PMID- 26001774 OWN - NLM STAT- MEDLINE DCOM- 20151015 LR - 20200930 IS - 1522-1504 (Electronic) IS - 1040-0605 (Linking) VI - 309 IP - 3 DP - 2015 Aug 1 TI - ICAM-1-dependent and ICAM-1-independent neutrophil lung infiltration by porcine reproductive and respiratory syndrome virus infection. PG - L226-36 LID - 10.1152/ajplung.00037.2015 [doi] AB - Neutrophils are innate immune cells that play a crucial role in the first line of host defense. It is also known that neutrophil lung recruitment and infiltration may cause lung injury. The roles of neutrophils in virus infection-induced lung injury are not clear. We explore the mechanisms of neutrophil lung infiltration and the potential biomarkers for lung injury in a swine model of lung injury caused by natural or experimental porcine reproductive and respiratory syndrome virus (PRRSV) infection. Neutrophil lung infiltration was determined by measurement of myeloperoxidase expression and enzyme activity of lung tissues. Myeloperoxidase expression and enzyme activity were dramatically increased in the naturally and experimentally infected lung tissues. Chemokine analysis by quantitative PCR and ELISA showed that IL-8 expression was increased in both infections, while monocyte chemoattractant protein-1 expression was increased only in experimentally infected lung tissues. Expression of the cell adhesion molecules VCAM-1 and ICAM-1 was measured by quantitative PCR and Western blotting. VCAM-1 expression was increased in experimentally and naturally infected lungs, whereas ICAM-1 expression was increased only in the naturally infected lung samples. Our results suggest that neutrophil lung infiltrations in the infected animals are both ICAM-1- and -independent and that combined expression of VCAM-1 and IL-8 may serve as the biomarker for lung injury induced by virus infection. CI - Copyright (c) 2015 the American Physiological Society. FAU - Liu, Jie AU - Liu J AD - Department of Basic Veterinary Medicine, College of Animal Science and Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei Province, People's Republic of China; FAU - Hou, Make AU - Hou M AD - Department of Basic Veterinary Medicine, College of Animal Science and Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei Province, People's Republic of China; FAU - Yan, Meiping AU - Yan M AD - Department of Basic Veterinary Medicine, College of Animal Science and Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei Province, People's Republic of China; FAU - Lu, Xinhui AU - Lu X AD - Department of Basic Veterinary Medicine, College of Animal Science and Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei Province, People's Republic of China; FAU - Gu, Wei AU - Gu W AD - Department of Basic Veterinary Medicine, College of Animal Science and Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei Province, People's Republic of China; FAU - Zhang, Songlin AU - Zhang S AD - Shandong Binzhou Animal Science and Veterinary Medicine Academy, Binzhou, Shandong Province, People's Republic of China; FAU - Gao, Jianfeng AU - Gao J AD - Department of Clinical Veterinary Medicine, College of Animal Science and Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei Province, People's Republic of China; FAU - Liu, Bang AU - Liu B AD - Department of Animal Genetics and Breeding, College of Animal Science and Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei Province, People's Republic of China; and Key Lab of Swine Genetics and Breeding and Agricultural Animal Breeding and Reproduction, College of Animal Science and Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei Province, People's Republic of China. FAU - Wu, Xiaoxiong AU - Wu X AD - Department of Basic Veterinary Medicine, College of Animal Science and Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei Province, People's Republic of China; FAU - Liu, Guoquan AU - Liu G AD - Department of Basic Veterinary Medicine, College of Animal Science and Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei Province, People's Republic of China; Key Lab of Swine Genetics and Breeding and Agricultural Animal Breeding and Reproduction, College of Animal Science and Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei Province, People's Republic of China liuguoquan@mail.hzau.edu.cn. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20150522 PL - United States TA - Am J Physiol Lung Cell Mol Physiol JT - American journal of physiology. Lung cellular and molecular physiology JID - 100901229 RN - 0 (Chemokine CCL2) RN - 0 (Interleukin-8) RN - 126547-89-5 (Intercellular Adhesion Molecule-1) RN - EC 1.11.1.7 (Peroxidase) SB - IM MH - Animals MH - Chemokine CCL2/genetics/metabolism MH - Gene Expression MH - Intercellular Adhesion Molecule-1/*physiology MH - Interleukin-8/genetics/metabolism MH - Lung/*immunology/metabolism/virology MH - *Neutrophil Infiltration MH - Peroxidase/genetics/metabolism MH - Porcine Reproductive and Respiratory Syndrome/immunology/*metabolism/virology MH - Porcine respiratory and reproductive syndrome virus/*immunology MH - Sus scrofa MH - Swine MH - Up-Regulation OTO - NOTNLM OT - intercellular adhesion molecule 1 OT - lung injury OT - neutrophil EDAT- 2015/05/24 06:00 MHDA- 2015/10/16 06:00 CRDT- 2015/05/24 06:00 PHST- 2015/02/02 00:00 [received] PHST- 2015/05/13 00:00 [accepted] PHST- 2015/05/24 06:00 [entrez] PHST- 2015/05/24 06:00 [pubmed] PHST- 2015/10/16 06:00 [medline] AID - ajplung.00037.2015 [pii] AID - 10.1152/ajplung.00037.2015 [doi] PST - ppublish SO - Am J Physiol Lung Cell Mol Physiol. 2015 Aug 1;309(3):L226-36. doi: 10.1152/ajplung.00037.2015. Epub 2015 May 22.