PMID- 25997411
OWN - NLM
STAT- MEDLINE
DCOM- 20160324
LR  - 20211203
IS  - 1791-244X (Electronic)
IS  - 1107-3756 (Print)
IS  - 1107-3756 (Linking)
VI  - 36
IP  - 1
DP  - 2015 Jul
TI  - Gene set enrichment analysis of pathways and transcription factors associated 
      with diabetic retinopathy using a microarray dataset.
PG  - 103-12
LID - 10.3892/ijmm.2015.2220 [doi]
AB  - Diabetic retinopathy (DR) is a serious microvascular complication of diabetes, 
      which causes visual disability and blindness. Several studies have used gene 
      expression profiling of DR to identify the key genes involved in this process; 
      however, few studies have focused on the associated pathways and transcription 
      factors (TFs), or on the co-expression patterns at the multiple pathways level. 
      In this study, we employed a microarray dataset from the public database library 
      of the Gene Expression Omnibus (GEO) associated with DR and applied gene set 
      enrichment analysis (GSEA) to this dataset and performed candidate TF selection. 
      As a result, 10 upregulated pathways, including the type I diabetes mellitus and 
      peroxisome proliferator-activated receptor (PPAR) signaling pathways, as well as 
      59 downregulated pathways, including the ErbB signaling pathway and the mammalian 
      target of rapamycin (mTOR) signaling pathway, were identified as DR‑related 
      pathways. The majority of these pathways have been previously identified, but 
      some were novel. Finally, co-expression networks of related pathways were 
      constructed using the significant core genes and TFs, such as PPARgamma and SMAD4. 
      The results of our study may enhance our understanding of the molecular 
      mechanisms associated DR at the genome-wide level.
FAU - He, Kan
AU  - He K
AD  - Center for Stem Cell and Translational Medicine, School of Life Sciences, Anhui 
      University, Hefei, Anhui 230601, P.R. China.
FAU - Lv, Wenwen
AU  - Lv W
AD  - Center for Stem Cell and Translational Medicine, School of Life Sciences, Anhui 
      University, Hefei, Anhui 230601, P.R. China.
FAU - Zhang, Qing
AU  - Zhang Q
AD  - Department of Ophthalmology, The Second Hospital of Anhui Medical University, 
      Hefei, Anhui 230601, P.R. China.
FAU - Wang, Yuqing
AU  - Wang Y
AD  - Center for Stem Cell and Translational Medicine, School of Life Sciences, Anhui 
      University, Hefei, Anhui 230601, P.R. China.
FAU - Tao, Liming
AU  - Tao L
AD  - Department of Ophthalmology, The Second Hospital of Anhui Medical University, 
      Hefei, Anhui 230601, P.R. China.
FAU - Liu, Dahai
AU  - Liu D
AD  - Center for Stem Cell and Translational Medicine, School of Life Sciences, Anhui 
      University, Hefei, Anhui 230601, P.R. China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150522
PL  - Greece
TA  - Int J Mol Med
JT  - International journal of molecular medicine
JID - 9810955
RN  - 0 (PPAR gamma)
RN  - 0 (Peroxisome Proliferator-Activated Receptors)
RN  - 0 (SMAD4 protein, human)
RN  - 0 (Smad4 Protein)
RN  - 0 (Transcription Factors)
RN  - EC 2.7.1.1 (MTOR protein, human)
RN  - EC 2.7.10.1 (ErbB Receptors)
RN  - EC 2.7.11.1 (TOR Serine-Threonine Kinases)
SB  - IM
MH  - *Databases, Protein
MH  - Diabetes Mellitus, Type 1/complications/genetics/*pathology
MH  - Diabetic Retinopathy/metabolism/*pathology
MH  - ErbB Receptors/genetics/metabolism
MH  - Gene Expression Profiling
MH  - Gene Regulatory Networks/*genetics
MH  - Humans
MH  - Oligonucleotide Array Sequence Analysis
MH  - PPAR gamma/genetics/metabolism
MH  - Peroxisome Proliferator-Activated Receptors/genetics/metabolism
MH  - Signal Transduction/genetics
MH  - Smad4 Protein/genetics/metabolism
MH  - TOR Serine-Threonine Kinases/genetics/metabolism
MH  - Transcription Factors/genetics/*metabolism
PMC - PMC4494587
EDAT- 2015/05/23 06:00
MHDA- 2016/03/25 06:00
PMCR- 2015/05/22
CRDT- 2015/05/23 06:00
PHST- 2015/03/04 00:00 [received]
PHST- 2015/05/12 00:00 [accepted]
PHST- 2015/05/23 06:00 [entrez]
PHST- 2015/05/23 06:00 [pubmed]
PHST- 2016/03/25 06:00 [medline]
PHST- 2015/05/22 00:00 [pmc-release]
AID - ijmm-36-01-0103 [pii]
AID - 10.3892/ijmm.2015.2220 [doi]
PST - ppublish
SO  - Int J Mol Med. 2015 Jul;36(1):103-12. doi: 10.3892/ijmm.2015.2220. Epub 2015 May 
      22.