PMID- 25987069
OWN - NLM
STAT- MEDLINE
DCOM- 20160216
LR  - 20190606
IS  - 2476-762X (Electronic)
IS  - 1513-7368 (Linking)
VI  - 16
IP  - 9
DP  - 2015
TI  - Activities of E6 Protein of Human Papillomavirus 16 Asian Variant on miR-21 
      Up-regulation and Expression of Human Immune Response Genes.
PG  - 3961-8
AB  - BACKGROUND: Variants of human papillomavirus (HPV) show more oncogenicity than do 
      prototypes. The HPV16 Asian variant (HPV16As) plays a major role in cervical 
      cancer of Asian populations. Some amino acid changes in the E6 protein of HPV16 
      variants affect E6 functions such as p53 interaction and host immune 
      surveillance. This study aimed to investigate activities of HPV16As E6 protein on 
      modulation of expression of miRNA-21 as well as interferon regulatory factors 
      (IRFs) 1, 3, 7 and c-fos. MATERIALS AND METHODS: Vectors expressing E6 protein of 
      HPV16As (E6D25E) or HPV16 prototype (E6Pro) were constructed and transfected into 
      C33A cells. HCK1T cells expressing E6D25E or E6Pro were established by 
      transducing retrovirus-containing E6D25E or 16E6Pro. The E6AP-binding activity of 
      E6 and proliferation of the transfected C33A cells were determined. MiR-21 and 
      mRNA of interesting genes were detected in the transfected C33A cells and/or the 
      HCK1T cells, with or without treatment by culture medium from HeLa cells 
      (HeLa-CM). RESULTS: E6D25E showed binding activity with E6AP similar to that of 
      E6Pro. Interestingly, E6D25E showed a higher activity of miR-21 induction than 
      did E6Pro in C33A cells expressing E6 protein. This result was similar to the 
      HCK1T cells expressing E6 protein, with HeLa-CM treatment. The miR-21 
      up-regulation significantly corresponded to its target expression. Different 
      levels of expression of IRFs were also observed in the HCK1T cells expressing E6 
      protein. Interestingly, when treated with HeLa-CM, IRFs 1, 3 and 7 as well as 
      c-fos were significantly suppressed in the HCK1T cells expressing E6D25E, whereas 
      those in the HCK1T cells expressing E6Pro were induced. A similar situation was 
      seen for IFN-alpha and IFN-beta. CONCLUSIONS: E6D25E of the HPV16As variant differed 
      from the E6 prototype in its activities on epigenetic modulation and immune 
      surveillance and this might be a key factor for the important role of this 
      variant in cervical cancer progression.
FAU - Chopjitt, Peechanika
AU  - Chopjitt P
AD  - Department of Microbiology, Faculty of Medicine, 3HPV and EBV and Carcinogenesis 
      Research Group, Khon Kaen University, Khon Kaen, Thailand E-mail : 
      tipeka@kku.ac.th.
FAU - Pientong, Chamsai
AU  - Pientong C
FAU - Bumrungthai, Sureewan
AU  - Bumrungthai S
FAU - Kongyingyoes, Bunkerd
AU  - Kongyingyoes B
FAU - Ekalaksananan, Tipaya
AU  - Ekalaksananan T
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Thailand
TA  - Asian Pac J Cancer Prev
JT  - Asian Pacific journal of cancer prevention : APJCP
JID - 101130625
RN  - 0 (E6 protein, Human papillomavirus type 16)
RN  - 0 (IRF3 protein, human)
RN  - 0 (Interferon Regulatory Factor-1)
RN  - 0 (Interferon Regulatory Factor-3)
RN  - 0 (Interferon Regulatory Factor-7)
RN  - 0 (MIRN21 microRNA, human)
RN  - 0 (MicroRNAs)
RN  - 0 (Oncogene Proteins, Viral)
RN  - 0 (Proto-Oncogene Proteins c-fos)
RN  - 0 (RNA, Messenger)
RN  - 0 (Repressor Proteins)
SB  - IM
MH  - Blotting, Western
MH  - Cell Cycle
MH  - Cell Proliferation
MH  - Female
MH  - HeLa Cells
MH  - Humans
MH  - Interferon Regulatory Factor-1/genetics/*metabolism
MH  - Interferon Regulatory Factor-3/genetics/*metabolism
MH  - Interferon Regulatory Factor-7/genetics/*metabolism
MH  - MicroRNAs/*genetics
MH  - Oncogene Proteins, Viral/genetics/*metabolism
MH  - Proto-Oncogene Proteins c-fos/genetics/*metabolism
MH  - RNA, Messenger/genetics
MH  - Real-Time Polymerase Chain Reaction
MH  - Repressor Proteins/genetics/*metabolism
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Tumor Cells, Cultured
MH  - Up-Regulation
MH  - Uterine Cervical Neoplasms/genetics/*metabolism/pathology
EDAT- 2015/05/20 06:00
MHDA- 2016/02/18 06:00
CRDT- 2015/05/20 06:00
PHST- 2015/05/20 06:00 [entrez]
PHST- 2015/05/20 06:00 [pubmed]
PHST- 2016/02/18 06:00 [medline]
AID - 10.7314/apjcp.2015.16.9.3961 [doi]
PST - ppublish
SO  - Asian Pac J Cancer Prev. 2015;16(9):3961-8. doi: 10.7314/apjcp.2015.16.9.3961.