PMID- 2598473
OWN - NLM
STAT- MEDLINE
DCOM- 19900131
LR  - 20190813
IS  - 0300-0664 (Print)
IS  - 0300-0664 (Linking)
VI  - 30
IP  - 4
DP  - 1989 Apr
TI  - Corticotrophin-releasing factor-like immunoreactivity in human 24 h urine.
PG  - 405-12
AB  - This report is the first demonstration of the presence of 
      corticotrophin-releasing factor-like immunoreactive material (CRF-LI) in human 
      urine. Our studies, using immunoaffinity chromatography for extraction, followed 
      by reverse phase high performance liquid chromatography for further purification, 
      and radioimmunoassay for final peptide measurement, suggest that CRF-LI from 
      human urine is similar, if not identical, to human CRF. Urinary CRF-LI excretion 
      in normal subjects was 1.48 +/- 0.69 ng/24 h (mean +/- SD; n = 20) with no 
      significant differences between the sexes. During pregnancy, urinary CRF-LI 
      excretion increased, and a significant correlation between urinary CRF-LI 
      excretion and the gestational age was seen (r = 0.62; P less than 0.0001). We 
      suggest that urinary 24-h CRF-LI excretion may reflect the secretion of CRF into 
      the circulation. Measurements of CRF-LI in the urine may have several advantages 
      over determinations of hormone levels in plasma.
FAU - Maser-Gluth, C
AU  - Maser-Gluth C
AD  - Department of Pharmacology, University of Heidelberg, FR Germany.
FAU - Vecsei, P
AU  - Vecsei P
LA  - eng
PT  - Journal Article
PL  - England
TA  - Clin Endocrinol (Oxf)
JT  - Clinical endocrinology
JID - 0346653
RN  - 9015-71-8 (Corticotropin-Releasing Hormone)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Chromatography, Affinity
MH  - Chromatography, High Pressure Liquid
MH  - Corticotropin-Releasing Hormone/*urine
MH  - Female
MH  - Humans
MH  - Pregnancy
MH  - Radioimmunoassay
EDAT- 1989/04/01 00:00
MHDA- 1989/04/01 00:01
CRDT- 1989/04/01 00:00
PHST- 1989/04/01 00:00 [pubmed]
PHST- 1989/04/01 00:01 [medline]
PHST- 1989/04/01 00:00 [entrez]
AID - 10.1111/j.1365-2265.1989.tb00439.x [doi]
PST - ppublish
SO  - Clin Endocrinol (Oxf). 1989 Apr;30(4):405-12. doi: 
      10.1111/j.1365-2265.1989.tb00439.x.