PMID- 26015478
OWN - NLM
STAT- MEDLINE
DCOM- 20150924
LR  - 20181113
IS  - 1098-5522 (Electronic)
IS  - 0019-9567 (Print)
IS  - 0019-9567 (Linking)
VI  - 83
IP  - 8
DP  - 2015 Aug
TI  - Micropatterned macrophage analysis reveals global cytoskeleton constraints 
      induced by Bacillus anthracis edema toxin.
PG  - 3114-25
LID - 10.1128/IAI.00479-15 [doi]
AB  - Bacillus anthracis secretes the edema toxin (ET) that disrupts the cellular 
      physiology of endothelial and immune cells, ultimately affecting the adherens 
      junction integrity of blood vessels that in turn leads to edema. The effects of 
      ET on the cytoskeleton, which is critical in cell physiology, have not been 
      described thus far on macrophages. In this study, we have developed different 
      adhesive micropatterned surfaces (L and crossbow) to control the shape of bone 
      marrow-derived macrophages (BMDMs) and primary peritoneal macrophages. We found 
      that macrophage F-actin cytoskeleton adopts a specific polar organization 
      slightly different from classical human HeLa cells on the micropatterns. 
      Moreover, ET induced a major quantitative reorganization of F-actin within 16 h 
      with a collapse at the nonadhesive side of BMDMs along the nucleus. There was an 
      increase in size and deformation into a kidney-like shape, followed by a decrease 
      in size that correlates with a global cellular collapse. The collapse of F-actin 
      was correlated with a release of focal adhesion on the patterns and decreased 
      cell size. Finally, the cell nucleus was affected by actin reorganization. By 
      using this technology, we could describe many previously unknown macrophage 
      cellular dysfunctions induced by ET. This novel tool could be used to analyze 
      more broadly the effects of toxins and other virulence factors that target the 
      cytoskeleton.
CI  - Copyright (c) 2015, American Society for Microbiology. All Rights Reserved.
FAU - Trescos, Yannick
AU  - Trescos Y
AD  - Unite Interactions Hote-Agents pathogenes, Institut de Recherche Biomedicale des 
      Armees, Bretigny-sur-Orge, France Laboratoire Pathogenie des Toxi-Infections 
      Bacteriennes, Institut Pasteur, Paris, France Ecole du Val-de-Grace, Paris, 
      France.
FAU - Tessier, Emilie
AU  - Tessier E
AD  - Unite Interactions Hote-Agents pathogenes, Institut de Recherche Biomedicale des 
      Armees, Bretigny-sur-Orge, France.
FAU - Rougeaux, Clemence
AU  - Rougeaux C
AD  - Unite Interactions Hote-Agents pathogenes, Institut de Recherche Biomedicale des 
      Armees, Bretigny-sur-Orge, France Laboratoire Pathogenie des Toxi-Infections 
      Bacteriennes, Institut Pasteur, Paris, France.
FAU - Goossens, Pierre L
AU  - Goossens PL
AD  - Laboratoire Pathogenie des Toxi-Infections Bacteriennes, Institut Pasteur, Paris, 
      France.
FAU - Tournier, Jean-Nicolas
AU  - Tournier JN
AD  - Unite Interactions Hote-Agents pathogenes, Institut de Recherche Biomedicale des 
      Armees, Bretigny-sur-Orge, France Laboratoire Pathogenie des Toxi-Infections 
      Bacteriennes, Institut Pasteur, Paris, France Ecole du Val-de-Grace, Paris, 
      France jntournier.irba@gmail.com.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150526
PL  - United States
TA  - Infect Immun
JT  - Infection and immunity
JID - 0246127
RN  - 0 (Actins)
RN  - 0 (Antigens, Bacterial)
RN  - 0 (Bacterial Toxins)
RN  - 0 (anthrax toxin)
SB  - IM
MH  - Actins/metabolism
MH  - Animals
MH  - Anthrax/*metabolism/microbiology
MH  - Antigens, Bacterial/genetics/*metabolism
MH  - Bacillus anthracis/genetics/*metabolism
MH  - Bacterial Toxins/genetics/*metabolism
MH  - Cytoskeleton/*metabolism/microbiology
MH  - Female
MH  - Humans
MH  - Mice, Inbred C57BL
PMC - PMC4496603
EDAT- 2015/05/28 06:00
MHDA- 2015/09/25 06:00
PMCR- 2016/02/01
CRDT- 2015/05/28 06:00
PHST- 2015/04/17 00:00 [received]
PHST- 2015/05/16 00:00 [accepted]
PHST- 2015/05/28 06:00 [entrez]
PHST- 2015/05/28 06:00 [pubmed]
PHST- 2015/09/25 06:00 [medline]
PHST- 2016/02/01 00:00 [pmc-release]
AID - IAI.00479-15 [pii]
AID - 00479-15 [pii]
AID - 10.1128/IAI.00479-15 [doi]
PST - ppublish
SO  - Infect Immun. 2015 Aug;83(8):3114-25. doi: 10.1128/IAI.00479-15. Epub 2015 May 
      26.