PMID- 26012218
OWN - NLM
STAT- MEDLINE
DCOM- 20150702
LR  - 20191113
IS  - 1110-0583 (Print)
IS  - 1110-0583 (Linking)
VI  - 45
IP  - 1
DP  - 2015 Apr
TI  - Studies on the role of tumor necrosis factor- alpha (TNF-alpha) in hepatocytes 
      induced apoptosis in vaccinated, Schistosoma mansoni-challenged mice.
PG  - 47-60
AB  - Tumour Necrosis Factor-alpha (TNF-alpha) plays a complex role in pathophysiological 
      changes caused by schistosomiasis in the liver cells as induced apoptosis. So, 
      The highlighted experimentally the role of TNF-alpha in hepatocytes apoptosis, using 
      that as an assessment of the efficacy of antischistosomal vaccination by mixed 
      crude antigens preparations [Cercarial antigen preparation (CAP) + soluble worm 
      antigen preparation (SWAP) + soluble egg antigen(SEA)] by parasitological, 
      histo-pathological and histochemical studies using Feulgen stain of hepatoytes 
      DNA, a serological study also of serum TNF-alpha level by ELISA. Fifty two laboratory 
      bred Albino male mice, were used in this study. They were classified into four 
      groups (13 mice in each group), G1: normal control, G2 as infected control while 
      G3 supported by Freund's Adjuvant (F. Adj) then infected and G4 vaccinated with 
      combined antigens (CAP, SWAP and SEA) + F. Adj, then infected. Mice were 
      sacrificed by cervical dislocation 9 weeks post infection, parasitological 
      (Kato-Katz thick smear for egg count), histopathologial haematoxylin and eosin 
      (H&E) staining of hepatic sections, histochemical (feulgen staining of 
      hepatocytes DNA) and ELISA to estimate serum TNF-alpha level were performed. The data 
      showed that vaccination with combined antigens showed protective effect on 
      vaccinated then Schistosoma challenged mice, hepatocytes induced apoptosis was 
      directly proportional with the TNF-alpha serum level, and the protection degree of 
      potential combined vaccine was inversely proportional with serum TNF-alpha level and 
      induced apoptosis.
FAU - Etewa, Samia E
AU  - Etewa SE
FAU - Abd El-Aal, Naglaa F
AU  - Abd El-Aal NF
FAU - Abdel-Rahman, Sara A
AU  - Abdel-Rahman SA
FAU - Abd El Bary, Eman H
AU  - Abd El Bary EH
FAU - El-Shafei, Mahmoud A
AU  - El-Shafei MA
LA  - eng
PT  - Journal Article
PL  - Egypt
TA  - J Egypt Soc Parasitol
JT  - Journal of the Egyptian Society of Parasitology
JID - 8102141
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 0 (Vaccines)
SB  - IM
MH  - Animals
MH  - Apoptosis/*physiology
MH  - Hepatocytes/immunology/*physiology
MH  - Male
MH  - Mice
MH  - Schistosoma mansoni/*immunology
MH  - Schistosomiasis mansoni/*prevention & control
MH  - Tumor Necrosis Factor-alpha/genetics/*metabolism
MH  - Vaccines/*immunology
EDAT- 2015/05/28 06:00
MHDA- 2015/07/03 06:00
CRDT- 2015/05/28 06:00
PHST- 2015/05/28 06:00 [entrez]
PHST- 2015/05/28 06:00 [pubmed]
PHST- 2015/07/03 06:00 [medline]
AID - 10.12816/0010849 [doi]
PST - ppublish
SO  - J Egypt Soc Parasitol. 2015 Apr;45(1):47-60. doi: 10.12816/0010849.