PMID- 25981216
OWN - NLM
STAT- MEDLINE
DCOM- 20160425
LR  - 20220410
IS  - 1432-1084 (Electronic)
IS  - 0938-7994 (Linking)
VI  - 25
IP  - 10
DP  - 2015 Oct
TI  - In-vivo monitoring of acute DSS-Colitis using Colonoscopy, high resolution 
      Ultrasound and bench-top Magnetic Resonance Imaging in Mice.
PG  - 2984-91
LID - 10.1007/s00330-015-3714-3 [doi]
AB  - OBJECTIVE: The aim of this study was to establish and evaluate (colour Doppler-) 
      high-resolution-ultrasound (hrUS) and bench-top magnetic resonance imaging 
      (btMRI) as new methods to monitor experimental colitis. MATERIALS AND METHODS: 
      hrUS, btMRI and endoscopy were performed in mice without colitis (n = 15), in 
      mice with acute colitis (n = 14) and in mice with acute colitis and simultaneous 
      treatment with infliximab (n = 19). RESULTS: Determination of colon wall 
      thickness using hrUS (32 MHz) and measurement of the cross-sectional colonic 
      areas by btMRI allowed discrimination between the treatment groups (mean a vs. b 
      vs. c - btMRI: 922 vs. 2051 vs. 1472 pixel, hrUS: 0.26 vs. 0.45 vs. 0.31 mm). 
      btMRI, endoscopy, hrUS and colour Doppler-hrUS correlated to histological scoring 
      (p < 0.05), while endoscopy and btMRI correlated to post-mortem colon length 
      (p < 0.05). CONCLUSIONS: The innovative in vivo techniques btMRI and hrUS are 
      safe and technically feasible. They differentiate between distinct grades of 
      colitis in an experimental setting, and correlate with established post-mortem 
      parameters. In addition to endoscopic procedures, these techniques provide 
      information regarding colon wall thickness and perfusion. Depending on the 
      availability of these techniques, their application increases the value of in 
      vivo monitoring in experimental acute colitis in small rodents. KEY POINTS: * 
      Improved in vivo monitoring might balance interindividual differences in murine 
      colitis. * In monitoring murine colitis, btMRI and hrUS are safe and technically 
      feasible. * Very short examination times underline the usefulness especially of 
      hrUS. * Results of btMRI and hrUS correlate with endoscopic and post-mortem 
      findings.
FAU - Walldorf, J
AU  - Walldorf J
AD  - Department of Internal Medicine I, Martin Luther University Halle-Wittenberg, 
      Ernst-Grube-Strasse 40, 06120, Halle, Germany, 
      jens.walldorf@medizin.uni-halle.de.
FAU - Hermann, M
AU  - Hermann M
FAU - Porzner, M
AU  - Porzner M
FAU - Pohl, S
AU  - Pohl S
FAU - Metz, H
AU  - Metz H
FAU - Mader, K
AU  - Mader K
FAU - Zipprich, A
AU  - Zipprich A
FAU - Christ, B
AU  - Christ B
FAU - Seufferlein, T
AU  - Seufferlein T
LA  - eng
PT  - Journal Article
DEP - 20150517
PL  - Germany
TA  - Eur Radiol
JT  - European radiology
JID - 9114774
RN  - 0 (Gastrointestinal Agents)
RN  - 0 (Irritants)
RN  - 9042-14-2 (Dextran Sulfate)
RN  - B72HH48FLU (Infliximab)
SB  - IM
MH  - Acute Disease
MH  - Animals
MH  - Colitis/chemically induced/*diagnosis
MH  - Colon/blood supply
MH  - Colonoscopy/methods
MH  - Cross-Sectional Studies
MH  - Dextran Sulfate/toxicity
MH  - Diagnosis, Differential
MH  - Feasibility Studies
MH  - Gastrointestinal Agents/pharmacology
MH  - Infliximab/pharmacology
MH  - Irritants/toxicity
MH  - Magnetic Resonance Imaging/methods
MH  - Male
MH  - Mice, Inbred BALB C
MH  - Multimodal Imaging/methods
MH  - Ultrasonography, Doppler, Color
EDAT- 2015/05/20 06:00
MHDA- 2016/04/26 06:00
CRDT- 2015/05/19 06:00
PHST- 2014/08/17 00:00 [received]
PHST- 2015/03/11 00:00 [accepted]
PHST- 2015/01/07 00:00 [revised]
PHST- 2015/05/19 06:00 [entrez]
PHST- 2015/05/20 06:00 [pubmed]
PHST- 2016/04/26 06:00 [medline]
AID - 10.1007/s00330-015-3714-3 [doi]
PST - ppublish
SO  - Eur Radiol. 2015 Oct;25(10):2984-91. doi: 10.1007/s00330-015-3714-3. Epub 2015 
      May 17.