PMID- 26010864
OWN - NLM
STAT- MEDLINE
DCOM- 20160421
LR  - 20240326
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 10
IP  - 5
DP  - 2015
TI  - In Vivo Assessment of Cold Tolerance through Chlorophyll-a Fluorescence in 
      Transgenic Zoysiagrass Expressing Mutant Phytochrome A.
PG  - e0127200
LID - 10.1371/journal.pone.0127200 [doi]
LID - e0127200
AB  - Chlorophyll-a fluorescence analysis provides relevant information about the 
      physiology of plants growing under abiotic stress. In this study, we evaluated 
      the influence of cold stress on the photosynthetic machinery of transgenic 
      turfgrass, Zoysia japonica, expressing oat phytochrome A (PhyA) or a hyperactive 
      mutant phytochrome A (S599A) with post-translational phosphorylation blocked. 
      Biochemical analysis of zoysiagrass subjected to cold stress revealed reduced 
      levels of hydrogen peroxide, increased proline accumulation, and enhanced 
      specific activities of antioxidant enzymes compared to those of control plants. 
      Detailed analyses of the chlorophyll-a fluorescence data through the so-called 
      OJIP test exhibited a marked difference in the physiological status among 
      transgenic and control plants. Overall, these findings suggest an enhanced level 
      of cold tolerance in S599A zoysiagrass cultivars as reflected in the biochemical 
      and physiological analyses. Further, we propose that chlorophyll-a fluorescence 
      analysis using OJIP test is an efficient tool in determining the physiological 
      status of plants under cold stress conditions.
FAU - Gururani, Mayank Anand
AU  - Gururani MA
AD  - Subtropical Horticulture Research Institute, Faculty of Biotechnology, Jeju 
      National University, Jeju 690-756, South Korea; School of Biotechnology, Yeungnam 
      University, Gyeongsan, Gyeongbook 712-749, South Korea.
FAU - Venkatesh, Jelli
AU  - Venkatesh J
AD  - Department of Molecular Biotechnology, Konkuk University, Seoul 143-701, South 
      Korea.
FAU - Ganesan, Markkandan
AU  - Ganesan M
AD  - Subtropical Horticulture Research Institute, Faculty of Biotechnology, Jeju 
      National University, Jeju 690-756, South Korea; Department of Biological 
      Sciences, Presidency University, Kolkata 700073, West Bengal, India.
FAU - Strasser, Reto Jorg
AU  - Strasser RJ
AD  - Bioenergetics Laboratory, University of Geneva, Jussy, CH-1254, Geneva, 
      Switzerland.
FAU - Han, Yunjeong
AU  - Han Y
AD  - Department of Biotechnology and Kumho Life Science Laboratory, Chonnam National 
      University, Gwangju 500-757, South Korea.
FAU - Kim, Jeong-Il
AU  - Kim JI
AD  - Department of Biotechnology and Kumho Life Science Laboratory, Chonnam National 
      University, Gwangju 500-757, South Korea.
FAU - Lee, Hyo-Yeon
AU  - Lee HY
AD  - Subtropical Horticulture Research Institute, Faculty of Biotechnology, Jeju 
      National University, Jeju 690-756, South Korea.
FAU - Song, Pill-Soon
AU  - Song PS
AD  - Subtropical Horticulture Research Institute, Faculty of Biotechnology, Jeju 
      National University, Jeju 690-756, South Korea.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150526
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (Phytochrome A)
RN  - 1406-65-1 (Chlorophyll)
RN  - YF5Q9EJC8Y (Chlorophyll A)
SB  - IM
MH  - Amino Acid Substitution
MH  - Chlorophyll/genetics/*metabolism
MH  - Chlorophyll A
MH  - *Cold Temperature
MH  - Cold-Shock Response/*physiology
MH  - Fluorescence
MH  - Mutation, Missense
MH  - Phytochrome A/*biosynthesis/genetics
MH  - Plants, Genetically Modified/genetics/*metabolism
MH  - Poaceae/genetics/*metabolism
PMC - PMC4444231
COIS- Competing Interests: The authors have declared that no competing interests exist.
EDAT- 2015/05/27 06:00
MHDA- 2016/04/22 06:00
PMCR- 2015/05/26
CRDT- 2015/05/27 06:00
PHST- 2015/03/03 00:00 [received]
PHST- 2015/04/13 00:00 [accepted]
PHST- 2015/05/27 06:00 [entrez]
PHST- 2015/05/27 06:00 [pubmed]
PHST- 2016/04/22 06:00 [medline]
PHST- 2015/05/26 00:00 [pmc-release]
AID - PONE-D-15-09306 [pii]
AID - 10.1371/journal.pone.0127200 [doi]
PST - epublish
SO  - PLoS One. 2015 May 26;10(5):e0127200. doi: 10.1371/journal.pone.0127200. 
      eCollection 2015.