PMID- 25980610
OWN - NLM
STAT- MEDLINE
DCOM- 20150930
LR  - 20151119
IS  - 1873-3468 (Electronic)
IS  - 0014-5793 (Linking)
VI  - 589
IP  - 15
DP  - 2015 Jul 8
TI  - Lymphotoxin alpha, a novel target of posttranscriptional gene regulation by HuR in 
      HepG2 cells.
PG  - 1943-50
LID - S0014-5793(15)00391-9 [pii]
LID - 10.1016/j.febslet.2015.05.015 [doi]
AB  - The role of the RNA-binding protein human antigen R (HuR) in hepatocarcinogenesis 
      is still elusive. By employing short hairpin (sh)RNA-dependent knockdown 
      approach, we demonstrate that lymphotoxin alpha (LTalpha) is a target of 
      posttranscriptional gene regulation by HuR in hepatocellular carcinoma (HepG2) 
      cells. Consequently, the increased mRNA decay upon HuR depletion significantly 
      affects lymphotoxin expression at both, the mRNA and protein level. 
      Biotin-pulldown assay showed that HuR specifically interacts with the 
      3'-untranslated region (3'-UTR) of the LTalpha mRNA. Furthermore, electrophoretic 
      mobility shift assay (EMSA) implicates that the RNA-binding critically depends on 
      the RNA recognition motif 2 (RRM2) and the hinge region of HuR.
CI  - Copyright (c) 2015 Federation of European Biochemical Societies. Published by 
      Elsevier B.V. All rights reserved.
FAU - Steinmeyer, Nico
AU  - Steinmeyer N
AD  - pharmazentrum frankfurt/ZAFES, Klinikum der Goethe-Universitat Frankfurt, 
      Frankfurt am Main, Germany.
FAU - Doller, Anke
AU  - Doller A
AD  - Industrielle Biotechnologie, Fakultat Ingenieurwissenschaften, Hochschule 
      Ansbach, Germany.
FAU - Biyanee, Abhiruchi
AU  - Biyanee A
AD  - pharmazentrum frankfurt/ZAFES, Klinikum der Goethe-Universitat Frankfurt, 
      Frankfurt am Main, Germany.
FAU - Brauss, Thilo
AU  - Brauss T
AD  - Institut fur Biochemie I (Pathobiochemie), Klinikum der Goethe-Universitat 
      Frankfurt, Frankfurt am Main, Germany.
FAU - Schmid, Tobias
AU  - Schmid T
AD  - Institut fur Biochemie I (Pathobiochemie), Klinikum der Goethe-Universitat 
      Frankfurt, Frankfurt am Main, Germany.
FAU - Pfeilschifter, Josef
AU  - Pfeilschifter J
AD  - pharmazentrum frankfurt/ZAFES, Klinikum der Goethe-Universitat Frankfurt, 
      Frankfurt am Main, Germany.
FAU - Eberhardt, Wolfgang
AU  - Eberhardt W
AD  - pharmazentrum frankfurt/ZAFES, Klinikum der Goethe-Universitat Frankfurt, 
      Frankfurt am Main, Germany. Electronic address: w.eberhardt@em.uni-frankfurt.de.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150514
PL  - England
TA  - FEBS Lett
JT  - FEBS letters
JID - 0155157
RN  - 0 (3' Untranslated Regions)
RN  - 0 (ELAV Proteins)
RN  - 0 (Lymphotoxin-alpha)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - 3' Untranslated Regions
MH  - ELAV Proteins/genetics/*physiology
MH  - Electrophoretic Mobility Shift Assay
MH  - *Gene Expression Regulation
MH  - Gene Knockdown Techniques
MH  - Hep G2 Cells
MH  - Humans
MH  - Lymphotoxin-alpha/*genetics/metabolism
MH  - *RNA Processing, Post-Transcriptional
MH  - RNA, Messenger/genetics
MH  - Real-Time Polymerase Chain Reaction
OTO - NOTNLM
OT  - AU-rich element
OT  - Hepatocellular carcinoma
OT  - Lymphotoxin alpha
OT  - mRNA-stability
EDAT- 2015/05/20 06:00
MHDA- 2015/10/01 06:00
CRDT- 2015/05/19 06:00
PHST- 2015/03/03 00:00 [received]
PHST- 2015/04/24 00:00 [revised]
PHST- 2015/05/06 00:00 [accepted]
PHST- 2015/05/19 06:00 [entrez]
PHST- 2015/05/20 06:00 [pubmed]
PHST- 2015/10/01 06:00 [medline]
AID - S0014-5793(15)00391-9 [pii]
AID - 10.1016/j.febslet.2015.05.015 [doi]
PST - ppublish
SO  - FEBS Lett. 2015 Jul 8;589(15):1943-50. doi: 10.1016/j.febslet.2015.05.015. Epub 
      2015 May 14.