PMID- 25989684
OWN - NLM
STAT- MEDLINE
DCOM- 20150901
LR  - 20190923
IS  - 1000-8713 (Print)
IS  - 1000-8713 (Linking)
VI  - 33
IP  - 2
DP  - 2015 Feb
TI  - [Multi-residue determination of veterinary drugs in cheese by QuEChERS and liquid 
      chromatography-tandem mass spectrometry].
PG  - 132-9
AB  - A rapid and sensitive method has been developed for the simultaneous 
      determination of 50 veterinary drugs (including macrolides, quinolones, 
      sulfonamides, and tetracyclines) in cheese using a modified QuEChERS method 
      coupled with liquid chromatography-tandem mass spectrometry (LC-MS/MS). After 
      adding Na2EDTA buffer solution and ceramic homogenizer, the cheese sample was 
      extracted by 5% (v/v) acetic acid-acetonitrile, sodium chloride and anhydrous 
      sodium sulfate were used for salting-out process. The resulting supernatant 
      solution was purified by C18 sorbent and the re-dissolved solution was analyzed 
      by LC-MS/MS under dynamic multi-reaction monitoring (dynamic MRM) mode via 
      positive electrospray ionization with a ZORBAX-SB-C18 column. The limits of 
      quantification (LOQ, S/N= 10) of the target compounds ranged from 0. 05 microg/kg to 
      20 microg/kg in cheese. At spiked levels of 20, 50, 100 microg/kg (n =6) , the 
      percentages of drug with a recovery between 70% and 120% were 94%, 92%, and 96%, 
      respectively. The relative standard deviations (RSDs) were between 1% and 14%. 
      This method has been applied to determine seven real samples from markets. 
      Roxithromycin and flumequine have been detected in two samples separately. The 
      results demonstrated that this simple, rapid and accurate method is suitable for 
      the detection of multi-class veterinary drugs in cheese.
FAU - Cao, Yafei
AU  - Cao Y
FAU - Kang, Jian
AU  - Kang J
FAU - Chang, Qiaoying
AU  - Chang Q
FAU - Hu, Xueyan
AU  - Hu X
FAU - Wang, Zhibin
AU  - Wang Z
FAU - Fan, Chunlin
AU  - Fan C
FAU - Wang, Minglin
AU  - Wang M
LA  - chi
PT  - Journal Article
PL  - China
TA  - Se Pu
JT  - Se pu = Chinese journal of chromatography
JID - 9424804
RN  - 0 (Anti-Bacterial Agents)
RN  - 0 (Macrolides)
RN  - 0 (Quinolones)
RN  - 0 (Sulfonamides)
RN  - 0 (Tetracyclines)
RN  - 0 (Veterinary Drugs)
SB  - IM
MH  - Anti-Bacterial Agents
MH  - Cheese/*analysis
MH  - Chromatography, Liquid
MH  - Drug Residues/*analysis
MH  - Food Contamination/*analysis
MH  - Macrolides
MH  - Quinolones
MH  - Sulfonamides
MH  - Tandem Mass Spectrometry
MH  - Tetracyclines
MH  - Veterinary Drugs/*analysis
EDAT- 2015/05/21 06:00
MHDA- 2015/09/02 06:00
CRDT- 2015/05/21 06:00
PHST- 2015/05/21 06:00 [entrez]
PHST- 2015/05/21 06:00 [pubmed]
PHST- 2015/09/02 06:00 [medline]
AID - 10.3724/sp.j.1123.2014.10022 [doi]
PST - ppublish
SO  - Se Pu. 2015 Feb;33(2):132-9. doi: 10.3724/sp.j.1123.2014.10022.