PMID- 25981750
OWN - NLM
STAT- MEDLINE
DCOM- 20161018
LR  - 20221207
IS  - 1464-5491 (Electronic)
IS  - 0742-3071 (Linking)
VI  - 33
IP  - 1
DP  - 2016 Jan
TI  - Elevated serum monocyte chemoattractant protein-1 levels and its genetic 
      polymorphism is associated with diabetic retinopathy in Chinese patients with 
      Type 2 diabetes.
PG  - 84-90
LID - 10.1111/dme.12804 [doi]
AB  - AIM: Previous studies have reported that monocyte chemoattractant protein-1 
      (MCP-1) is involved in inflammatory and metabolic diseases. The purpose of this 
      study is to investigate the role of MCP-1 in the pathogenesis of diabetic 
      retinopathy (DR) in Han Chinese patients with Type 2 diabetes. METHODS: Serum 
      levels of MCP-1 protein in patients classified as diabetic without retinopathy 
      (DWR) and DR, including NPDR and PDR, were assayed by enzyme-linked immunosorbent 
      assay. Genomic DNA from 198 DWR patients, 176 NPDR patients and 143 PDR patients 
      were genotyped by using a PCR restriction fragment length polymorphism (PCR-RFLP) 
      assay. RESULTS: MCP-1 serum levels were significantly higher in NPDR and PDR 
      patients than in the DWR patients. The frequencies of the GG genotype and G 
      allele of the single nucleotide polymorphism (SNP) were significantly increased 
      in DR patients compared with DWR patients. Further subgroup analysis was 
      performed to test whether there was an association between the PDR or NPDR and 
      DWR groups. Significantly higher frequencies of the GG genotype and G allele were 
      observed in PDR and NPDR patients compared with DWR patients. Furthermore, the 25 
      patients with PDR were divided into three groups according to the genotype of the 
      tested SNP. The expression of the MCP-1 gene was higher in the GG genotype group 
      compared with the other two groups. CONCLUSIONS: The results suggested that the 
      -2518 GG genotype and G allele of MCP-1 are associated with an increased risk of 
      PDR in the Chinese Han population. This polymorphism may influence the expression 
      of the MCP-1 gene, which may play an important role in the pathogenesis of DR.
CI  - (c) 2015 The Authors. Diabetic Medicine (c) 2015 Diabetes UK.
FAU - Jiang, Z
AU  - Jiang Z
AD  - Department of Ophthalmology, Second Affiliated Hospital of Anhui Medical 
      University, Hefei, Anhui, China.
FAU - Hennein, L
AU  - Hennein L
AD  - Department of Ophthalmology, University of California San Francisco, San 
      Francisco, USA.
AD  - University of California San Francisco, School of Medicine, San Francisco, USA.
FAU - Xu, Y
AU  - Xu Y
AD  - Department of Ophthalmology, Second Affiliated Hospital of Anhui Medical 
      University, Hefei, Anhui, China.
FAU - Bao, N
AU  - Bao N
AD  - Department of Ophthalmology, Second Affiliated Hospital of Anhui Medical 
      University, Hefei, Anhui, China.
FAU - Coh, P
AU  - Coh P
AD  - Department of Ophthalmology, University of California San Francisco, San 
      Francisco, USA.
FAU - Tao, L
AU  - Tao L
AD  - Department of Ophthalmology, Second Affiliated Hospital of Anhui Medical 
      University, Hefei, Anhui, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150613
PL  - England
TA  - Diabet Med
JT  - Diabetic medicine : a journal of the British Diabetic Association
JID - 8500858
RN  - 0 (5' Untranslated Regions)
RN  - 0 (CCL2 protein, human)
RN  - 0 (Chemokine CCL2)
SB  - IM
MH  - *5' Untranslated Regions
MH  - Adult
MH  - Alleles
MH  - Asian People
MH  - Case-Control Studies
MH  - Chemokine CCL2/blood/*genetics/metabolism
MH  - China
MH  - Diabetes Mellitus, Type 2/blood/*complications/immunology/metabolism
MH  - Diabetic Retinopathy/blood/*genetics/immunology/metabolism
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Female
MH  - Gene Expression Regulation
MH  - Genetic Association Studies
MH  - *Genetic Predisposition to Disease
MH  - Hospitals, University
MH  - Humans
MH  - Male
MH  - Middle Aged
MH  - Polymorphism, Restriction Fragment Length
MH  - *Polymorphism, Single Nucleotide
MH  - *Up-Regulation
EDAT- 2015/05/20 06:00
MHDA- 2016/10/19 06:00
CRDT- 2015/05/19 06:00
PHST- 2015/05/13 00:00 [accepted]
PHST- 2015/05/19 06:00 [entrez]
PHST- 2015/05/20 06:00 [pubmed]
PHST- 2016/10/19 06:00 [medline]
AID - 10.1111/dme.12804 [doi]
PST - ppublish
SO  - Diabet Med. 2016 Jan;33(1):84-90. doi: 10.1111/dme.12804. Epub 2015 Jun 13.