - Receptors, Notch/*genetics/metabolism
MH  - Signal Transduction
MH  - Temperature
PMC - PMC4502381
OTO - NOTNLM
OT  - C. elegans dauer
OT  - daf-12
OT  - dafachronic acid
OT  - glp-1
OT  - steroid hormone
EDAT- 2015/05/15 06:00
MHDA- 2016/05/11 06:00
PMCR- 2015/05/12
CRDT- 2015/05/15 06:00
PHST- 2015/05/15 06:00 [entrez]
PHST- 2015/05/15 06:00 [pubmed]
PHST- 2016/05/11 06:00 [medline]
PHST- 2015/05/12 00:00 [pmc-release]
AID - g3.115.018812 [pii]
AID - GGG_018812 [pii]
AID - 10.1534/g3.115.018812 [doi]
PST - epublish
SO  - G3 (Bethesda). 2015 May 12;5(7):1473-9. doi: 10.1534/g3.115.018812.