PMID- 26007333
OWN - NLM
STAT- MEDLINE
DCOM- 20160113
LR  - 20211203
IS  - 2072-6643 (Electronic)
IS  - 2072-6643 (Linking)
VI  - 7
IP  - 5
DP  - 2015 May 8
TI  - Leucine Promotes Proliferation and Differentiation of Primary Preterm Rat 
      Satellite Cells in Part through mTORC1 Signaling Pathway.
PG  - 3387-400
LID - 10.3390/nu7053387 [doi]
AB  - Signaling through the mammalian target of rapamycin (mTOR) in response to leucine 
      modulates many cellular and developmental processes. However, in the context of 
      satellite cell proliferation and differentiation, the role of leucine and mTORC1 
      is less known. This study investigates the role of leucine in the process of 
      proliferation and differentiation of primary preterm rat satellite cells, and the 
      relationship with mammalian target of rapamycin complex 1 (mTORC1) activation. 
      Dissociation of primary satellite cells occurred with type I collagenase and 
      trypsin, and purification, via different speed adherence methods. Satellite cells 
      with positive expression of Desmin were treated with leucine and rapamycin. We 
      observed that leucine promoted proliferation and differentiation of primary 
      satellite cells and increased the phosphorylation of mTOR. Rapamycin inhibited 
      proliferation and differentiation, as well as decreased the phosphorylation level 
      of mTOR. Furthermore, leucine increased the expression of MyoD and myogenin while 
      the protein level of MyoD decreased due to rapamycin. However, myogenin expressed 
      no affect by rapamycin. In conclusion, leucine may up-regulate the activation of 
      mTORC1 to promote proliferation and differentiation of primary preterm rat 
      satellite cells. We have shown that leucine promoted the differentiation of 
      myotubes in part through the mTORC1-MyoD signal pathway.
FAU - Dai, Jie-Min
AU  - Dai JM
AD  - Department of Pediatrics, The First Affiliated Hospital, Sun Yat-sen University, 
      Guangzhou, Guangdong Province 510080, China. daijm6639@foxmail.com.
FAU - Yu, Mu-Xue
AU  - Yu MX
AD  - Department of Pediatrics, The First Affiliated Hospital, Sun Yat-sen University, 
      Guangzhou, Guangdong Province 510080, China. yumuxue@mail.sysu.edu.cn.
FAU - Shen, Zhen-Yu
AU  - Shen ZY
AD  - Department of Pediatrics, The First Affiliated Hospital, Sun Yat-sen University, 
      Guangzhou, Guangdong Province 510080, China. shenzhy@mail.sysu.edu.cn.
FAU - Guo, Chu-Yi
AU  - Guo CY
AD  - Department of Pediatrics, The First Affiliated Hospital, Sun Yat-sen University, 
      Guangzhou, Guangdong Province 510080, China. guochuyi1990@foxmail.com.
FAU - Zhuang, Si-Qi
AU  - Zhuang SQ
AD  - Department of Pediatrics, The First Affiliated Hospital, Sun Yat-sen University, 
      Guangzhou, Guangdong Province 510080, China. zhuangsq@mail.sysu.edu.cn.
FAU - Qiu, Xiao-Shan
AU  - Qiu XS
AD  - Department of Pediatrics, The First Affiliated Hospital, Sun Yat-sen University, 
      Guangzhou, Guangdong Province 510080, China. qiuxsh@mail.sysu.edu.cn.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150508
PL  - Switzerland
TA  - Nutrients
JT  - Nutrients
JID - 101521595
RN  - 0 (Multiprotein Complexes)
RN  - 0 (MyoD Protein)
RN  - 0 (Myogenin)
RN  - EC 2.7.11.1 (Mechanistic Target of Rapamycin Complex 1)
RN  - EC 2.7.11.1 (TOR Serine-Threonine Kinases)
RN  - GMW67QNF9C (Leucine)
SB  - IM
MH  - Animals
MH  - Animals, Newborn
MH  - Cell Differentiation/*drug effects
MH  - Cell Proliferation/*drug effects
MH  - Leucine/*pharmacology
MH  - Mechanistic Target of Rapamycin Complex 1
MH  - Multiprotein Complexes/*metabolism
MH  - Muscle Fibers, Skeletal
MH  - Muscle, Skeletal/cytology/*drug effects
MH  - MyoD Protein/metabolism
MH  - Myogenin/metabolism
MH  - Phosphorylation
MH  - Premature Birth
MH  - Rats, Sprague-Dawley
MH  - Satellite Cells, Skeletal Muscle/*drug effects/physiology
MH  - Signal Transduction
MH  - TOR Serine-Threonine Kinases/*metabolism
MH  - Up-Regulation
PMC - PMC4446757
OTO - NOTNLM
OT  - MyoD
OT  - differentiation
OT  - leucine
OT  - mTOR
OT  - myogenin
OT  - preterm
OT  - proliferation
OT  - satellite cells
EDAT- 2015/05/27 06:00
MHDA- 2016/01/14 06:00
PMCR- 2015/05/01
CRDT- 2015/05/27 06:00
PHST- 2015/03/02 00:00 [received]
PHST- 2015/04/28 00:00 [revised]
PHST- 2015/04/29 00:00 [accepted]
PHST- 2015/05/27 06:00 [entrez]
PHST- 2015/05/27 06:00 [pubmed]
PHST- 2016/01/14 06:00 [medline]
PHST- 2015/05/01 00:00 [pmc-release]
AID - nu7053387 [pii]
AID - nutrients-07-03387 [pii]
AID - 10.3390/nu7053387 [doi]
PST - epublish
SO  - Nutrients. 2015 May 8;7(5):3387-400. doi: 10.3390/nu7053387.