PMID- 26008114 OWN - NLM STAT- MEDLINE DCOM- 20170116 LR - 20220408 IS - 1878-3554 (Electronic) IS - 0099-2399 (Linking) VI - 41 IP - 7 DP - 2015 Jul TI - Influence of Irrigation Sequence on the Adhesion of Root Canal Sealers to Dentin: A Fourier Transform Infrared Spectroscopy and Push-out Bond Strength Analysis. PG - 1108-11 LID - S0099-2399(15)00107-7 [pii] LID - 10.1016/j.joen.2015.02.001 [doi] AB - INTRODUCTION: There is a lack of evidence on the chemical interaction between sealers and dentin. The influence of irrigation on the chemical interaction between root canal sealers and dentin was analyzed by using Fourier transform infrared spectroscopy (FTIRS) and measurement of dislocation resistance. METHODS: Single-rooted teeth (n = 120) were instrumented with 3% NaOCl as the irrigant and divided into 4 groups (n = 30) on the basis of irrigation protocol: group 1, 3% NaOCl, 17% EDTA, water; group 2, 17% EDTA, 3% NaOCl, water; group 3, 3% NaOCl, QMix, water; group 4, 3% NaOCl, water. Each group was divided into 3 subgroups (n = 10) on the basis of the root canal sealer: A, epoxy resin (AH Plus); B, silicone (RoekoSeal); C, calcium hydroxide (Sealapex). The dislocation resistance was assessed by using push-out bond strength test. The data were statistically analyzed by three-way analysis of variance and Holm-Sidak tests (P = .05). Dentin powder treated as per the conditioning protocols mentioned was mixed with the sealers and analyzed by FTIRS. RESULTS: A significant interaction was observed between irrigation protocol, type of sealer, and root segment (P < .001) for AH Plus but not for RoekoSeal and Sealapex (P > .05). AH Plus showed the highest bond strength (P < .05). FTIRS showed chemical bonding between AH Plus and dentinal collagen. In groups 2 and 4, no chemical bonding was observed. CONCLUSIONS: Bond strength of sealers is differentially affected by the irrigation protocol. The epoxy resin sealer AH Plus chemically bonds to dentinal collagen. This interaction is influenced by the irrigation protocols. CI - Copyright (c) 2015 American Association of Endodontists. Published by Elsevier Inc. All rights reserved. FAU - Neelakantan, Prasanna AU - Neelakantan P AD - Saveetha Dental College and Hospitals, Saveetha University, Chennai, India; Academic Center for Dentistry Amsterdam (ACTA), Amsterdam, The Netherlands. Electronic address: prasanna_neelakantan@yahoo.com. FAU - Sharma, Subash AU - Sharma S AD - Saveetha Dental College and Hospitals, Saveetha University, Chennai, India. FAU - Shemesh, Hagay AU - Shemesh H AD - Academic Center for Dentistry Amsterdam (ACTA), Amsterdam, The Netherlands. FAU - Wesselink, Paul R AU - Wesselink PR AD - Academic Center for Dentistry Amsterdam (ACTA), Amsterdam, The Netherlands. LA - eng PT - Journal Article DEP - 20150523 PL - United States TA - J Endod JT - Journal of endodontics JID - 7511484 RN - 0 (Root Canal Filling Materials) RN - 0 (Root Canal Irrigants) MH - Adhesiveness MH - *Dental Bonding MH - Dental Stress Analysis MH - Dentin/*chemistry MH - Humans MH - Materials Testing MH - Root Canal Filling Materials/*chemistry MH - Root Canal Irrigants/*administration & dosage MH - Root Canal Preparation/*methods MH - Spectroscopy, Fourier Transform Infrared OTO - NOTNLM OT - AH Plus OT - FTIR spectroscopy OT - bond strength OT - dislocation resistance EDAT- 2015/05/27 06:00 MHDA- 2017/01/17 06:00 CRDT- 2015/05/27 06:00 PHST- 2014/08/17 00:00 [received] PHST- 2015/01/26 00:00 [revised] PHST- 2015/02/03 00:00 [accepted] PHST- 2015/05/27 06:00 [entrez] PHST- 2015/05/27 06:00 [pubmed] PHST- 2017/01/17 06:00 [medline] AID - S0099-2399(15)00107-7 [pii] AID - 10.1016/j.joen.2015.02.001 [doi] PST - ppublish SO - J Endod. 2015 Jul;41(7):1108-11. doi: 10.1016/j.joen.2015.02.001. Epub 2015 May 23.