PMID- 25977235
OWN - NLM
STAT- MEDLINE
DCOM- 20160524
LR  - 20181113
IS  - 1460-2431 (Electronic)
IS  - 0022-0957 (Print)
IS  - 0022-0957 (Linking)
VI  - 66
IP  - 15
DP  - 2015 Aug
TI  - OsHrd3 is necessary for maintaining the quality of endoplasmic reticulum-derived 
      protein bodies in rice endosperm.
PG  - 4585-93
LID - 10.1093/jxb/erv229 [doi]
AB  - Large amounts of seed storage proteins (SSPs) are produced in the maturing 
      endosperm of rice seeds. Rice SSPs are synthesized as secretory proteins on the 
      rough endoplasmic reticulum (ER), and are transported and deposited into protein 
      complexes called protein bodies (PB-I and PB-II). Due to the high production of 
      SSPs, unfolded SSPs may be generated during this process. However, it was 
      previously unclear how such unfolded proteins are selected among synthesized 
      products and removed from the ER to maintain protein quality in the endosperm. 
      Since Hrd3/SEL1L recognizes unfolded proteins in yeast and mammalian protein 
      quality control systems, the role of OsHrd3 in protein quality control in rice 
      endosperm was investigated. Co-immunoprecipitation experiments demonstrated that 
      OsHrd3 interacts with components of the Hrd1 ubiquitin ligase complex such as 
      OsOS-9 and OsHrd1 in rice protoplasts. Endosperm-specific suppression of OsHrd3 
      in transgenic rice reduced the levels of polyubiquitinated proteins and resulted 
      in unfolded protein responses (UPRs) in the endosperm, suggesting that 
      OsHrd3-mediated polyubiquitination plays an important role in ER quality control. 
      It was found that a cysteine-rich 13kDa prolamin, RM1, was polyubiquitinated in 
      wild-type (WT) seeds but not in OsHrd3 knockdown (KD) seeds. RM1 formed aberrant 
      aggregates that were deposited abnormally in OsHrd3 KD seeds, resulting in 
      deformed PB-I. Therefore, the quality of protein bodies is maintained by 
      polyubiquitination of unfolded SSPs through the Hrd1 ubiquitin ligase system in 
      rice endosperm.
CI  - (c) The Author 2015. Published by Oxford University Press on behalf of the Society 
      for Experimental Biology.
FAU - Ohta, Masaru
AU  - Ohta M
AD  - Functional Transgenic Crops Research Unit, Genetically Modified Organism Research 
      Center, National Institute of Agrobiological Sciences, Kannondai 2-1-2, Tsukuba, 
      Ibaraki 305-8602, Japan.
FAU - Takaiwa, Fumio
AU  - Takaiwa F
AD  - Functional Transgenic Crops Research Unit, Genetically Modified Organism Research 
      Center, National Institute of Agrobiological Sciences, Kannondai 2-1-2, Tsukuba, 
      Ibaraki 305-8602, Japan takaiwa@affrc.go.jp.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150514
PL  - England
TA  - J Exp Bot
JT  - Journal of experimental botany
JID - 9882906
RN  - 0 (Plant Proteins)
RN  - 0 (Seed Storage Proteins)
SB  - IM
MH  - Endoplasmic Reticulum, Rough/*metabolism
MH  - Endosperm/metabolism
MH  - Oryza/*genetics/metabolism
MH  - Plant Proteins/*genetics/metabolism
MH  - Plants, Genetically Modified/genetics/metabolism
MH  - Promoter Regions, Genetic
MH  - Seed Storage Proteins/genetics/metabolism
PMC - PMC4507767
OTO - NOTNLM
OT  - Prolamins
OT  - protein body
OT  - protein quality control
OT  - seed storage protein
OT  - unfolded protein response.
EDAT- 2015/05/16 06:00
MHDA- 2016/05/25 06:00
PMCR- 2015/05/14
CRDT- 2015/05/16 06:00
PHST- 2015/05/16 06:00 [entrez]
PHST- 2015/05/16 06:00 [pubmed]
PHST- 2016/05/25 06:00 [medline]
PHST- 2015/05/14 00:00 [pmc-release]
AID - erv229 [pii]
AID - 10.1093/jxb/erv229 [doi]
PST - ppublish
SO  - J Exp Bot. 2015 Aug;66(15):4585-93. doi: 10.1093/jxb/erv229. Epub 2015 May 14.