PMID- 26010356
OWN - NLM
STAT- MEDLINE
DCOM- 20160126
LR  - 20181113
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 10
IP  - 5
DP  - 2015
TI  - The application of fluorescence in situ hybridization in different ploidy levels 
      cross-breeding of lily.
PG  - e0126899
LID - 10.1371/journal.pone.0126899 [doi]
LID - e0126899
AB  - 21 crossing were conducted between Asiatic Lily with different ploidy levels, the 
      results showed that the interploidy hybridization between diploid and tetraploid 
      lilies was not as successful as intraploidy hybridization. Regardless of male 
      sterility, triploid lilies could be used as female parents in the hybridization 
      which the progenies were aneuploidy. 3xx4x crosses could be cultured more 
      successfully than 3xx2x crosses. 45S rDNA was mapped on the chromosomes of seven 
      Lilium species and their progenies using fluorescence in situ hybridization 
      (FISH). FISH revealed six to sixteen 45S rDNA gene loci, and normally the sites 
      were not in pairs. The asymmetry indexes of LA (Longiflorum hybrids x Asiatic 
      hybrids) hybrids was higher than Asiatic hybrids, the evolution degree was LA 
      hybrids > Asiatic hybrids. 45S rDNA distributed variably on chromosome 1-10 and 
      12 among Asiatic hybrids. Chromosome 1 had invariable sites of 45S rDNA in all 
      Asiatic hybrids, which could be considered as the characteristic of Asiatic 
      hybrids. LA hybrid 'Freya' had two sites of 45S rDNA on one homologous chromosome 
      5, and also it could be found in the progenies. The karyotype and fluorescence in 
      situ hybridization with 45S rDNA as probe were applied to identify the different 
      genotypes of 9 hybrids. Typical chromosomes with parental signal sites could be 
      observed in all the genotypes of hybrids, it was confirmed that all the hybrids 
      were true.
FAU - Wang, Qing
AU  - Wang Q
AD  - College of Landscape Architecture, Beijing Forestry University, Beijing, 100083, 
      China.
FAU - Wang, Jingmao
AU  - Wang J
AD  - College of Landscape Architecture, Beijing Forestry University, Beijing, 100083, 
      China.
FAU - Zhang, Yiying
AU  - Zhang Y
AD  - College of Landscape Architecture, Beijing Forestry University, Beijing, 100083, 
      China.
FAU - Zhang, Yue
AU  - Zhang Y
AD  - College of Landscape Architecture, Beijing Forestry University, Beijing, 100083, 
      China.
FAU - Xu, Shunchao
AU  - Xu S
AD  - College of Landscape Architecture, Beijing Forestry University, Beijing, 100083, 
      China.
FAU - Lu, Yingmin
AU  - Lu Y
AD  - College of Landscape Architecture, Beijing Forestry University, Beijing, 100083, 
      China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150526
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (DNA Probes)
RN  - 0 (DNA, Ribosomal)
SB  - IM
MH  - *Breeding
MH  - Chromosomes, Plant/genetics
MH  - Crosses, Genetic
MH  - DNA Probes/metabolism
MH  - DNA, Ribosomal/genetics
MH  - Hybridization, Genetic
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Karyotyping
MH  - Lilium/*genetics
MH  - *Ploidies
PMC - PMC4444012
COIS- Competing Interests: The authors have declared that no competing interests exist.
EDAT- 2015/05/27 06:00
MHDA- 2016/01/27 06:00
PMCR- 2015/05/26
CRDT- 2015/05/27 06:00
PHST- 2014/11/16 00:00 [received]
PHST- 2015/04/08 00:00 [accepted]
PHST- 2015/05/27 06:00 [entrez]
PHST- 2015/05/27 06:00 [pubmed]
PHST- 2016/01/27 06:00 [medline]
PHST- 2015/05/26 00:00 [pmc-release]
AID - PONE-D-14-51566 [pii]
AID - 10.1371/journal.pone.0126899 [doi]
PST - epublish
SO  - PLoS One. 2015 May 26;10(5):e0126899. doi: 10.1371/journal.pone.0126899. 
      eCollection 2015.