PMID- 25972330
OWN - NLM
STAT- MEDLINE
DCOM- 20150728
LR  - 20220316
IS  - 1943-7722 (Electronic)
IS  - 0002-9173 (Linking)
VI  - 143
IP  - 6
DP  - 2015 Jun
TI  - Conventional karyotyping and fluorescence in situ hybridization: an effective 
      utilization strategy in diagnostic adult acute myeloid leukemia.
PG  - 873-8
LID - 10.1309/AJCPP6LVMQG4LNCK [doi]
AB  - OBJECTIVES: Cytogenetics defines disease entities and predicts prognosis in acute 
      myeloid leukemia (AML). Conventional karyotyping provides a comprehensive view of 
      the genome, while fluorescence in situ hybridization (FISH) detects targeted 
      abnormalities. The aim of this study was to compare the utility of karyotyping 
      and FISH in adult AML. METHODS: We studied 250 adult AML cases with concurrent 
      karyotyping and FISH testing. Karyotyping was considered adequate when 20 or more 
      metaphases were analyzed. RESULTS: In total, 220 cases had adequate karyotyping 
      and were classified as normal karyotype/normal FISH (n = 92), normal 
      karyotype/abnormal FISH (n = 4), abnormal karyotype/normal FISH (n = 8), and 
      abnormal karyotype/abnormal FISH (n = 116). The overall karyotype/FISH 
      concordance rate was 97.7% with five discordant cases identified, four from the 
      normal karyotype/abnormal FISH group and one from the abnormal karyotype/abnormal 
      FISH group. No karyotype/FISH discordance was seen in the abnormal 
      karyotype/normal FISH group for the FISH probes evaluated. FISH lent prognostic 
      information in one (0.5%) of 220 cases with normal karyotype/abnormal FISH: 
      CBFB-MYH11 fusion, indicating favorable prognosis. CONCLUSIONS: In adult AML, 
      FISH rarely provides additional information when karyotyping is adequate. We 
      therefore propose an evidence-based, cost-effective algorithmic approach for 
      routine conventional karyotype and FISH testing in adult AML workup.
CI  - Copyright(c) by the American Society for Clinical Pathology.
FAU - He, Rong
AU  - He R
AD  - From the Divisions of Hematopathology and He.Rong@mayo.edu.
FAU - Wiktor, Anne E
AU  - Wiktor AE
AD  - Laboratory Genetics, Department of Laboratory Medicine and Pathology, and.
FAU - Hanson, Curtis A
AU  - Hanson CA
AD  - From the Divisions of Hematopathology and.
FAU - Ketterling, Rhett P
AU  - Ketterling RP
AD  - Laboratory Genetics, Department of Laboratory Medicine and Pathology, and.
FAU - Kurtin, Paul J
AU  - Kurtin PJ
AD  - From the Divisions of Hematopathology and.
FAU - Van Dyke, Daniel L
AU  - Van Dyke DL
AD  - Laboratory Genetics, Department of Laboratory Medicine and Pathology, and.
FAU - Litzow, Mark R
AU  - Litzow MR
AD  - Division of Hematology, Department of Medicine, Mayo Clinic College of Medicine, 
      Rochester, MN.
FAU - Howard, Matthew T
AU  - Howard MT
FAU - Reichard, Kaaren K
AU  - Reichard KK
AD  - From the Divisions of Hematopathology and.
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - England
TA  - Am J Clin Pathol
JT  - American journal of clinical pathology
JID - 0370470
SB  - IM
EIN - Am J Clin Pathol. 2015 Jul;144(1):175. Howard, Matthew H [corrected to Howard, 
      Matthew T]. PMID: 26071478
CIN - Am J Clin Pathol. 2016 Mar;145(3):430-2. PMID: 27124927
MH  - Adult
MH  - Algorithms
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Karyotyping/*methods
MH  - Leukemia, Myeloid, Acute/*genetics
MH  - Male
OTO - NOTNLM
OT  - Genetics
OT  - Hematology
OT  - Hematopathology
OT  - Molecular diagnostics
EDAT- 2015/05/15 06:00
MHDA- 2015/07/29 06:00
CRDT- 2015/05/15 06:00
PHST- 2015/05/15 06:00 [entrez]
PHST- 2015/05/15 06:00 [pubmed]
PHST- 2015/07/29 06:00 [medline]
AID - 143/6/873 [pii]
AID - 10.1309/AJCPP6LVMQG4LNCK [doi]
PST - ppublish
SO  - Am J Clin Pathol. 2015 Jun;143(6):873-8. doi: 10.1309/AJCPP6LVMQG4LNCK.