PMID- 25981900 OWN - NLM STAT- MEDLINE DCOM- 20160224 LR - 20150603 IS - 1573-7217 (Electronic) IS - 0167-6806 (Linking) VI - 151 IP - 3 DP - 2015 Jun TI - Status of estrogen receptor 1 (ESR1) gene in mastopathy predicts subsequent development of breast cancer. PG - 709-15 LID - 10.1007/s10549-015-3427-y [doi] AB - Mastopathy is a common disease of the breast likely associated with elevated estrogen levels and a putative risk factor for breast cancer. The role of estrogen receptor alpha (ESR1) in mastopathy has not been investigated previously. Here, we investigated the prevalence of ESR1 gene amplification in mastopathy and its prediction for breast cancer. Paraffin-embedded tissues from 58 women with invasive breast cancer were analyzed. For all women, tissues with mastopathy taken at least 1.5 years before first diagnosis of breast cancer were available. Tissue from 46 women with mastopathy without a diagnosis of breast carcinoma in the observed time frame (12-18 years) was used as control. Fluorescence in situ hybridization analysis revealed that ESR1 was amplified in nine of 58 (15.5 %) breast cancers. All ESR1-amplified breast cancers were strongly positive for estrogen receptor with ER immunohistochemistry. Interestingly, in women with ESR1 amplification in breast cancer, the amplification was detectable in mastopathic tissues prior to the first diagnosis of breast cancer but was absent in tissues from women with mastopathy who did not develop breast cancer. Our study suggests that ESR1 gene amplification is an early event in breast pathology and might be a helpful predictive marker to identify patients at high risk of developing breast cancer. FAU - Soysal, Savas D AU - Soysal SD AD - Department of Surgery, University Hospital Basel, Spitalstrasse 21, 4031, Basel, Switzerland, S.SOYSAL@gmx.de. FAU - Kilic, Incken B AU - Kilic IB FAU - Regenbrecht, Christian R A AU - Regenbrecht CR FAU - Schneider, Sandra AU - Schneider S FAU - Muenst, Simone AU - Muenst S FAU - Kilic, Nerbil AU - Kilic N FAU - Guth, Uwe AU - Guth U FAU - Dietel, Manfred AU - Dietel M FAU - Terracciano, Luigi M AU - Terracciano LM FAU - Kilic, Ergin AU - Kilic E LA - eng PT - Journal Article DEP - 20150516 PL - Netherlands TA - Breast Cancer Res Treat JT - Breast cancer research and treatment JID - 8111104 RN - 0 (Biomarkers, Tumor) RN - 0 (Estrogen Receptor alpha) SB - IM MH - Aged MH - Aged, 80 and over MH - Biomarkers, Tumor MH - Breast Diseases/*complications/*genetics/pathology/surgery MH - Breast Neoplasms/diagnosis/*epidemiology/*etiology MH - Cohort Studies MH - Estrogen Receptor alpha/*genetics/metabolism MH - Female MH - Follow-Up Studies MH - Gene Amplification MH - *Gene Expression MH - *Genetic Predisposition to Disease MH - Humans MH - Immunohistochemistry MH - In Situ Hybridization, Fluorescence MH - Middle Aged MH - ROC Curve EDAT- 2015/05/20 06:00 MHDA- 2016/02/26 06:00 CRDT- 2015/05/19 06:00 PHST- 2015/01/06 00:00 [received] PHST- 2015/05/11 00:00 [accepted] PHST- 2015/05/19 06:00 [entrez] PHST- 2015/05/20 06:00 [pubmed] PHST- 2016/02/26 06:00 [medline] AID - 10.1007/s10549-015-3427-y [doi] PST - ppublish SO - Breast Cancer Res Treat. 2015 Jun;151(3):709-15. doi: 10.1007/s10549-015-3427-y. Epub 2015 May 16.