PMID- 26000275
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20150522
LR  - 20200930
IS  - 2296-634X (Print)
IS  - 2296-634X (Electronic)
IS  - 2296-634X (Linking)
VI  - 3
DP  - 2015
TI  - Pannexin channels mediate the acquisition of myogenic commitment in C2C12 reserve 
      cells promoted by P2 receptor activation.
PG  - 25
LID - 10.3389/fcell.2015.00025 [doi]
LID - 25
AB  - The acquisition of myoblast commitment to the myogenic linage requires rises in 
      intracellular free Ca(2+) concentration ([Ca(2+)]i). Putative cell membrane 
      pathways involved in these [Ca(2+)]i increments are P2 receptors (P2Rs) as well 
      as connexin (Cx) and/or pannexin (Panx) hemichannels and channels (Cx HChs and 
      Panx Chs), respectively, which are known to permeate Ca(2+). Reserve cells (RCs) 
      are uncommitted myoblasts obtained from differentiated C2C12 cell cultures, which 
      acquire commitment upon replating. Regarding these cells, we found that 
      extracellular ATP increases the [Ca(2+)]i via P2Rs. Moreover, ATP increases the 
      plasma membrane permeability to small molecules and a non-selective membrane 
      current, both of which were inhibited by Cx HCh/Panx1Ch blockers. However, RCs 
      exposed to divalent cation-free saline solution, which is known to activate Cx 
      HChs (but not Panx Chs), did not enhance membrane permeability, thus ruling out 
      the possible involvement of Cx HChs. Moreover, ATP-induced membrane permeability 
      was inhibited with blockers of P2Rs that activate Panx Chs. In addition, 
      exogenous ATP induced the expression of myogenic commitment and increased MyoD 
      levels, which was prevented by the inhibition of P2Rs or knockdown of Panx1 Chs. 
      Similarly, increases in MyoD levels induced by ATP released by RCs were inhibited 
      by Panx Ch/Cx HCh blockers. Myogenic commitment acquisition thus requires a 
      feed-forward mechanism mediated by extracellular ATP, P2Rs, and Panx Chs.
FAU - Riquelme, Manuel A
AU  - Riquelme MA
AD  - Departamento de Fisiologia, Facultad de Ciencias Biologicas, Pontificia 
      Universidad Catolica de Chile Santiago, Chile.
FAU - Cea, Luis A
AU  - Cea LA
AD  - Program of Anatomy and Developmental Biology, Institute of Biomedical Science, 
      Faculty of Medicine, University of Chile Santiago, Chile.
FAU - Vega, Jose L
AU  - Vega JL
AD  - Departamento de Fisiologia, Facultad de Ciencias Biologicas, Pontificia 
      Universidad Catolica de Chile Santiago, Chile ; Experimental Physiology 
      Laboratory (EPhyL), Instituto Antofagasta, Universidad de Antofagasta 
      Antofagasta, Chile.
FAU - Puebla, Carlos
AU  - Puebla C
AD  - Departamento de Fisiologia, Facultad de Ciencias Biologicas, Pontificia 
      Universidad Catolica de Chile Santiago, Chile.
FAU - Vargas, Anibal A
AU  - Vargas AA
AD  - Departamento de Fisiologia, Facultad de Ciencias Biologicas, Pontificia 
      Universidad Catolica de Chile Santiago, Chile.
FAU - Shoji, Kenji F
AU  - Shoji KF
AD  - Departamento de Fisiologia, Facultad de Ciencias Biologicas, Pontificia 
      Universidad Catolica de Chile Santiago, Chile.
FAU - Subiabre, Mario
AU  - Subiabre M
AD  - Departamento de Fisiologia, Facultad de Ciencias Biologicas, Pontificia 
      Universidad Catolica de Chile Santiago, Chile.
FAU - Saez, Juan C
AU  - Saez JC
AD  - Departamento de Fisiologia, Facultad de Ciencias Biologicas, Pontificia 
      Universidad Catolica de Chile Santiago, Chile ; Centro Interdisciplinario de 
      Neurociencias de Valparaiso, Instituto Milenio, Universidad de Valparaiso 
      Valparaiso, Chile.
LA  - eng
PT  - Journal Article
DEP - 20150506
PL  - Switzerland
TA  - Front Cell Dev Biol
JT  - Frontiers in cell and developmental biology
JID - 101630250
PMC - PMC4422085
OTO - NOTNLM
OT  - ATP
OT  - MyoD
OT  - calcium signal
OT  - membrane permeability
OT  - myogenesis
OT  - pannexons
OT  - purinergic receptors
EDAT- 2015/05/23 06:00
MHDA- 2015/05/23 06:01
PMCR- 2015/01/01
CRDT- 2015/05/23 06:00
PHST- 2015/03/12 00:00 [received]
PHST- 2015/04/17 00:00 [accepted]
PHST- 2015/05/23 06:00 [entrez]
PHST- 2015/05/23 06:00 [pubmed]
PHST- 2015/05/23 06:01 [medline]
PHST- 2015/01/01 00:00 [pmc-release]
AID - 10.3389/fcell.2015.00025 [doi]
PST - epublish
SO  - Front Cell Dev Biol. 2015 May 6;3:25. doi: 10.3389/fcell.2015.00025. eCollection 
      2015.