PMID- 26002774
OWN - NLM
STAT- MEDLINE
DCOM- 20151228
LR  - 20150526
IS  - 1572-8781 (Electronic)
IS  - 1387-2176 (Linking)
VI  - 17
IP  - 3
DP  - 2015
TI  - A microfluidic cell culture device (muFCCD) to culture epithelial cells with 
      physiological and morphological properties that mimic those of the human 
      intestine.
PG  - 9966
LID - 10.1007/s10544-015-9966-5 [doi]
AB  - Physiological and morphological properties of the human intestine cannot be 
      accurately mimicked in conventional culture devices such as well plates and petri 
      dishes where intestinal epithelial cells form a monolayer with loose contacts 
      among cells. Here, we report a novel microfluidic cell culture device (muFCCD) 
      that can be used to culture cells as a human intestinal model. This device 
      enables intestinal epithelial cells (Caco-2) to grow three-dimensionally on a 
      porous membrane coated with fibronectin between two polydimethylsiloxane (PDMS) 
      layers. Within 3 days, Caco-2 cells cultured in the muFCCD formed villi- and 
      crypt-like structures with small intercellular spaces, while individual cells 
      were tightly connected to one another through the expression of the tight 
      junction protein occludin, and were covered with a secreted mucin, MUC-2. Caco-2 
      cells cultured in the muFCCD for 3 days were less susceptible to bacterial attack 
      than those cultured in transwell plates for 21 days. muFCCD-cultured Caco-2 cells 
      also displayed physiologically relevant absorption and paracellular transport 
      properties. These results suggest that our intestinal model more accurately 
      mimics the morphological and physiological properties of the intestine in vivo 
      than the conventional transwell culture model.
FAU - Chi, Meiying
AU  - Chi M
AD  - Department of Chemistry and Nano Sciences (BK21 plus), EwhaWomans University, 
      Seoul, 120-750, Korea.
FAU - Yi, Banya
AU  - Yi B
FAU - Oh, Seunghan
AU  - Oh S
FAU - Park, Dong-June
AU  - Park DJ
FAU - Sung, Jong Hwan
AU  - Sung JH
FAU - Park, Sungsu
AU  - Park S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Biomed Microdevices
JT  - Biomedical microdevices
JID - 100887374
SB  - IM
MH  - Batch Cell Culture Techniques/instrumentation
MH  - Biomimetic Materials/chemical synthesis
MH  - Caco-2 Cells
MH  - Cell Size
MH  - Cells, Cultured
MH  - Epithelial Cells/*cytology/*physiology
MH  - Equipment Design
MH  - Equipment Failure Analysis
MH  - Humans
MH  - Intestines/*cytology/*growth & development
MH  - *Lab-On-A-Chip Devices
MH  - Tissue Engineering/*instrumentation
MH  - Tissue Scaffolds
EDAT- 2015/05/24 06:00
MHDA- 2015/12/29 06:00
CRDT- 2015/05/24 06:00
PHST- 2015/05/24 06:00 [entrez]
PHST- 2015/05/24 06:00 [pubmed]
PHST- 2015/12/29 06:00 [medline]
AID - 10.1007/s10544-015-9966-5 [doi]
PST - ppublish
SO  - Biomed Microdevices. 2015;17(3):9966. doi: 10.1007/s10544-015-9966-5.