PMID- 25977915
OWN - NLM
STAT- MEDLINE
DCOM- 20160204
LR  - 20181113
IS  - 2314-6141 (Electronic)
IS  - 2314-6133 (Print)
VI  - 2015
DP  - 2015
TI  - Binding of citreoviridin to human serum albumin: multispectroscopic and molecular 
      docking.
PG  - 162391
LID - 10.1155/2015/162391 [doi]
LID - 162391
AB  - Citreoviridin (CIT), a mycotoxin produced by Penicillium citreonigrum, is a 
      common contaminant of wide range of agriproducts and detrimental to human and 
      animal health. In this study, the interaction of CIT with human serum albumin 
      (HSA) is researched by steady-state fluorescence, ultraviolet-visible (UV-Vis) 
      absorption, circular dichroism (CD) methods, and molecular modeling. The 
      association constants, binding site numbers, and corresponding thermodynamic 
      parameters are used to investigate the quenching mechanism. The alternations of 
      HSA secondary structure in the presence of CIT are demonstrated with UV-Vis, 
      synchronous fluorescence, and CD spectra. The molecular modeling results reveal 
      that CIT can bind with hydrophobic pocket of HSA with hydrophobic and hydrogen 
      bond force. Moreover, an apparent distance of 3.25 nm between Trp214 and CIT is 
      obtained via fluorescence resonance energy transfer method.
FAU - Hou, Haifeng
AU  - Hou H
AD  - School of Public Health, Shandong University, Jinan 250012, China ; School of 
      Public Health, Taishan Medical University, Taian 271016, China.
FAU - Qu, Xiaolan
AU  - Qu X
AD  - School of Pharmacy, Taishan Medical University, Taian 271016, China.
FAU - Li, Yuqin
AU  - Li Y
AD  - School of Pharmacy, Taishan Medical University, Taian 271016, China.
FAU - Kong, Yueyue
AU  - Kong Y
AD  - School of Pharmacy, Taishan Medical University, Taian 271016, China.
FAU - Jia, Baoxiu
AU  - Jia B
AD  - School of Pharmacy, Taishan Medical University, Taian 271016, China.
FAU - Yao, Xiaojun
AU  - Yao X
AD  - School of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou 271000, 
      China.
FAU - Jiang, Baofa
AU  - Jiang B
AD  - School of Public Health, Shandong University, Jinan 250012, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150421
PL  - United States
TA  - Biomed Res Int
JT  - BioMed research international
JID - 101600173
RN  - 0 (Aurovertins)
RN  - 0 (Serum Albumin)
RN  - OWX7Q6CF4F (citreoviridin)
SB  - IM
MH  - Aurovertins/*chemistry/metabolism
MH  - Binding Sites
MH  - Circular Dichroism
MH  - Humans
MH  - Hydrogen Bonding
MH  - Hydrophobic and Hydrophilic Interactions
MH  - *Models, Molecular
MH  - Molecular Docking Simulation
MH  - Penicillium/chemistry/pathogenicity
MH  - Protein Binding
MH  - Serum Albumin/*chemistry/metabolism
MH  - Spectrophotometry, Ultraviolet
MH  - Thermodynamics
PMC - PMC4419221
EDAT- 2015/05/16 06:00
MHDA- 2016/02/05 06:00
PMCR- 2015/04/21
CRDT- 2015/05/16 06:00
PHST- 2015/02/17 00:00 [received]
PHST- 2015/03/25 00:00 [revised]
PHST- 2015/03/27 00:00 [accepted]
PHST- 2015/05/16 06:00 [entrez]
PHST- 2015/05/16 06:00 [pubmed]
PHST- 2016/02/05 06:00 [medline]
PHST- 2015/04/21 00:00 [pmc-release]
AID - 10.1155/2015/162391 [doi]
PST - ppublish
SO  - Biomed Res Int. 2015;2015:162391. doi: 10.1155/2015/162391. Epub 2015 Apr 21.