PMID- 26018800
OWN - NLM
STAT- MEDLINE
DCOM- 20160728
LR  - 20150921
IS  - 1613-4133 (Electronic)
IS  - 1613-4125 (Linking)
VI  - 59
IP  - 9
DP  - 2015 Sep
TI  - omega3-polyunsaturated fatty acids suppress lipoprotein-associated phospholipase A2 
      expression in macrophages and animal models.
PG  - 1771-9
LID - 10.1002/mnfr.201500022 [doi]
AB  - SCOPE: omega3-polyunsaturated fatty acids (omega3-PUFAs) have beneficial effects on 
      cardiovascular function, and lipoprotein-associated phospholipase A2 (Lp-PLA2 ) 
      is associated with the risk of cardiovascular disease. Here, we investigated the 
      effects of omega3-PUFAs on Lp-PLA2 expression in vitro and in vivo and explored the 
      mechanisms involved. METHODS AND RESULTS: Human monocyticcells (THP-1) were 
      induced into macrophages in an in vitro model. omega3-PUFAs suppressed Lp-PLA2 
      expression; the suppression induced by docosahexaenoic acid (DHA) was related to 
      reduced inflammation. Tumor necrosis factor-alpha (TNF-alpha) was employed to stimulate 
      the phosphorylation of p38 mitogen-activated protein kinase (MAPK), nuclear 
      factor-kappaB (NF-kappaB) p65 and Lp-PLA2 expression in macrophages. The stimulation was 
      inhibited by DHA and the anti-inflammatory drug sodium salicylate. Moreover, the 
      stimulation of Lp-PLA2 expression by TNF-alpha could be suppressed by NF-kappaB and MAPK 
      pathway inhibitors. Then, chronic inflammation was induced in an in vivo mouse 
      model, resulting in an increase in Lp-PLA2 expression in peripheral blood 
      mononuclear cells (PBMCs) and arteries. This increase was suppressed by omega3-PUFAs. 
      Inhibition of Lp-PLA2 transcription in PBMCs was also observed in 
      omega3-PUFA-enriched swine. CONCLUSION: Our results demonstrate that the protective 
      effects of omega3-PUFAs against cardiovascular events may be related to the 
      suppression of Lp-PLA2 levels.
CI  - (c) 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
FAU - Li, Zhuang
AU  - Li Z
AD  - Jilin Provincial Key Laboratory of Animal Embryo Engineering, College of Animal 
      Sciences, Jilin University, Changchun, China.
FAU - Ren, Wenzhi
AU  - Ren W
AD  - Jilin Provincial Key Laboratory of Animal Embryo Engineering, College of Animal 
      Sciences, Jilin University, Changchun, China.
FAU - Han, Xiaolei
AU  - Han X
AD  - Jilin Provincial Key Laboratory of Animal Embryo Engineering, College of Animal 
      Sciences, Jilin University, Changchun, China.
FAU - Liu, Xingxing
AU  - Liu X
AD  - Jilin Provincial Key Laboratory of Animal Embryo Engineering, College of Animal 
      Sciences, Jilin University, Changchun, China.
FAU - Wang, Gangqi
AU  - Wang G
AD  - Jilin Provincial Key Laboratory of Animal Embryo Engineering, College of Animal 
      Sciences, Jilin University, Changchun, China.
FAU - Zhang, Mingjun
AU  - Zhang M
AD  - Jilin Provincial Key Laboratory of Animal Embryo Engineering, College of Animal 
      Sciences, Jilin University, Changchun, China.
FAU - Pang, Daxin
AU  - Pang D
AD  - Jilin Provincial Key Laboratory of Animal Embryo Engineering, College of Animal 
      Sciences, Jilin University, Changchun, China.
FAU - Ouyang, Hongsheng
AU  - Ouyang H
AD  - Jilin Provincial Key Laboratory of Animal Embryo Engineering, College of Animal 
      Sciences, Jilin University, Changchun, China.
FAU - Tang, Xiaochun
AU  - Tang X
AD  - Jilin Provincial Key Laboratory of Animal Embryo Engineering, College of Animal 
      Sciences, Jilin University, Changchun, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150618
PL  - Germany
TA  - Mol Nutr Food Res
JT  - Molecular nutrition & food research
JID - 101231818
RN  - 0 (Anti-Inflammatory Agents)
RN  - 0 (Fatty Acids, Omega-3)
RN  - 0 (NF-kappa B)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 25167-62-8 (Docosahexaenoic Acids)
RN  - EC 2.7.11.24 (p38 Mitogen-Activated Protein Kinases)
RN  - EC 3.1.1.47 (1-Alkyl-2-acetylglycerophosphocholine Esterase)
RN  - EC 3.1.1.47 (PLA2G7 protein, human)
SB  - IM
MH  - 1-Alkyl-2-acetylglycerophosphocholine Esterase/genetics/*metabolism
MH  - Animals
MH  - Anti-Inflammatory Agents/pharmacology
MH  - Cardiovascular Diseases/prevention & control
MH  - Cell Line
MH  - Disease Models, Animal
MH  - Docosahexaenoic Acids/pharmacology
MH  - Down-Regulation
MH  - Fatty Acids, Omega-3/*pharmacology
MH  - *Gene Expression Regulation
MH  - Humans
MH  - Leukocytes, Mononuclear/drug effects/metabolism
MH  - Macrophages/*drug effects/metabolism
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - NF-kappa B/genetics/metabolism
MH  - Phosphorylation
MH  - Tumor Necrosis Factor-alpha/metabolism
MH  - p38 Mitogen-Activated Protein Kinases/genetics/metabolism
OTO - NOTNLM
OT  - Cardiovascular disease
OT  - Inflammation
OT  - Lipoprotein associated phospholipase A2
OT  - Signaling pathways
OT  - omega3-polyunsaturated fatty acids
EDAT- 2015/05/29 06:00
MHDA- 2016/07/29 06:00
CRDT- 2015/05/29 06:00
PHST- 2015/01/09 00:00 [received]
PHST- 2015/04/25 00:00 [revised]
PHST- 2015/05/18 00:00 [accepted]
PHST- 2015/05/29 06:00 [entrez]
PHST- 2015/05/29 06:00 [pubmed]
PHST- 2016/07/29 06:00 [medline]
AID - 10.1002/mnfr.201500022 [doi]
PST - ppublish
SO  - Mol Nutr Food Res. 2015 Sep;59(9):1771-9. doi: 10.1002/mnfr.201500022. Epub 2015 
      Jun 18.