PMID- 25990271
OWN - NLM
STAT- MEDLINE
DCOM- 20160616
LR  - 20181113
IS  - 1543-706X (Electronic)
IS  - 1071-2690 (Linking)
VI  - 51
IP  - 8
DP  - 2015 Sep
TI  - Deoxycholic acid inhibited proliferation and induced apoptosis and necrosis by 
      regulating the activity of transcription factors in rat pancreatic acinar cell 
      line AR42J.
PG  - 851-6
LID - 10.1007/s11626-015-9907-x [doi]
AB  - The objective of this study is to investigate the effect of deoxycholic acid 
      (DCA) on rat pancreatic acinar cell line AR42J and the functional mechanisms of 
      DCA on AR42J cells. AR42J cells were treated with various concentrations of DCA 
      for 24 h and also treated with 0.4 mmol/L DCA for multiple times, and then, 
      3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay was 
      performed to detect the AR42J cell survival rate. Flow cytometric was used to 
      detect the cell apoptosis and necrosis in AR42J cells treated with 0.4 mmol/L and 
      0.8 mmol/L DCA. The cells treated with phosphate buffer saline (PBS) were served 
      as control. In addition, the DNA-binding activity assays of transcription factors 
      (TFs) in nuclear proteins of cells treated with DCA were determined using 
      Panomics Procarta Transcription Factor Assay Kit. The relative survival rates 
      were markedly decreased (P < 0.05) in a dose- and time-dependent manner. Compared 
      with control group, the cell apoptosis and necrosis ratio were both significantly 
      elevated in 0.4 mmol/L DCA and 0.8 mmol/L DCA groups (P < 0.01). A significant 
      increase (P < 0.05) in the activity of transcription factor 2 (ATF2), 
      interferon-stimulated response element (ISRE), NKX-2.5, androgen receptor (AR), 
      p53, and hypoxia-inducible factor-1 (HIF-1) was observed, and the activity of 
      peroxisome proliferator-activated receptor (PPAR), activator protein 1 (AP1), and 
      E2F1 was reduced (P < 0.05). In conclusion, DCA inhibited proliferation and 
      induced apoptosis and necrosis in AR42J cells. The expression changes of related 
      genes regulated by TFs might be the molecular mechanism of AR42J cell injury.
FAU - Zhang, Guixin
AU  - Zhang G
AD  - Department of General Surgery, First Affiliated Hospital of Dalian Medical 
      University, Zhongshan Road 222, Dalian, China, 116011. guixinzhanggxz@163.com.
FAU - Zhang, Jingwen
AU  - Zhang J
AD  - Department of General Surgery, First Affiliated Hospital of Dalian Medical 
      University, Zhongshan Road 222, Dalian, China, 116011. jingwenzhangjwz@163.com.
AD  - Dalian Medical University, Dalian, China, 116044. jingwenzhangjwz@163.com.
FAU - Shang, Dong
AU  - Shang D
AD  - Department of General Surgery, First Affiliated Hospital of Dalian Medical 
      University, Zhongshan Road 222, Dalian, China, 116011. dongshangds@yeah.net.
FAU - Qi, Bing
AU  - Qi B
AD  - Department of General Surgery, First Affiliated Hospital of Dalian Medical 
      University, Zhongshan Road 222, Dalian, China, 116011. bingqibq@126.com.
FAU - Chen, Hailong
AU  - Chen H
AD  - Department of General Surgery, First Affiliated Hospital of Dalian Medical 
      University, Zhongshan Road 222, Dalian, China, 116011. hailongchenhlc@163.com.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150520
PL  - Germany
TA  - In Vitro Cell Dev Biol Anim
JT  - In vitro cellular & developmental biology. Animal
JID - 9418515
RN  - 0 (Transcription Factors)
RN  - 005990WHZZ (Deoxycholic Acid)
SB  - IM
MH  - Acinar Cells/*drug effects/physiology
MH  - Animals
MH  - Apoptosis/*drug effects
MH  - Cell Line
MH  - Cell Proliferation/*drug effects
MH  - Deoxycholic Acid/*pharmacology
MH  - Flow Cytometry
MH  - Necrosis/chemically induced
MH  - Pancreas/*cytology/pathology/physiology
MH  - Rats
MH  - Transcription Factors/drug effects/*physiology
OTO - NOTNLM
OT  - Cell injury
OT  - Deoxycholic acid
OT  - Rat pancreatic acinar cell
OT  - Transcription factors
EDAT- 2015/05/21 06:00
MHDA- 2016/06/17 06:00
CRDT- 2015/05/21 06:00
PHST- 2014/10/26 00:00 [received]
PHST- 2015/04/01 00:00 [accepted]
PHST- 2015/05/21 06:00 [entrez]
PHST- 2015/05/21 06:00 [pubmed]
PHST- 2016/06/17 06:00 [medline]
AID - 10.1007/s11626-015-9907-x [pii]
AID - 10.1007/s11626-015-9907-x [doi]
PST - ppublish
SO  - In Vitro Cell Dev Biol Anim. 2015 Sep;51(8):851-6. doi: 
      10.1007/s11626-015-9907-x. Epub 2015 May 20.