PMID- 26000400
OWN - NLM
STAT- MEDLINE
DCOM- 20150618
LR  - 20190923
IS  - 1550-7033 (Print)
IS  - 1550-7033 (Linking)
VI  - 10
IP  - 11
DP  - 2014 Nov
TI  - Cytotoxicity and cytokine release in rat hepatocytes, C3A cells and macrophages 
      exposed to gold nanoparticles--effect of biological dispersion media or corona.
PG  - 3416-29
AB  - The study aim was to investigate how gold nanoparticles (NPs) of different sizes 
      (20 and 100 nm) influence primary hepatocytes, the hepatocyte cell line C3A and 
      macrophage cytokine responses when dispersed in lung or blood relevant fluids. 
      Gold Au NPs induced cytotoxicity in primary hepatocytes at the highest dose of 66 
      mug/cm2, this effect was modified by the dispersant, the effect was greater with 
      lung lining fluid (LLF). Release of interleukin (IL)-6, Monocyte chemoattractant 
      protein-1 (MCP-1) and IL-1beta was enhanced by the Au NPs and the effects were 
      influenced by the particle size and dispersant. In medium, the smallest particle 
      size was most effective at inducing IL-6 release, while in LLF the largest 
      particles were most effective at inducing IL-6 release. Both 20 nm and 100 nm 
      particles enhanced MCP-1 and IL-1beta in the presence of LLF. The Au particles had 
      no cytotoxic effects nor did they stimulate the release of cytokines in the C3A 
      hepatoma cell line. The Au NPs had no significant impact on macrophage viability. 
      Particles induced IL-6 and TNF-alpha release. LLF and serum reduced the IL-6 response 
      while albumin enhanced the TNF-alpha response compared to medium dispersed Au NPs. 
      The Au NPs did not impact on MCP-1 release, but this cytokine was enhanced by 
      albumin and serum, while it was depressed by LLF. The macrophage responses were 
      lower than those evoked in primary hepatocytes. In conclusion, when assessing the 
      cytotoxic and pro-inflammatory responses induced by Au NPs, the response is 
      influenced by the dispersant, with different dispersants having different effects 
      in different cell types.
FAU - Brown, David M
AU  - Brown DM
FAU - Johnston, Helinor
AU  - Johnston H
FAU - Gubbins, Eva
AU  - Gubbins E
FAU - Stone, Vicki
AU  - Stone V
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Biomed Nanotechnol
JT  - Journal of biomedical nanotechnology
JID - 101230869
RN  - 7440-57-5 (Gold)
SB  - IM
MH  - Animals
MH  - Cell Survival/drug effects/*immunology
MH  - Cells, Cultured
MH  - Gold/*toxicity
MH  - Hepatocytes/*drug effects/*immunology/pathology
MH  - Macrophages/*drug effects/*immunology/pathology
MH  - Male
MH  - Metal Nanoparticles/*toxicity
MH  - Particle Size
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Structure-Activity Relationship
EDAT- 2015/05/24 06:00
MHDA- 2015/06/19 06:00
CRDT- 2015/05/23 06:00
PHST- 2015/05/23 06:00 [entrez]
PHST- 2015/05/24 06:00 [pubmed]
PHST- 2015/06/19 06:00 [medline]
AID - 10.1166/jbn.2014.1871 [doi]
PST - ppublish
SO  - J Biomed Nanotechnol. 2014 Nov;10(11):3416-29. doi: 10.1166/jbn.2014.1871.