PMID- 2597277
OWN - NLM
STAT- MEDLINE
DCOM- 19890403
LR  - 20190621
IS  - 0014-5793 (Print)
IS  - 0014-5793 (Linking)
VI  - 243
IP  - 2
DP  - 1989 Jan 30
TI  - 1,2-Diacylglycerol accumulation in human neutrophils does not correlate with 
      respiratory burst activation.
PG  - 399-403
AB  - Measurements of the level of 1,2-diacylglycerol (1,2-DG) during activation of the 
      respiratory burst of human neutrophils by formyl-methionyl-leucyl-phenylalanine 
      (fMLP) in the presence of platelet-activating factor (PAF) or by opsonized 
      particles show that a correlation between accumulation of 1,2-DG and O2 
      consumption does not exist. Inhibition of protein kinase C activity with 
      staurosporine before addition of opsonized particles demonstrates that the first 
      phase of the respiratory burst is not inhibited, whereas the second phase, which 
      is accompanied by a rise in the content of 1,2-DG, is strongly inhibited. This 
      study indicates that accumulation of 1,2-DG cannot be the sole signal for the 
      initiation of the respiratory burst in human neutrophils.
FAU - Koenderman, L
AU  - Koenderman L
AD  - Central Laboratory of the Netherlands Red Cross Blood Transfusion Service, 
      Amsterdam, The Netherlands.
FAU - Tool, A
AU  - Tool A
FAU - Roos, D
AU  - Roos D
FAU - Verhoeven, A J
AU  - Verhoeven AJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - FEBS Lett
JT  - FEBS letters
JID - 0155157
RN  - 0 (1,2-diacylglycerol)
RN  - 0 (Alkaloids)
RN  - 0 (Diglycerides)
RN  - 0 (Glycerides)
RN  - 0 (Platelet Activating Factor)
RN  - 59880-97-6 (N-Formylmethionine Leucyl-Phenylalanine)
RN  - EC 2.7.11.13 (Protein Kinase C)
RN  - H88EPA0A3N (Staurosporine)
SB  - IM
MH  - Alkaloids/pharmacology
MH  - Diglycerides/*blood
MH  - Glycerides/*blood
MH  - Humans
MH  - In Vitro Techniques
MH  - N-Formylmethionine Leucyl-Phenylalanine/pharmacology
MH  - Neutrophils/*metabolism
MH  - *Oxygen Consumption/drug effects
MH  - Phagocytosis
MH  - Platelet Activating Factor/pharmacology
MH  - Protein Kinase C/antagonists & inhibitors
MH  - Staurosporine
EDAT- 1989/01/30 00:00
MHDA- 1989/01/30 00:01
CRDT- 1989/01/30 00:00
PHST- 1989/01/30 00:00 [pubmed]
PHST- 1989/01/30 00:01 [medline]
PHST- 1989/01/30 00:00 [entrez]
AID - 0014-5793(89)80170-X [pii]
AID - 10.1016/0014-5793(89)80170-x [doi]
PST - ppublish
SO  - FEBS Lett. 1989 Jan 30;243(2):399-403. doi: 10.1016/0014-5793(89)80170-x.