PMID- 26015864
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20150527
LR  - 20220311
IS  - 2008-7802 (Print)
IS  - 2008-8213 (Electronic)
IS  - 2008-7802 (Linking)
VI  - 6
DP  - 2015
TI  - Mutation of kisspeptin 1 gene in children with precocious puberty in isfahan 
      city.
PG  - 41
LID - 10.4103/2008-7802.156839 [doi]
LID - 41
AB  - BACKGROUND: Considering the role of kisspeptin (KISS) in the process of puberty, 
      this study aimed to determine the mutation of KISS1 gene among a group of 
      patients with idiopathic central precocious puberty (ICPP). METHODS: In this case 
      control study, a group of children with diagnosed ICPP and a group of healthy 
      children were selected. Genomic DNA was extracted from peripheral blood of 
      selected population. After proving the quality and quantity of extracted DNA 
      samples by nano-drop instrument, PCR was performed using 3 set of primers to 
      amplify all coding exons and flanking intron region of Kiss1 gene. RESULTS: In 
      this study, 33 patients with idiopathic PP and 30 control age and sex matched 
      children were studied. Genetic analysis indicated that there was not any 
      polymorphism or mutation in studied participants of the control group. Among 
      patients with ICPP, 4 single nucleotide polymorphisms within the promoter and 
      coding regions of KISS1 gene were determined in 9 patients (5 boys and 4 girls). 
      Among them, the c.-148 T > A was novel variant. CONCLUSIONS: The results of the 
      current study identified one novel polymorphism and three reported polymorphism 
      in KISS gene among patients with ICPP. It is recommended to design further 
      studies for analysis other genes related to ICPP in accordance with more 
      complementary biochemical evaluations is recommended also.
FAU - Mazaheri, Ali
AU  - Mazaheri A
AD  - Department of Pediatrics Endocrinology, Isfahan Endocrine and Metabolism Research 
      Center, Child Growth and Development Research Center, Isfahan University of 
      Medical Sciences, Isfahan, Iran.
FAU - Hashemipour, Mahin
AU  - Hashemipour M
AD  - Department of Pediatrics Endocrinology, Isfahan Endocrine and Metabolism Research 
      Center, Child Growth and Development Research Center, Isfahan University of 
      Medical Sciences, Isfahan, Iran.
FAU - Salehi, Mansour
AU  - Salehi M
AD  - Department of Genetics, School of Medicine, Isfahan University of Medical 
      Sciences, Isfahan, Iran.
FAU - Behnam, Mehdieh
AU  - Behnam M
AD  - Department of Genetics, School of Medicine, Isfahan University of Medical 
      Sciences, Isfahan, Iran.
FAU - Hovsepian, Silva
AU  - Hovsepian S
AD  - Child Growth and Development Research Center, Research Institute for Primordial 
      Prevention of Non- Communicable Disease, Isfahan University of Medical Sciences, 
      Isfahan, Iran.
FAU - Hassanzadeh, Akbar
AU  - Hassanzadeh A
AD  - Department of Epidemiology and Biostatistics, School of Public Health, Isfahan 
      University of Medical Sciences Isfahan, Iran.
LA  - eng
PT  - Journal Article
DEP - 20150511
PL  - Iran
TA  - Int J Prev Med
JT  - International journal of preventive medicine
JID - 101535380
PMC - PMC4434494
OTO - NOTNLM
OT  - Central precocious puberty
OT  - kisspeptin gene
OT  - mutation
OT  - polymorphism
COIS- Conflict of Interest: None declared.
EDAT- 2015/05/28 06:00
MHDA- 2015/05/28 06:01
PMCR- 2015/05/11
CRDT- 2015/05/28 06:00
PHST- 2014/08/19 00:00 [received]
PHST- 2015/03/13 00:00 [accepted]
PHST- 2015/05/28 06:00 [entrez]
PHST- 2015/05/28 06:00 [pubmed]
PHST- 2015/05/28 06:01 [medline]
PHST- 2015/05/11 00:00 [pmc-release]
AID - IJPVM-6-41 [pii]
AID - 10.4103/2008-7802.156839 [doi]
PST - epublish
SO  - Int J Prev Med. 2015 May 11;6:41. doi: 10.4103/2008-7802.156839. eCollection 
      2015.