PMID- 25976739
OWN - NLM
STAT- MEDLINE
DCOM- 20160525
LR  - 20150814
IS  - 1095-8355 (Electronic)
IS  - 1065-6995 (Linking)
VI  - 39
IP  - 9
DP  - 2015 Sep
TI  - In vitro generation of functional dendritic cells differentiated from CD34 
      negative cells isolated from human umbilical cord blood.
PG  - 1080-6
LID - 10.1002/cbin.10490 [doi]
AB  - Dendritic cells (DCs) are the most potent antigen-presenting cells that play a 
      crucial role in the initiation of an immune response. As DC-based therapeutic 
      applications is increasing, large-scale DC production is required for 
      transplantation. Human umbilical cord blood (UCB) has been shown to contain a 
      rare and precious population of hematopoietic stem cells (HSCs), which can give 
      rise to DCs. The CD34 antigen has been widely used as a cell surface marker to 
      identify HSCs. In this study, we used CD34 antibody to isolate CD34(+) and 
      CD34(-) cells and compared the ability to differentiate into DCs. We used a 
      two-step method combined with the magnetic bead sorting system to isolate CD34(+) 
      and CD34(-) cells from human UCB. Analysis of cellular properties and 
      functionality using a migration assay and T cell proliferation assay revealed no 
      significant differences between CD34(+) cells and CD34(-) cells in their ability 
      to generate DCs.
CI  - (c) 2015 International Federation for Cell Biology.
FAU - Park, Yo Seph
AU  - Park YS
AD  - Department of Stem Cell Biology, School of Medicine, Konkuk University, Seoul, 
      143-701, Korea.
AD  - Center for Stem Cell Research, Institute of Advanced Biomedical Science, Konkuk 
      University, Seoul, 143-701, Korea.
FAU - Shin, Changsik
AU  - Shin C
AD  - School of Life Sciences, Ulsan National Institute of Science and Technology 
      (UNIST), UNIST-gil, 50, Ulsan 689-798, Korea.
FAU - Hwang, Han Sung
AU  - Hwang HS
AD  - Department of Obstetrics and Gynecology, Konkuk University School of Medicine, 
      Seoul 143-729, Korea.
FAU - Zenke, Martin
AU  - Zenke M
AD  - Institute for Biomedical Engineering, Department of Cell Biology, RWTH Aachen 
      University Medical School, Aachen University, Aachen, Germany.
FAU - Han, Dong Wook
AU  - Han DW
AD  - Department of Stem Cell Biology, School of Medicine, Konkuk University, Seoul, 
      143-701, Korea.
FAU - Kang, Young Sun
AU  - Kang YS
AD  - Department of Biomedical Science and Technology, Institute of Advanced Biomedical 
      Science, Konkuk University, Seoul, 143-701, Korea.
FAU - Ko, Kisung
AU  - Ko K
AD  - Department of Medicine, College of Medicine, Chung-Ang University, Seoul, 
      156-756, Korea.
FAU - Do, Yoonkyung
AU  - Do Y
AD  - School of Life Sciences, Ulsan National Institute of Science and Technology 
      (UNIST), UNIST-gil, 50, Ulsan 689-798, Korea.
FAU - Ko, Kinarm
AU  - Ko K
AD  - Department of Stem Cell Biology, School of Medicine, Konkuk University, Seoul, 
      143-701, Korea.
AD  - Center for Stem Cell Research, Institute of Advanced Biomedical Science, Konkuk 
      University, Seoul, 143-701, Korea.
AD  - Research Institute of Medical Science, Konkuk University, Seoul, 143-701, Korea.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150724
PL  - England
TA  - Cell Biol Int
JT  - Cell biology international
JID - 9307129
RN  - 0 (Antigens, CD34)
SB  - IM
MH  - Antigens, CD34/immunology
MH  - Cell Differentiation/immunology
MH  - Cells, Cultured
MH  - Dendritic Cells/*cytology/immunology
MH  - Fetal Blood/*cytology
MH  - Hematopoietic Stem Cells/*cytology/immunology
MH  - Humans
MH  - Lymphocyte Activation
OTO - NOTNLM
OT  - CD34
OT  - dendritic cells
OT  - differentiation
OT  - hematopoietic stem cells
OT  - human umbilical cord blood
EDAT- 2015/05/16 06:00
MHDA- 2016/05/26 06:00
CRDT- 2015/05/16 06:00
PHST- 2015/02/21 00:00 [received]
PHST- 2015/05/05 00:00 [accepted]
PHST- 2015/05/16 06:00 [entrez]
PHST- 2015/05/16 06:00 [pubmed]
PHST- 2016/05/26 06:00 [medline]
AID - 10.1002/cbin.10490 [doi]
PST - ppublish
SO  - Cell Biol Int. 2015 Sep;39(9):1080-6. doi: 10.1002/cbin.10490. Epub 2015 Jul 24.