PMID- 26016416
OWN - NLM
STAT- MEDLINE
DCOM- 20161006
LR  - 20161230
IS  - 1522-7243 (Electronic)
IS  - 1522-7235 (Linking)
VI  - 31
IP  - 1
DP  - 2016 Feb
TI  - Interaction between tryptophan-Sm(III) complex and DNA with the use of a acridine 
      orange dye fluorophor probe.
PG  - 210-6
LID - 10.1002/bio.2947 [doi]
AB  - The interaction of the Trp-Sm(III) complex with herring sperm DNA (hs-DNA) was 
      investigated with the use of acridine orange (AO) dye as a spectral probe for 
      UV-vis spectrophotometry and fluorescence spectroscopy. The results showed that 
      the both the Trp-Sm(III) complex and the AO molecule could intercalate into the 
      double helix of the DNA. The Sm(III)-(Trp)3 complex was stabilized by 
      intercalation into the DNA with binding constants: K(Ө)25 degrees C  = 7.14 x 10(5) 
       L.mol(-1) and K(Ө) 37 degrees C  = 5.28 x 10(4)  L.mol(-1), and it could displace the AO 
      dye from the AO-DNA complex in a competitive reaction. Computation of the 
      thermodynamic functions demonstrates that Deltar Hm (Ө) is the primary driving power 
      of the interaction between the Sm(III)(Trp)3 complex and the DNA. The results 
      from Scatchard and viscometry methods suggested that the interaction mode between 
      the Sm(III)(Trp)3 complex and the hs-DNA is groove binding and weak intercalation 
      binding.
CI  - Copyright (c) 2015 John Wiley & Sons, Ltd.
FAU - Xiong, Xiao Li
AU  - Xiong XL
AD  - College of Chemistry and Materials Science, Sichuan Normal University, Chengdu, 
      610066, People's Republic of China.
FAU - Zhao, Na
AU  - Zhao N
AD  - Beijing City University Academic Affairs Office, Beijing, 100083, People's 
      Republic of China.
FAU - Wang, Xing Ming
AU  - Wang XM
AD  - Department of Chemistry, School Materials Science and Engineering, Southwest 
      University of Science and Technology, Sichuan, Mianyang, 621010, People's 
      Republic of China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150527
PL  - England
TA  - Luminescence
JT  - Luminescence : the journal of biological and chemical luminescence
JID - 100889025
RN  - 0 (Fluorescent Dyes)
RN  - 42OD65L39F (Samarium)
RN  - 8DUH1N11BX (Tryptophan)
RN  - 9007-49-2 (DNA)
RN  - F30N4O6XVV (Acridine Orange)
SB  - IM
MH  - Acridine Orange/*chemistry
MH  - Animals
MH  - DNA/*chemistry
MH  - Fishes
MH  - Fluorescent Dyes/*chemistry
MH  - Male
MH  - Samarium/*chemistry
MH  - Spectrometry, Fluorescence
MH  - Spectrophotometry, Ultraviolet
MH  - Spermatozoa/*chemistry
MH  - Thermodynamics
MH  - Tryptophan/*chemistry
OTO - NOTNLM
OT  - Sm(III)
OT  - herring sperm DNA
OT  - interaction
OT  - rare earth complexes
OT  - tryptophan
EDAT- 2015/05/29 06:00
MHDA- 2016/10/08 06:00
CRDT- 2015/05/29 06:00
PHST- 2014/10/25 00:00 [received]
PHST- 2015/03/12 00:00 [revised]
PHST- 2015/04/24 00:00 [accepted]
PHST- 2015/05/29 06:00 [entrez]
PHST- 2015/05/29 06:00 [pubmed]
PHST- 2016/10/08 06:00 [medline]
AID - 10.1002/bio.2947 [doi]
PST - ppublish
SO  - Luminescence. 2016 Feb;31(1):210-6. doi: 10.1002/bio.2947. Epub 2015 May 27.