PMID- 25973637
OWN - NLM
STAT- MEDLINE
DCOM- 20160212
LR  - 20181113
IS  - 1520-6882 (Electronic)
IS  - 0003-2700 (Print)
IS  - 0003-2700 (Linking)
VI  - 87
IP  - 12
DP  - 2015 Jun 16
TI  - Polymer Coatings in 3D-Printed Fluidic Device Channels for Improved Cellular 
      Adherence Prior to Electrical Lysis.
PG  - 6335-41
LID - 10.1021/acs.analchem.5b01202 [doi]
AB  - This paper describes the design and fabrication of a polyjet-based 
      three-dimensional (3D)-printed fluidic device where poly(dimethylsiloxane) (PDMS) 
      or polystyrene (PS) were used to coat the sides of a fluidic channel within the 
      device to promote adhesion of an immobilized cell layer. The device was designed 
      using computer-aided design software and converted into an .STL file prior to 
      printing. The rigid, transparent material used in the printing process provides 
      an optically transparent path to visualize endothelial cell adherence and 
      supports integration of removable electrodes for electrical cell lysis in a 
      specified portion of the channel (1 mm width x 0.8 mm height x 2 mm length). 
      Through manipulation of channel geometry, a low-voltage power source (500 V max) 
      was used to selectively lyse adhered endothelial cells in a tapered region of the 
      channel. Cell viability was maintained on the device over a 5 day period (98% 
      viable), though cell coverage decreased after day 4 with static media delivery. 
      Optimal lysis potentials were obtained for the two fabricated device geometries, 
      and selective cell clearance was achieved with cell lysis efficiencies of 94 and 
      96%. The bottleneck of unknown surface properties from proprietary resin use in 
      fabricating 3D-printed materials is overcome through techniques to incorporate 
      PDMS and PS.
FAU - Gross, Bethany C
AU  - Gross BC
AD  - Department of Chemistry, Michigan State University, East Lansing, Michigan 48823, 
      United States.
FAU - Anderson, Kari B
AU  - Anderson KB
AD  - Department of Chemistry, Michigan State University, East Lansing, Michigan 48823, 
      United States.
FAU - Meisel, Jayda E
AU  - Meisel JE
AD  - Department of Chemistry, Michigan State University, East Lansing, Michigan 48823, 
      United States.
FAU - McNitt, Megan I
AU  - McNitt MI
AD  - Department of Chemistry, Michigan State University, East Lansing, Michigan 48823, 
      United States.
FAU - Spence, Dana M
AU  - Spence DM
AD  - Department of Chemistry, Michigan State University, East Lansing, Michigan 48823, 
      United States.
LA  - eng
GR  - R01 GM110406/GM/NIGMS NIH HHS/United States
GR  - R21 EB016379/EB/NIBIB NIH HHS/United States
GR  - 1R01GM110406/GM/NIGMS NIH HHS/United States
GR  - 1R21EB016379/EB/NIBIB NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20150529
PL  - United States
TA  - Anal Chem
JT  - Analytical chemistry
JID - 0370536
RN  - 0 (Cell Extracts)
RN  - 0 (Coated Materials, Biocompatible)
RN  - 0 (Dimethylpolysiloxanes)
RN  - 0 (Polystyrenes)
RN  - 63148-62-9 (baysilon)
SB  - IM
MH  - Cell Adhesion
MH  - Cell Extracts/isolation & purification
MH  - Cell Survival
MH  - Coated Materials, Biocompatible/*chemistry
MH  - Dimethylpolysiloxanes/*chemistry
MH  - Electrodes
MH  - Endothelial Cells/cytology
MH  - Equipment Design
MH  - Humans
MH  - *Microfluidic Analytical Techniques/instrumentation
MH  - Polystyrenes/*chemistry
MH  - *Printing, Three-Dimensional/instrumentation
PMC - PMC5362108
MID - NIHMS760480
COIS- Notes The authors declare no competing financial interest.
EDAT- 2015/05/15 06:00
MHDA- 2016/02/13 06:00
PMCR- 2017/03/22
CRDT- 2015/05/15 06:00
PHST- 2015/05/15 06:00 [entrez]
PHST- 2015/05/15 06:00 [pubmed]
PHST- 2016/02/13 06:00 [medline]
PHST- 2017/03/22 00:00 [pmc-release]
AID - 10.1021/acs.analchem.5b01202 [doi]
PST - ppublish
SO  - Anal Chem. 2015 Jun 16;87(12):6335-41. doi: 10.1021/acs.analchem.5b01202. Epub 
      2015 May 29.