PMID- 26001828
OWN - NLM
STAT- MEDLINE
DCOM- 20151106
LR  - 20211203
IS  - 1551-5044 (Electronic)
IS  - 0022-1554 (Print)
IS  - 0022-1554 (Linking)
VI  - 63
IP  - 9
DP  - 2015 Sep
TI  - Silencing SOX2 Expression by RNA Interference Inhibits Proliferation, Invasion 
      and Metastasis, and Induces Apoptosis through MAP4K4/JNK Signaling Pathway in 
      Human Laryngeal Cancer TU212 Cells.
PG  - 721-33
LID - 10.1369/0022155415590829 [doi]
AB  - SRY (sex determining region Y)-box 2 (SOX2) plays an important role in tumor cell 
      metastasis and apoptosis. Laryngeal squamous cell carcinoma (LSCC), responsible 
      for 1.5% of all cancers, is one of the most common head and neck malignancies. 
      Accumulating evidence shows that SOX2 is overexpressed in several human tumors, 
      including lung cancer, esophageal carcinoma, pancreatic carcinoma, breast cancer, 
      ovarian carcinoma and glioma. Our study aimed to investigate the silencing 
      effects of SOX2 expression using RNA interference (RNAi) on various biological 
      processes in laryngeal cancer TU212 cells, including proliferation, apoptosis, 
      invasion and metastasis. We also studied the involvement of the MAPK/JNK 
      signaling pathway in the biological effects of SOX2 siRNA in TU212 cells. We 
      found that silencing SOX2 decreased the proliferation, migration, and invasion of 
      TU212 cells, and induced apoptosis. This effect of silencing SOX2 could be 
      reversed by silencing MAP4K4. Therefore, we consider SOX2 as a key regulator of 
      the upstream MAP4K4/JNK signaling pathways that could be a potential therapeutic 
      target in the treatment of patients with or prevention of laryngeal cancer.
CI  - (c) The Author(s) 2015.
FAU - Yang, Ning
AU  - Yang N
AD  - Department of Otorhinolaryngology, The First Affiliated Hospital of China Medical 
      University, Shenyang, People's Republic of China (NY, YW, LH, XL, XJ)
FAU - Wang, Yan
AU  - Wang Y
AD  - Department of Otorhinolaryngology, The First Affiliated Hospital of China Medical 
      University, Shenyang, People's Republic of China (NY, YW, LH, XL, XJ)
FAU - Hui, Lian
AU  - Hui L
AD  - Department of Otorhinolaryngology, The First Affiliated Hospital of China Medical 
      University, Shenyang, People's Republic of China (NY, YW, LH, XL, XJ)
FAU - Li, Xiaotian
AU  - Li X
AD  - Department of Otorhinolaryngology, The First Affiliated Hospital of China Medical 
      University, Shenyang, People's Republic of China (NY, YW, LH, XL, XJ)
FAU - Jiang, Xuejun
AU  - Jiang X
AD  - Department of Otorhinolaryngology, The First Affiliated Hospital of China Medical 
      University, Shenyang, People's Republic of China (NY, YW, LH, XL, XJ)
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150522
PL  - United States
TA  - J Histochem Cytochem
JT  - The journal of histochemistry and cytochemistry : official journal of the 
      Histochemistry Society
JID - 9815334
RN  - 0 (Intracellular Signaling Peptides and Proteins)
RN  - 0 (RNA, Small Interfering)
RN  - 0 (SOXB1 Transcription Factors)
RN  - EC 2.7.1.11 (MAP4K4 protein, human)
RN  - EC 2.7.11.1 (Protein Serine-Threonine Kinases)
RN  - EC 2.7.11.24 (JNK Mitogen-Activated Protein Kinases)
SB  - IM
MH  - Apoptosis/*genetics
MH  - Carcinoma, Squamous Cell/genetics/pathology
MH  - Cell Line, Tumor
MH  - Cell Movement/genetics
MH  - Cell Proliferation/genetics
MH  - Clone Cells/pathology
MH  - Disease Progression
MH  - Down-Regulation/genetics
MH  - Enzyme Activation/genetics
MH  - Gene Expression Regulation, Neoplastic/genetics
MH  - Humans
MH  - Intracellular Signaling Peptides and Proteins/*metabolism
MH  - JNK Mitogen-Activated Protein Kinases/*metabolism
MH  - Laryngeal Neoplasms/genetics/*pathology
MH  - MAP Kinase Signaling System/*genetics
MH  - Neoplasm Invasiveness
MH  - Neoplasm Metastasis
MH  - Phosphorylation/genetics
MH  - Protein Serine-Threonine Kinases/*metabolism
MH  - *RNA Interference
MH  - RNA, Small Interfering/genetics
MH  - SOXB1 Transcription Factors/deficiency/*genetics
PMC - PMC4804729
OTO - NOTNLM
OT  - MAP4K4/JNK signaling pathways
OT  - SRY (sex determining region Y)-box 2 (SOX2)
OT  - apoptosis
OT  - invasion
OT  - laryngeal cancer TU212 cells
OT  - metastasis
OT  - proliferation
OT  - siRNA
COIS- Competing Interests: The authors declared no potential competing interests with 
      respect to the research, authorship, and/or publication of this article.
EDAT- 2015/05/24 06:00
MHDA- 2015/11/07 06:00
PMCR- 2016/09/01
CRDT- 2015/05/24 06:00
PHST- 2015/02/27 00:00 [received]
PHST- 2015/05/17 00:00 [accepted]
PHST- 2015/05/24 06:00 [entrez]
PHST- 2015/05/24 06:00 [pubmed]
PHST- 2015/11/07 06:00 [medline]
PHST- 2016/09/01 00:00 [pmc-release]
AID - 0022155415590829 [pii]
AID - 10.1369_0022155415590829 [pii]
AID - 10.1369/0022155415590829 [doi]
PST - ppublish
SO  - J Histochem Cytochem. 2015 Sep;63(9):721-33. doi: 10.1369/0022155415590829. Epub 
      2015 May 22.