PMID- 25997237
OWN - NLM
STAT- MEDLINE
DCOM- 20151124
LR  - 20150522
IS  - 1000-8020 (Print)
IS  - 1000-8020 (Linking)
VI  - 44
IP  - 2
DP  - 2015 Mar
TI  - [Determination of triclosan and triclocarban in human breast milk by solid-phase 
      extraction and ultra performance liquid chromatography-tandem mass spectrometry].
PG  - 294-8
AB  - OBJECTIVE: An analytical method was developed to simultaneously detect triclosan 
      (TCS) and triclocarban (TCC) in human breast milk using solid-phase extraction 
      (SPE) with ultra performance liquid chromatography-tandem mass spectrometry 
      (UPLC-MS/MS). METHODS: Samples were extracted by acetonitrile and purified with C 
      -18 SPE cartridge after enzymolysis with beta-glucuronidase/arylsulfatase. The 
      chromatographic separation was performed on a Waters ACQUITY UPLC HSS T3 column 
      (100 mm x 2. 1 mm, 1. 8 microm) with gradient elution using methanol and water at a 
      flow rate of 0. 3 ml/min. The target analytes were assayed by triple quadrupole 
      mass spectrometer operating in the negative ion mode. Quantification was 
      performed by isotopic internal standard calibration. RESULTS: Satisfactory 
      linearity (r2 > 0. 999) was obtained over the range of 0. 2 - 20. 0 microg/L and 0. 
      02 - 2. 0 microg/L for triclosan and triclocarban, respectively, with the limits of 
      quantifications (LOQs) of 0. 41 and 0. 03 microg/kg. Average recoveries of two target 
      compounds (spiked at three concentration levels) ranged from 100. 2% to 119. 3%, 
      with the relative standard deviations (RSDs) between 5. 91% and 11. 31% (n =6). 
      Twenty-five real samples (n = 25) were detected containing TCS and TCC at 
      concentrations of < LOQ - 0. 77 microg/kg and < LOQ - 4. 28 microg/kg, respectively. 
      CONCLUSION: Due to its high sensitivity and good reproductivity, this method can 
      be applied to analyze TCS and TCC in human breast milk.
FAU - Zhang, Pin
AU  - Zhang P
FAU - Zhang, Jing
AU  - Zhang J
FAU - Shi, Ying
AU  - Shi Y
FAU - Shao, Bing
AU  - Shao B
LA  - chi
PT  - English Abstract
PT  - Journal Article
PL  - China
TA  - Wei Sheng Yan Jiu
JT  - Wei sheng yan jiu = Journal of hygiene research
JID - 9426367
RN  - 0 (Carbanilides)
RN  - 4NM5039Y5X (Triclosan)
RN  - BGG1Y1ED0Y (triclocarban)
SB  - IM
MH  - Carbanilides/*analysis
MH  - Chromatography, High Pressure Liquid/*methods
MH  - Humans
MH  - Milk, Human/*chemistry
MH  - Reproducibility of Results
MH  - Sensitivity and Specificity
MH  - Solid Phase Extraction/*methods
MH  - Tandem Mass Spectrometry/*methods
MH  - Triclosan/*analysis
EDAT- 2015/05/23 06:00
MHDA- 2015/12/15 06:00
CRDT- 2015/05/23 06:00
PHST- 2015/05/23 06:00 [entrez]
PHST- 2015/05/23 06:00 [pubmed]
PHST- 2015/12/15 06:00 [medline]
PST - ppublish
SO  - Wei Sheng Yan Jiu. 2015 Mar;44(2):294-8.