PMID- 26019373
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20150528
LR  - 20201001
IS  - 0044-5991 (Print)
IS  - 1347-5800 (Electronic)
IS  - 0044-5991 (Linking)
VI  - 48
IP  - 2
DP  - 2015 Apr 28
TI  - Localization of Rod Bipolar Cells in the Mammalian Retina Using an Antibody 
      Against the alpha1c L-type Ca(2+) Channel.
PG  - 47-52
LID - 10.1267/ahc.14049 [doi]
AB  - Bipolar cells transmit stimuli via graded changes in membrane potential and 
      neurotransmitter release is modulated by Ca(2+) influx through L-type Ca(2+) 
      channels. The purpose of this study was to determine whether the alpha1c subunit of 
      L-type voltage-gated Ca(2+) channel (alpha1c L-type Ca(2+) channel) colocalizes with 
      protein kinase C alpha (PKC-alpha), which labels rod bipolar cells. Retinal whole 
      mounts and vertical sections from mouse, hamster, rabbit, and dog were 
      immunolabeled with antibodies against PKC-alpha and alpha1c L-type Ca(2+) channel, using 
      fluorescein isothiocyanate (FITC) and Cy5 as visualizing agents. 
      PKC-alpha-immunoreactive cells were morphologically identical to rod bipolar cells as 
      previously reported. Their cell bodies were located within the inner nuclear 
      layer, dendritic processes branched into the outer plexiform layer, and axons 
      extended into the inner plexiform layer. Immunostaining showed that alpha1c L-type 
      Ca(2+) channel colocalized with PKC-alpha in rod bipolar cells. The identical 
      expression of PKC-alpha and alpha1c L-type Ca(2+) channel indicates that the alpha1c L-type 
      Ca(2+) channel has a specific role in rod bipolar cells, and the antibody against 
      the alpha1c L-type Ca(2+) channel may be a useful marker for studying the 
      distribution of rod bipolar cells in mouse, hamster, rabbit, and dog retinas.
FAU - Huh, Yu-Jin
AU  - Huh YJ
AD  - Department of Biology, School of Life Sciences, BK21 Plus KNU Creative 
      BioResearch Group, College of Natural Sciences, and Brain Science and Engineering 
      Institute, Kyungpook National University , Daegu, 702-701, South Korea.
FAU - Choi, Jae-Sik
AU  - Choi JS
AD  - Department of Biology, School of Life Sciences, BK21 Plus KNU Creative 
      BioResearch Group, College of Natural Sciences, and Brain Science and Engineering 
      Institute, Kyungpook National University , Daegu, 702-701, South Korea.
FAU - Jeon, Chang-Jin
AU  - Jeon CJ
AD  - Department of Biology, School of Life Sciences, BK21 Plus KNU Creative 
      BioResearch Group, College of Natural Sciences, and Brain Science and Engineering 
      Institute, Kyungpook National University , Daegu, 702-701, South Korea.
LA  - eng
PT  - Journal Article
DEP - 20150424
PL  - Japan
TA  - Acta Histochem Cytochem
JT  - Acta histochemica et cytochemica
JID - 0147110
PMC - PMC4427564
OTO - NOTNLM
OT  - L-type Ca2+ channel
OT  - immunocytochemistry
OT  - mammalian retina
OT  - protein kinase C
OT  - rod bipolar cell
EDAT- 2015/05/29 06:00
MHDA- 2015/05/29 06:01
PMCR- 2015/04/28
CRDT- 2015/05/29 06:00
PHST- 2014/10/08 00:00 [received]
PHST- 2015/03/16 00:00 [accepted]
PHST- 2015/05/29 06:00 [entrez]
PHST- 2015/05/29 06:00 [pubmed]
PHST- 2015/05/29 06:01 [medline]
PHST- 2015/04/28 00:00 [pmc-release]
AID - JST.JSTAGE/ahc/14049 [pii]
AID - 10.1267/ahc.14049 [doi]
PST - ppublish
SO  - Acta Histochem Cytochem. 2015 Apr 28;48(2):47-52. doi: 10.1267/ahc.14049. Epub 
      2015 Apr 24.