PMID- 25975200
OWN - NLM
STAT- MEDLINE
DCOM- 20160622
LR  - 20181202
IS  - 1708-0428 (Electronic)
IS  - 0960-8923 (Print)
IS  - 0960-8923 (Linking)
VI  - 25
IP  - 12
DP  - 2015 Dec
TI  - Roux-en-Y Gastric Bypass Acutely Decreases Protein Carbonylation and Increases 
      Expression of Mitochondrial Biogenesis Genes in Subcutaneous Adipose Tissue.
PG  - 2376-85
LID - 10.1007/s11695-015-1708-5 [doi]
AB  - BACKGROUND: Mitochondrial dysfunction in adipose tissue has been implicated as a 
      pathogenic step in the development of type 2 diabetes mellitus (T2DM). In adipose 
      tissue, chronic nutrient overload results in mitochondria driven increased 
      reactive oxygen species (ROS) leading to carbonylation of proteins that impair 
      mitochondrial function and downregulation of key genes linked to mitochondrial 
      biogenesis. In patients with T2DM, Roux-en-Y gastric bypass (RYGB) surgery leads 
      to improvements in glycemic profile prior to significant weight loss. 
      Consequently, we hypothesized that improved glycemia early after RYGB would be 
      paralleled by decreased protein carbonylation and increased expression of genes 
      related to mitochondrial biogenesis in adipose tissue. METHODS: To evaluate this 
      hypothesis, 16 obese individuals were studied before and 7-8 days following RYGB 
      and adjustable gastric banding (AGB). Subcutaneous adipose tissue was obtained 
      pre- and post-bariatric surgery as well as from eight healthy, non-obese 
      individual controls. RESULTS: Prior to surgery, adipose tissue expression of 
      PGC1alpha, NRF1, Cyt C, and eNOS (but not Tfam) showed significantly lower expression 
      in the obese bariatric surgery group when compared to lean controls (p < 0.05). 
      Following RYGB, but not after AGB, patients showed significant decrease in 
      HOMA-IR, reduction in adipose protein carbonylation, and increased expression of 
      genes linked to mitochondrial biogenesis. CONCLUSIONS: These results suggest that 
      rapid reduction in protein carbonylation and increased mitochondrial biogenesis 
      may explain postoperative metabolic improvements following RYGB.
FAU - Jahansouz, Cyrus
AU  - Jahansouz C
AD  - Department of Surgery, University of Minnesota, 420 Delaware St. SE, MMC 195, 
      Minneapolis, MN, 55455, USA.
FAU - Serrot, Federico J
AU  - Serrot FJ
AD  - Department of Surgery, University of Minnesota, 420 Delaware St. SE, MMC 195, 
      Minneapolis, MN, 55455, USA.
FAU - Frohnert, Brigitte I
AU  - Frohnert BI
AD  - Division of Pediatric Endocrinology, Department of Pediatrics, University of 
      Minnesota, 420 Delaware St. SE, MMC 195, Minneapolis, MN, 55455, USA.
FAU - Foncea, Rocio E
AU  - Foncea RE
AD  - Department of Biochemistry, Molecular Biology and Biophysics, University of 
      Minnesota, 420 Delaware St. SE, MMC 195, Minneapolis, MN, 55455, USA.
FAU - Dorman, Robert B
AU  - Dorman RB
AD  - Department of Surgery, University of Minnesota, 420 Delaware St. SE, MMC 195, 
      Minneapolis, MN, 55455, USA.
FAU - Slusarek, Bridget
AU  - Slusarek B
AD  - Department of Surgery, University of Minnesota, 420 Delaware St. SE, MMC 195, 
      Minneapolis, MN, 55455, USA.
FAU - Leslie, Daniel B
AU  - Leslie DB
AD  - Department of Surgery, University of Minnesota, 420 Delaware St. SE, MMC 195, 
      Minneapolis, MN, 55455, USA.
FAU - Bernlohr, David A
AU  - Bernlohr DA
AD  - Department of Biochemistry, Molecular Biology and Biophysics, University of 
      Minnesota, 420 Delaware St. SE, MMC 195, Minneapolis, MN, 55455, USA.
FAU - Ikramuddin, Sayeed
AU  - Ikramuddin S
AD  - Department of Surgery, University of Minnesota, 420 Delaware St. SE, MMC 195, 
      Minneapolis, MN, 55455, USA. ikram001@umn.edu.
LA  - eng
GR  - R01 DK053189/DK/NIDDK NIH HHS/United States
GR  - R56 DK084669/DK/NIDDK NIH HHS/United States
GR  - P30 DK050456/DK/NIDDK NIH HHS/United States
GR  - DK084669/DK/NIDDK NIH HHS/United States
GR  - DK050456/DK/NIDDK NIH HHS/United States
GR  - R01 DK084669/DK/NIDDK NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PL  - United States
TA  - Obes Surg
JT  - Obesity surgery
JID - 9106714
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Mitochondrial Proteins)
RN  - 0 (NRF1 protein, human)
RN  - 0 (Nuclear Respiratory Factor 1)
RN  - 0 (PPARGC1A protein, human)
RN  - 0 (Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha)
RN  - 0 (RNA, Messenger)
RN  - 0 (TFAM protein, human)
RN  - 0 (Transcription Factors)
RN  - EC 1.14.13.39 (Nitric Oxide Synthase)
RN  - EC 1.9.3.1 (Electron Transport Complex IV)
SB  - IM
MH  - Adult
MH  - Case-Control Studies
MH  - DNA-Binding Proteins/genetics/metabolism
MH  - Diabetes Mellitus, Type 2/metabolism
MH  - Electron Transport Complex IV/genetics/metabolism
MH  - Female
MH  - *Gastric Bypass
MH  - Humans
MH  - Male
MH  - Middle Aged
MH  - Mitochondrial Proteins/genetics/metabolism
MH  - Nitric Oxide Synthase/genetics/metabolism
MH  - Nuclear Respiratory Factor 1/genetics/metabolism
MH  - *Organelle Biogenesis
MH  - Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha
MH  - *Protein Carbonylation
MH  - RNA, Messenger/metabolism
MH  - Subcutaneous Fat/*metabolism
MH  - Transcription Factors/genetics/metabolism
PMC - PMC4648364
MID - NIHMS734544
OTO - NOTNLM
OT  - Adipose tissue
OT  - Adjustable gastric banding
OT  - Mitochondrial biogenesis
OT  - Protein carbonylation
OT  - Roux-en-Y gastric bypass
EDAT- 2015/05/16 06:00
MHDA- 2016/06/23 06:00
PMCR- 2015/12/01
CRDT- 2015/05/16 06:00
PHST- 2015/05/16 06:00 [entrez]
PHST- 2015/05/16 06:00 [pubmed]
PHST- 2016/06/23 06:00 [medline]
PHST- 2015/12/01 00:00 [pmc-release]
AID - 10.1007/s11695-015-1708-5 [pii]
AID - 10.1007/s11695-015-1708-5 [doi]
PST - ppublish
SO  - Obes Surg. 2015 Dec;25(12):2376-85. doi: 10.1007/s11695-015-1708-5.