PMID- 25975747
OWN - NLM
STAT- MEDLINE
DCOM- 20160304
LR  - 20190402
IS  - 1791-3004 (Electronic)
IS  - 1791-2997 (Print)
IS  - 1791-2997 (Linking)
VI  - 12
IP  - 2
DP  - 2015 Aug
TI  - Identification of key transcription factors in caerulein-induced pancreatitis 
      through expression profiling data.
PG  - 2570-6
LID - 10.3892/mmr.2015.3773 [doi]
AB  - The current study aimed to isolate key transcription factors (TFs) in 
      caerulein-induced pancreatitis, and to identify the difference between wild type 
      and Mist1 knockout (KO) mice, in order to elucidate the contribution of Mist1 to 
      pancreatitis. The gene profile of GSE3644 was downloaded from the Gene Expression 
      Omnibus database then analyzed using the t-test. The isolated differentially 
      expressed genes (DEGs) were mapped into a transcriptional regulatory network 
      derived from the Integrated Transcription Factor Platform database and in the 
      network, the interaction pairs involving at least one DEG were screened. Fisher's 
      exact test was used to analyze the functional enrichment of the target genes. A 
      total of 1,555 and 3,057 DEGs were identified in the wild type and Mist1KO mice 
      treated with caerulein, respectively. DEGs screened in Mist1KO mice were 
      predominantly enriched in apoptosis, mitogen-activated protein kinase signaling 
      and other cancer-associated pathways. A total of 188 and 51 TFs associated with 
      pathopoiesis were isolated in Mist1KO and wild type mice, respectively. Out of 
      the top 10 TFs (ranked by P-value), 7 TFs, including S-phase kinase-associated 
      protein 2 (Skp2); minichromosome maintenance complex component 3 (Mcm3); cell 
      division cycle 6 (Cdc6); cyclin B1 (Ccnb1); mutS homolog 6 (Msh6); cyclin A2 
      (Ccna2); and cyclin B2 (Ccnb2), were expressed in the two types of mouse. These 
      TFs were predominantly involved in phosphorylation, DNA replication, cell 
      division and DNA mismatch repair. In addition, specific TFs, including 
      minichromosome maintenance complex component 7 (Mcm7); lymphoid-specific helicase 
      (Hells); and minichromosome maintenance complex component 6 (Mcm6), that function 
      in the unwinding of DNA were identified to participate in Mist1KO pancreatitis. 
      The DEGs, including Cdc6, Mcm6, Msh6 and Wdr1 are closely associated with the 
      regulation of caerulein-induced pancreatitis. Furthermore, other identified TFs 
      were also involved in this type of regulation.
FAU - Qi, Dachuan
AU  - Qi D
AD  - Department of Surgery, The First Affiliated Hospital of Soochow University, 
      Suzhou, Jiangsu 215006, P.R. China.
FAU - Wu, Bo
AU  - Wu B
AD  - Department of Surgery, Shanghai Jiaotong University Affiliated Sixth People's 
      Hospital, Shanghai 200233, P.R. China.
FAU - Tong, Danian
AU  - Tong D
AD  - Department of Surgery, Shanghai Jiaotong University Affiliated Sixth People's 
      Hospital, Shanghai 200233, P.R. China.
FAU - Pan, Ye
AU  - Pan Y
AD  - Department of Surgery, Shanghai Jiaotong University Affiliated Sixth People's 
      Hospital, Shanghai 200233, P.R. China.
FAU - Chen, Wei
AU  - Chen W
AD  - Department of Surgery, Shanghai Jiaotong University Affiliated Sixth People's 
      Hospital, Shanghai 200233, P.R. China.
LA  - eng
PT  - Journal Article
DEP - 20150512
PL  - Greece
TA  - Mol Med Rep
JT  - Molecular medicine reports
JID - 101475259
RN  - 0 (Basic Helix-Loop-Helix Transcription Factors)
RN  - 0 (Bhlha15 protein, mouse)
RN  - 0 (Transcription Factors)
RN  - 888Y08971B (Ceruletide)
SB  - IM
MH  - Animals
MH  - Apoptosis/genetics
MH  - Basic Helix-Loop-Helix Transcription Factors/deficiency/*genetics
MH  - Cell Division
MH  - Ceruletide
MH  - DNA Mismatch Repair
MH  - DNA Replication
MH  - Databases, Genetic
MH  - Gene Expression Profiling
MH  - Gene Expression Regulation
MH  - *Gene Regulatory Networks
MH  - Mice
MH  - Mice, Knockout
MH  - Molecular Sequence Annotation
MH  - Pancreatitis/chemically induced/*genetics/metabolism/pathology
MH  - Signal Transduction
MH  - Transcription Factors/*genetics/metabolism
PMC - PMC4464163
EDAT- 2015/05/16 06:00
MHDA- 2016/03/05 06:00
PMCR- 2015/05/12
CRDT- 2015/05/16 06:00
PHST- 2014/02/14 00:00 [received]
PHST- 2014/11/07 00:00 [accepted]
PHST- 2015/05/16 06:00 [entrez]
PHST- 2015/05/16 06:00 [pubmed]
PHST- 2016/03/05 06:00 [medline]
PHST- 2015/05/12 00:00 [pmc-release]
AID - mmr-12-02-2570 [pii]
AID - 10.3892/mmr.2015.3773 [doi]
PST - ppublish
SO  - Mol Med Rep. 2015 Aug;12(2):2570-6. doi: 10.3892/mmr.2015.3773. Epub 2015 May 12.