PMID- 26014706
OWN - NLM
STAT- MEDLINE
DCOM- 20150910
LR  - 20150527
IS  - 1008-8830 (Print)
IS  - 1008-8830 (Linking)
VI  - 17
IP  - 5
DP  - 2015 May
TI  - [Role of transient receptor potential melastatin 8 channels in migraine mechanism 
      in rats].
PG  - 515-9
AB  - OBJECTIVE: To investigate the role of transient receptor potential melastatin 8 
      (TRPM8) channels in migraine mechanism in rats by measuring the changes in 
      expression of TRPM8 in the trigeminal nerve of rats with migraine. METHODS: 
      Twenty male Sprague-Dawley rats were randomly and equally divided into a blank 
      control group and a model group. Nitroglycerin (10 mg/kg) was injected 
      subcutaneously in the back of the neck once a week for 5 weeks, to prepared a rat 
      model of migraine without aura. Normal saline was injected subcutaneously instead 
      of nitroglycerin in the control group. At 4 hours after the final injection, 
      behavior scoring of all rats was performed, and then the trigeminal nerve 
      ganglions of rats in both groups were collected for measurement of expression of 
      N-methyl-D-aspartate receptor (NMDAR), protein kinase A (PKA), and TRPM8 using 
      immunohistochemical staining, immunofluorescence, and Western blot, respectively. 
      RESULTS: The behavior score in each week during the rat model preparing was 
      significantly higher in the model group than in the control group (P<0.05). The 
      expression of NMDAR, PKA, and TRPM8 in the model group was significantly higher 
      than in the control group (P<0.01). Both the behavior score and the expression of 
      NMDAR were positively correlated with the expression of TRPM8 (r=0.822 and 0.794 
      respectively; P<0.01). CONCLUSIONS: TRPM8 may be involved in migraine mechanism 
      probably by activation of the NMDAR pathway.
FAU - Qin, Dong-Mei
AU  - Qin DM
AD  - Sichuan Academy of Medical Sciences and Sichuan Provincial People's Hospital, 
      Chengdu 610072, China. mufaguang@163.com.
FAU - Zou, Zhuan
AU  - Zou Z
FAU - Zhou, Chao-Ran
AU  - Zhou CR
FAU - Mu, Fa-Guang
AU  - Mu FG
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Zhongguo Dang Dai Er Ke Za Zhi
JT  - Zhongguo dang dai er ke za zhi = Chinese journal of contemporary pediatrics
JID - 100909956
RN  - 0 (Receptors, N-Methyl-D-Aspartate)
RN  - 0 (TRPM Cation Channels)
RN  - 0 (Trpm8 protein, rat)
RN  - EC 2.7.11.11 (Cyclic AMP-Dependent Protein Kinases)
SB  - IM
MH  - Animals
MH  - Cyclic AMP-Dependent Protein Kinases/analysis
MH  - Male
MH  - Migraine Disorders/*etiology
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Receptors, N-Methyl-D-Aspartate/analysis/physiology
MH  - TRPM Cation Channels/*physiology
EDAT- 2015/05/28 06:00
MHDA- 2015/09/12 06:00
CRDT- 2015/05/28 06:00
PHST- 2015/05/28 06:00 [entrez]
PHST- 2015/05/28 06:00 [pubmed]
PHST- 2015/09/12 06:00 [medline]
AID - 10.7499/j.issn.1008-8830.2015.05.020 [pii]
PST - ppublish
SO  - Zhongguo Dang Dai Er Ke Za Zhi. 2015 May;17(5):515-9.