PMID- 26019035
OWN - NLM
STAT- MEDLINE
DCOM- 20160229
LR  - 20190919
IS  - 1665-2681 (Print)
IS  - 1665-2681 (Linking)
VI  - 14
IP  - 4
DP  - 2015 Jul-Aug
TI  - The nuclear receptor FXR, but not LXR, up-regulates bile acid transporter 
      expression in non-alcoholic fatty liver disease.
PG  - 487-93
AB  - BACKGROUND: Non-alcoholic fatty liver disease (NAFLD) is the most common cause of 
      chronic liver disease. Patients with non-alcoholic steatohepatitis (NASH) have 
      increased plasmatic and hepatic concentrations of bile acids (BA), suggesting 
      that they can be associated with the progression of the disease. Hepatic nuclear 
      receptors are known to modulate genes controlling BA metabolism; thus, in this 
      work we aimed to compare the expression of liver nuclear receptors -farnesoid X 
      (FXR), small heterodimer partner (SHP) and liver X alpha (LXRalpha) receptors- and BA 
      transporters -sodium+/taurocholate cotransporting polypeptide (NTCP) and bile 
      salt export pump (BSEP)- in liver biopsy samples of patients with simple 
      steatosis (SS) and NASH. MATERIAL AND METHODS: Forty patients with biopsy-proven 
      NALFD were enrolled between 2009 and 2012; liver biopsies were classified as SS 
      (N = 20) or NASH (N = 20) according to the NAFLD activity score. Gene expression 
      of nuclear FXR, LXRalpha, SHP, NTCP and BSEP was analyzed by real-time reverse 
      transcription polymerase chain reaction and protein level was quantified by 
      western blot. RESULTS: Gene expression of FXR, SHP, NTCP and BSEP was 
      significantly up-regulated in the NASH group in comparison with SS patients (P < 
      0.05). In contrast, protein level for FXR, SHP and NTCP was decreased in the NASH 
      patients vs. the SS group (P < 0.05). Gene and protein profile of LXRalpha did not 
      show differences between groups. CONCLUSIONS: The results suggest that liver 
      nuclear receptors (FXR and SHP) and BA transporters (NTCP and BSEP) are 
      associated with the progression of NAFLD.
FAU - Aguilar-Olivos, Nancy E
AU  - Aguilar-Olivos NE
AD  - Liver Research Unit, Medica Sur Clinic & Foundation, Mexico City, Mexico.
FAU - Carrillo-Cordova, Daniel
AU  - Carrillo-Cordova D
AD  - Liver Research Unit, Medica Sur Clinic & Foundation, Mexico City, Mexico.
FAU - Oria-Hernandez, Jesus
AU  - Oria-Hernandez J
AD  - Biochemistry and Genetics Lab, National Institute of Pediatrics, Ministry of 
      Health, Mexico City, Mexico.
FAU - Sanchez-Valle, Vicente
AU  - Sanchez-Valle V
AD  - Liver Research Unit, Medica Sur Clinic & Foundation, Mexico City, Mexico.
FAU - Ponciano-Rodriguez, Guadalupe
AU  - Ponciano-Rodriguez G
AD  - Public Health Department, Faculty of Medicine, National Autonomous University of 
      Mexico, Mexico City, Mexico.
FAU - Ramirez-Jaramillo, Manuel
AU  - Ramirez-Jaramillo M
AD  - Pathology Department, Medica Sur Clinic & Foundation, Mexico City, Mexico.
FAU - Chable-Montero, Fredy
AU  - Chable-Montero F
AD  - Pathology Department, Medica Sur Clinic & Foundation, Mexico City, Mexico.
FAU - Chavez-Tapia, Norberto C
AU  - Chavez-Tapia NC
AD  - Liver Research Unit, Medica Sur Clinic & Foundation, Mexico City, Mexico.
FAU - Uribe, Misael
AU  - Uribe M
AD  - Liver Research Unit, Medica Sur Clinic & Foundation, Mexico City, Mexico.
FAU - Mendez-Sanchez, Nahum
AU  - Mendez-Sanchez N
AD  - Liver Research Unit, Medica Sur Clinic & Foundation, Mexico City, Mexico.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Mexico
TA  - Ann Hepatol
JT  - Annals of hepatology
JID - 101155885
RN  - 0 (ABCB11 protein, human)
RN  - 0 (ATP Binding Cassette Transporter, Subfamily B, Member 11)
RN  - 0 (ATP-Binding Cassette Transporters)
RN  - 0 (Liver X Receptors)
RN  - 0 (NR1H3 protein, human)
RN  - 0 (Orphan Nuclear Receptors)
RN  - 0 (Receptors, Cytoplasmic and Nuclear)
RN  - 0 (SLC12A3 protein, human)
RN  - 0 (Solute Carrier Family 12, Member 3)
RN  - 0 (nuclear receptor subfamily 0, group B, member 2)
RN  - 0C5V0MRU6P (farnesoid X-activated receptor)
SB  - IM
MH  - ATP Binding Cassette Transporter, Subfamily B, Member 11
MH  - ATP-Binding Cassette Transporters/*analysis/genetics
MH  - Adult
MH  - Biopsy
MH  - Blotting, Western
MH  - Disease Progression
MH  - Female
MH  - Gene Expression Profiling/methods
MH  - Humans
MH  - Liver/*chemistry/pathology
MH  - Liver X Receptors
MH  - Male
MH  - Middle Aged
MH  - Non-alcoholic Fatty Liver Disease/diagnosis/genetics/*metabolism
MH  - Orphan Nuclear Receptors/*analysis/genetics
MH  - Real-Time Polymerase Chain Reaction
MH  - Receptors, Cytoplasmic and Nuclear/*analysis/genetics
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Severity of Illness Index
MH  - Solute Carrier Family 12, Member 3/analysis/genetics
MH  - Up-Regulation
EDAT- 2015/05/29 06:00
MHDA- 2016/03/02 06:00
CRDT- 2015/05/29 06:00
PHST- 2015/05/29 06:00 [entrez]
PHST- 2015/05/29 06:00 [pubmed]
PHST- 2016/03/02 06:00 [medline]
AID - S1665-2681(19)31170-6 [pii]
PST - ppublish
SO  - Ann Hepatol. 2015 Jul-Aug;14(4):487-93.