PMID- 25986633
OWN - NLM
STAT- MEDLINE
DCOM- 20151215
LR  - 20200227
IS  - 1465-2099 (Electronic)
IS  - 0022-1317 (Linking)
VI  - 96
IP  - 9
DP  - 2015 Sep
TI  - Mutation of UL24 impedes the dissemination of acute herpes simplex virus 1 
      infection from the cornea to neurons of trigeminal ganglia.
PG  - 2794-2805
LID - 10.1099/vir.0.000189 [doi]
AB  - Herpes simplex virus 1 (human herpesvirus 1) initially infects epithelial cells 
      of the mucosa and then goes on to infect sensory neurons leading ultimately to a 
      latent infection in trigeminal ganglia (TG). UL24 is a core herpesvirus gene that 
      has been identified as a determinant of pathogenesis in several 
      Alphaherpesvirinae, although the underlying mechanisms are unknown. In a mouse 
      model of ocular infection, a UL24-deficient virus exhibited a reduction in viral 
      titres in tear films of 1 log10, whilst titres in TG are often below the level of 
      detection. Moreover, the efficiency of reactivation from latency was also 
      severely reduced. Herein, we investigated how UL24 contributed to acute infection 
      of TG. Our results comparing the impact of UL24 on viral titres in eye tissue 
      versus in tear films did not reveal a general defect in virus release from the 
      cornea. We also found that the impairment of replication seen in mouse primary 
      embryonic neurons with a UL24-deficient virus was not more severe than that 
      observed in an epithelial cell line. Rather, in situ histological analyses 
      revealed that infection with a UL24-deficient virus led to a significant 
      reduction in the number of acutely infected neurons at 3 days post-infection 
      (p.i.). Moreover, there was a significant reduction in the number of neurons 
      positive for viral DNA at 2 days p.i. for the UL24-deficient virus as compared 
      with that observed for WT or a rescue virus. Our results supported a model 
      whereby UL24 functions in the dissemination of acute infection from the cornea to 
      neurons in TG.
FAU - Rochette, Pierre-Alexandre
AU  - Rochette PA
AD  - Universite INRS, INRS-Institut Armand-Frappier, 531 boulevard des Prairies, 
      Laval, Quebec H7V 1B7, Canada.
FAU - Bourget, Amelie
AU  - Bourget A
AD  - Universite INRS, INRS-Institut Armand-Frappier, 531 boulevard des Prairies, 
      Laval, Quebec H7V 1B7, Canada.
FAU - Sanabria-Solano, Carolina
AU  - Sanabria-Solano C
AD  - Universite INRS, INRS-Institut Armand-Frappier, 531 boulevard des Prairies, 
      Laval, Quebec H7V 1B7, Canada.
FAU - Lahmidi, Soumia
AU  - Lahmidi S
AD  - Universite INRS, INRS-Institut Armand-Frappier, 531 boulevard des Prairies, 
      Laval, Quebec H7V 1B7, Canada.
FAU - Lavallee, Gabriel Ouellet
AU  - Lavallee GO
AD  - Universite INRS, INRS-Institut Armand-Frappier, 531 boulevard des Prairies, 
      Laval, Quebec H7V 1B7, Canada.
FAU - Pearson, Angela
AU  - Pearson A
AD  - Universite INRS, INRS-Institut Armand-Frappier, 531 boulevard des Prairies, 
      Laval, Quebec H7V 1B7, Canada.
LA  - eng
GR  - MOP 82924/Canadian Institutes of Health Research/Canada
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150518
PL  - England
TA  - J Gen Virol
JT  - The Journal of general virology
JID - 0077340
RN  - 0 (UL24 protein, Human herpesvirus 1)
RN  - 0 (Viral Proteins)
SB  - IM
MH  - Animals
MH  - Cornea/*virology
MH  - Disease Models, Animal
MH  - Herpes Simplex/*virology
MH  - Herpesvirus 1, Human/genetics/*physiology
MH  - Humans
MH  - Mice
MH  - Mutation
MH  - Neurons/*virology
MH  - Trigeminal Ganglion/cytology/*virology
MH  - Viral Proteins/*genetics/metabolism
MH  - *Virus Replication
EDAT- 2015/05/20 06:00
MHDA- 2015/12/17 06:00
CRDT- 2015/05/20 06:00
PHST- 2015/05/20 06:00 [entrez]
PHST- 2015/05/20 06:00 [pubmed]
PHST- 2015/12/17 06:00 [medline]
AID - 10.1099/vir.0.000189 [doi]
PST - ppublish
SO  - J Gen Virol. 2015 Sep;96(9):2794-2805. doi: 10.1099/vir.0.000189. Epub 2015 May 
      18.