PMID- 25990956
OWN - NLM
STAT- MEDLINE
DCOM- 20161107
LR  - 20161230
IS  - 1942-7611 (Electronic)
IS  - 1942-7603 (Linking)
VI  - 8
IP  - 2
DP  - 2016 Feb
TI  - Development of a high-speed MALDI-triple quadrupole mass spectrometric method for 
      the determination of 3,4-methylenedioxymethamphetamine (MDMA) in oral fluid.
PG  - 235-40
LID - 10.1002/dta.1810 [doi]
AB  - 3,4-Methylenedioxymethamphetamine (MDMA, ecstasy) is still a widely used illicit 
      designer drug and its detection in different matrices is of major importance for 
      forensic purposes (e.g. driving under the influence) as well as for workplace 
      drug testing or abstinence control. Established analytical methods for the 
      determination of MDMA are mainly employing high performance liquid chromatography 
      (HPLC) or gas chromatography (GC) coupled to mass spectrometric detection. Matrix 
      assisted laser desorption/ionization-triple quadrupole-tandem mass spectrometry 
      (MALDI-QqQ-MS/MS) is so far rarely used in forensics and offers an ultrafast 
      high-throughput platform. The Quantisal Oral Fluid Collection Device was used 
      for sample collection. After addition of the deuterated internal standard and a 
      carbonate buffer (0.75 M Na2 CO3 ), oral fluid samples were liquid-liquid 
      extracted (ButOAc/EtOAc, 1:1). As little as 1 microlitre of a mixture of this 
      extract and the MALDI matrix (alpha-cyano-4-hydroxycinnamic acid) was spotted 
      onto the MALDI plate and could directly be analyzed. With MALDI omitting 
      chromatographic separation, very short analysis times of about 10 s per sample 
      were possible. The method was developed and validated according to international 
      guidelines including specificity, recovery, matrix effects, accuracy and 
      precision, stabilities and limit of quantification. All validation criteria were 
      fulfilled except for ion suppression/enhancement. Comparison with a routine 
      liquid chromatography-tandem mass spectrometry (LC-MS/MS) method showed good 
      agreement of the results. Applicability of the method was shown by analyzing 
      about 250 oral fluid samples collected after controlled administration of 125 mg 
      MDMA in a pharmacokinetic study. The whole lot of samples could be analyzed in 
      less than 1 h, proving the ultra-high-speed of the method.
CI  - Copyright (c) 2015 John Wiley & Sons, Ltd.
FAU - Poetzsch, Michael
AU  - Poetzsch M
AD  - Department of Forensic Pharmacology and Toxicology, ZIFM - Zurich Institute of 
      Forensic Medicine, University of Zurich, Zurich, Switzerland.
FAU - Steuer, Andrea E
AU  - Steuer AE
AD  - Department of Forensic Pharmacology and Toxicology, ZIFM - Zurich Institute of 
      Forensic Medicine, University of Zurich, Zurich, Switzerland.
FAU - Hysek, Cedric M
AU  - Hysek CM
AD  - Departement of Biomedicine, Division of Clinical Pharmacology and Toxicology, 
      University Hospital Basel, University of Basel, Basel, Switzerland.
FAU - Liechti, Matthias E
AU  - Liechti ME
AD  - Departement of Biomedicine, Division of Clinical Pharmacology and Toxicology, 
      University Hospital Basel, University of Basel, Basel, Switzerland.
FAU - Kraemer, Thomas
AU  - Kraemer T
AD  - Department of Forensic Pharmacology and Toxicology, ZIFM - Zurich Institute of 
      Forensic Medicine, University of Zurich, Zurich, Switzerland.
LA  - eng
PT  - Journal Article
DEP - 20150519
PL  - England
TA  - Drug Test Anal
JT  - Drug testing and analysis
JID - 101483449
RN  - 0 (Hallucinogens)
RN  - KE1SEN21RM (N-Methyl-3,4-methylenedioxyamphetamine)
SB  - IM
MH  - Body Fluids/chemistry
MH  - Calibration
MH  - Chromatography, High Pressure Liquid
MH  - Forensic Toxicology
MH  - Gas Chromatography-Mass Spectrometry
MH  - Hallucinogens/*analysis
MH  - Humans
MH  - Liquid-Liquid Extraction
MH  - Mouth
MH  - N-Methyl-3,4-methylenedioxyamphetamine/*analysis
MH  - Quality Control
MH  - Reproducibility of Results
MH  - Saliva/chemistry
MH  - Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
MH  - Tandem Mass Spectrometry
OTO - NOTNLM
OT  - MALDI
OT  - MDMA
OT  - ecstasy
OT  - high-speed analysis
OT  - oral fluid
EDAT- 2015/05/21 06:00
MHDA- 2016/11/08 06:00
CRDT- 2015/05/21 06:00
PHST- 2015/02/10 00:00 [received]
PHST- 2015/03/27 00:00 [revised]
PHST- 2015/04/07 00:00 [accepted]
PHST- 2015/05/21 06:00 [entrez]
PHST- 2015/05/21 06:00 [pubmed]
PHST- 2016/11/08 06:00 [medline]
AID - 10.1002/dta.1810 [doi]
PST - ppublish
SO  - Drug Test Anal. 2016 Feb;8(2):235-40. doi: 10.1002/dta.1810. Epub 2015 May 19.