PMID- 26019805
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20150528
LR  - 20201001
IS  - 2008-3866 (Print)
IS  - 2008-3874 (Electronic)
IS  - 2008-3866 (Linking)
VI  - 18
IP  - 4
DP  - 2015 Apr
TI  - Modulation of IKKbeta/NF-kappaB and TGF-beta1/Smad via Fuzheng Huayu recipe involves in 
      prevention of nutritional steatohepatitis and fibrosis in mice.
PG  - 404-11
AB  - OBJECTIVES: Fuzheng Huayu recipe (FZHY) exerts significant protective effects 
      against liver fibrosis by strengthening the body's resistance and removing blood 
      stasis. However, the molecular mechanisms through which FZHY affects liver 
      fibrosis are still unclear. In this study, we examined the expression levels of 
      factors involved in the inhibitor kappaB kinase-beta (IKK-beta)/nuclear factor-kappaB (NF-kappaB) 
      and transforming growth factor beta 1 (TGF-beta1)/Smad signaling pathways to 
      elucidate whether FZHY could attenuate nutritional steatohepatitis and fibrosis 
      in mice. MATERIALS AND METHODS: C57BL/6J mice were fed with methionine-choline 
      deficient (MCD) diet for 8 weeks to induce fibrotic steatohepatitis. FZHY and/or 
      heme oxygenase-1 (HO-1) chemical inducer (hemin) were administered to mice. The 
      effects of FZHY alone and in combination with hemin were assessed by comparing 
      the severity of hepatic injury, activation of hepatic stellate cells (HSCs), and 
      the expression of oxidative stress, inflammation and fibrogenesis related genes. 
      RESULTS: Administration of FZHY, hemin and FZHY plus hemin significantly 
      ameliorated liver injury. Additionally, our analysis indicated that 
      administration of these agents significantly attenuated oxidative stress, 
      downregulated the expression of pro-inflammatory and pro-fibrotic genes, 
      including IKK-beta, NF-kappaB, monocyte chemoattractant protein-1 (MCP-1), alpha-smooth 
      muscle actin (alpha-SMA), TGF-beta1, Smad3 and Smad4, and upregulated the expression of 
      the antifibrogenic gene Smad7 (P< 0.001). CONCLUSION: FZHY-containing therapies 
      prevented nutritional steatohepatitis and fibrosis through modulating the 
      expression of factors associated with the IKKbeta/NF-kappaB and TGF-beta1/Smad signaling 
      pathways and oxidative stress related genes.
FAU - Wang, Rong-Qi
AU  - Wang RQ
AD  - Department of Traditional and Western Medical Hepatology, Third Hospital of Hebei 
      Medical University, Shijiazhuang, China.
FAU - Mi, Hong-Mei
AU  - Mi HM
AD  - Department of Traditional and Western Medical Hepatology, Third Hospital of Hebei 
      Medical University, Shijiazhuang, China.
FAU - Li, Hui
AU  - Li H
AD  - Department of Traditional and Western Medical Hepatology, Third Hospital of Hebei 
      Medical University, Shijiazhuang, China.
FAU - Zhao, Su-Xian
AU  - Zhao SX
AD  - Department of Traditional and Western Medical Hepatology, Third Hospital of Hebei 
      Medical University, Shijiazhuang, China.
FAU - Jia, Yan-Hong
AU  - Jia YH
AD  - Department of Traditional and Western Medical Hepatology, Third Hospital of Hebei 
      Medical University, Shijiazhuang, China.
FAU - Nan, Yue-Min
AU  - Nan YM
AD  - Department of Traditional and Western Medical Hepatology, Third Hospital of Hebei 
      Medical University, Shijiazhuang, China.
LA  - eng
PT  - Journal Article
PL  - Iran
TA  - Iran J Basic Med Sci
JT  - Iranian journal of basic medical sciences
JID - 101517966
PMC - PMC4439457
OTO - NOTNLM
OT  - FuzhengHuayu
OT  - Heme oxygenase-1
OT  - Hepatic fibrosis
OT  - IKK-beta/NF-kappaB signaling pathway
OT  - Non-alcoholic steatohepatitis
OT  - TGF-beta1/Smad signaling pathway
COIS- Conflict of interest The authors declared no conflict of interest.
EDAT- 2015/05/29 06:00
MHDA- 2015/05/29 06:01
PMCR- 2015/04/01
CRDT- 2015/05/29 06:00
PHST- 2014/06/30 00:00 [received]
PHST- 2014/11/24 00:00 [accepted]
PHST- 2015/05/29 06:00 [entrez]
PHST- 2015/05/29 06:00 [pubmed]
PHST- 2015/05/29 06:01 [medline]
PHST- 2015/04/01 00:00 [pmc-release]
AID - IJBMS-18-404 [pii]
PST - ppublish
SO  - Iran J Basic Med Sci. 2015 Apr;18(4):404-11.