PMID- 25971950 OWN - NLM STAT- MEDLINE DCOM- 20150915 LR - 20220310 IS - 0032-5791 (Print) IS - 0032-5791 (Linking) VI - 94 IP - 7 DP - 2015 Jul TI - A survey of Mycoplasma gallisepticum and Mycoplasma synovaie with avian influenza H9 subtype in meat-type chicken in Jordan between 2011-2015. PG - 1499-503 LID - 10.3382/ps/pev119 [doi] AB - Commercial chickens in Jordan suffer from respiratory disease of undetermined etiology. This study was designed to document the involvement of avian influenza virus (AIV) H9 subtype, Mycoplasma gallisepticum (MG), and Mycoplasma synoviae (MS) in this respiratory disease. In this study, trachea swabs from 350 commercial broiler chicken flocks that suffered from respiratory disease were tested for AIV H9 subtype by using reverse transcription (RT)-PCR and for MG and MS by using PCR. PCR and RT-PCR results showed that 23.7, 8.9, and 6.6% of these flocks were infected with AIV H9 subtype, MS, and MG, respectively, whereas 12.9 and 5.7% of these flocks were infected with both AIV H9 subtype and MS and AIV H9 subtype and MS, respectively. Furthermore, 42.3% of these flocks were negative for the above mentioned respiratory diseases. Further epidemiological studies are recommended to determine risk factors and evaluate the economic consequences of AIV H9 subtype, MG, and MS infections in the region. Furthermore, studies are required to isolate AIV H9 subtype, MG, and MS and develop vaccines against the local field isolates. CI - (c) 2015 Poultry Science Association Inc. FAU - Roussan, Dergham Ahmad AU - Roussan DA AD - Provimi (a Cargill Company), PO Box 499, Amman 11118, Jordan dirgam_al_roussan@cargill.com. FAU - Khawaldeh, Ghassan AU - Khawaldeh G AD - Provimi (a Cargill Company), PO Box 499, Amman 11118, Jordan. FAU - Shaheen, Ibrahim Ali AU - Shaheen IA AD - Provimi (a Cargill Company), PO Box 499, Amman 11118, Jordan. LA - eng PT - Journal Article DEP - 20150512 PL - England TA - Poult Sci JT - Poultry science JID - 0401150 SB - IM MH - Animals MH - *Chickens MH - Disease Outbreaks/*veterinary MH - Influenza A virus/isolation & purification MH - Influenza in Birds/*epidemiology/virology MH - Jordan/epidemiology MH - Mycoplasma Infections/epidemiology/microbiology/*veterinary MH - Mycoplasma gallisepticum/isolation & purification MH - Mycoplasma synoviae/isolation & purification MH - Poultry Diseases/*epidemiology/microbiology/virology MH - Prevalence MH - Reverse Transcriptase Polymerase Chain Reaction/veterinary MH - Seasons MH - Trachea/virology OTO - NOTNLM OT - Avian influenza OT - Jordan OT - Mycoplasma gallisepticum OT - Mycoplasma synoviae OT - broiler EDAT- 2015/05/15 06:00 MHDA- 2015/09/16 06:00 CRDT- 2015/05/15 06:00 PHST- 2015/03/10 00:00 [accepted] PHST- 2015/05/15 06:00 [entrez] PHST- 2015/05/15 06:00 [pubmed] PHST- 2015/09/16 06:00 [medline] AID - S0032-5791(19)32512-X [pii] AID - 10.3382/ps/pev119 [doi] PST - ppublish SO - Poult Sci. 2015 Jul;94(7):1499-503. doi: 10.3382/ps/pev119. Epub 2015 May 12.