PMID- 25985917
OWN - NLM
STAT- MEDLINE
DCOM- 20160406
LR  - 20150717
IS  - 1523-6536 (Electronic)
IS  - 1083-8791 (Linking)
VI  - 21
IP  - 8
DP  - 2015 Aug
TI  - Granulocyte Colony-Stimulating Factor Induces Osteoblast Inhibition by B 
      Lymphocytes and Osteoclast Activation by T Lymphocytes during Hematopoietic 
      Stem/Progenitor Cell Mobilization.
PG  - 1384-91
LID - S1083-8791(15)00328-6 [pii]
LID - 10.1016/j.bbmt.2015.05.005 [doi]
AB  - In the bone marrow (BM), hematopoietic stem and progenitor cells (HSPCs) reside 
      in specialized niches near osteoblast cells at the endosteum. HSPCs that egress 
      to peripheral blood are widely used for transplant, and mobilization is most 
      commonly performed with recombinant human granulocyte colony-stimulating factor 
      (G-CSF). However, the cellular targets of G-CSF that initiate the mobilization 
      cascade and bone remodeling are not completely understood. Here, we examined 
      whether T and B lymphocytes modulate the bone niche and influence HSPC 
      mobilization. We used T and B defective mice to show that G-CSF-induced 
      mobilization of HSPCs correlated with B lymphocytes but poorly with T 
      lymphocytes. In addition, we found that defective B lymphocytes prevent 
      G-CSF-mediated osteoblast disruption, and further study showed BM osteoblasts 
      were reduced coincident with mobilization, induced by elevated expression of 
      dickkopf1 of BM B lymphocytes. BM T cells were also involved in G-CSF-induced 
      osteoclast activation by regulating the Receptor Activator of Nuclear Factor-kappa B 
      Ligand/Osteoprotegerin (RANKL/OPG) axis. These data provide evidence that BM B 
      and T lymphocytes play a role in G-CSF-induced HSPC mobilization by regulating 
      bone remodeling.
CI  - Copyright (c) 2015 American Society for Blood and Marrow Transplantation. Published 
      by Elsevier Inc. All rights reserved.
FAU - Li, Sidan
AU  - Li S
AD  - Beijing Key Laboratory of Pediatric Hematology Oncology; National Key Discipline 
      of Pediatrics, Ministry of Education; Key Laboratory of Major Diseases in 
      Children, Ministry of Education; Hematology Oncology Center, Beijing Children's 
      Hospital, Capital Medical University, Beijing, China; State Key Laboratory of 
      Experimental Hematology, Institute of Hematology and Hospital of Blood Diseases, 
      Chinese Academy of Medical Sciences and Peking Union of Medical College, Tianjin, 
      China.
FAU - Li, Tianshou
AU  - Li T
AD  - Department of Hematology, The First Affiliated Hospital of Guangxi Medical 
      University, Guangxi, China.
FAU - Chen, Yongbing
AU  - Chen Y
AD  - Department of Hepatobiliary Surgery, General Hospital of Beijing Military Area 
      Command, Beijing, China.
FAU - Nie, Yinchao
AU  - Nie Y
AD  - Department of Hematology, The First Affiliated Hospital of Guangxi Medical 
      University, Guangxi, China.
FAU - Li, Changhong
AU  - Li C
AD  - State Key Laboratory of Experimental Hematology, Institute of Hematology and 
      Hospital of Blood Diseases, Chinese Academy of Medical Sciences and Peking Union 
      of Medical College, Tianjin, China.
FAU - Liu, Lanting
AU  - Liu L
AD  - State Key Laboratory of Experimental Hematology, Institute of Hematology and 
      Hospital of Blood Diseases, Chinese Academy of Medical Sciences and Peking Union 
      of Medical College, Tianjin, China.
FAU - Li, Qiaochuan
AU  - Li Q
AD  - Department of Hematology, The First Affiliated Hospital of Guangxi Medical 
      University, Guangxi, China. Electronic address: liqiaochuan@sohu.com.
FAU - Qiu, Lugui
AU  - Qiu L
AD  - State Key Laboratory of Experimental Hematology, Institute of Hematology and 
      Hospital of Blood Diseases, Chinese Academy of Medical Sciences and Peking Union 
      of Medical College, Tianjin, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150516
PL  - United States
TA  - Biol Blood Marrow Transplant
JT  - Biology of blood and marrow transplantation : journal of the American Society for 
      Blood and Marrow Transplantation
JID - 9600628
RN  - 143011-72-7 (Granulocyte Colony-Stimulating Factor)
SB  - IM
MH  - Animals
MH  - B-Lymphocytes/*metabolism
MH  - Granulocyte Colony-Stimulating Factor/*metabolism/pharmacology
MH  - Hematopoietic Stem Cell Mobilization/*methods
MH  - Humans
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Osteoblasts/*metabolism
MH  - Osteoclasts/*metabolism
MH  - T-Lymphocytes/*metabolism
OTO - NOTNLM
OT  - Hematopoietic stem/progenitor cells
OT  - Lymphocyte
OT  - Stem cell niche
EDAT- 2015/05/20 06:00
MHDA- 2016/04/07 06:00
CRDT- 2015/05/20 06:00
PHST- 2015/02/21 00:00 [received]
PHST- 2015/05/04 00:00 [accepted]
PHST- 2015/05/20 06:00 [entrez]
PHST- 2015/05/20 06:00 [pubmed]
PHST- 2016/04/07 06:00 [medline]
AID - S1083-8791(15)00328-6 [pii]
AID - 10.1016/j.bbmt.2015.05.005 [doi]
PST - ppublish
SO  - Biol Blood Marrow Transplant. 2015 Aug;21(8):1384-91. doi: 
      10.1016/j.bbmt.2015.05.005. Epub 2015 May 16.