PMID- 26020478 OWN - NLM STAT- MEDLINE DCOM- 20160229 LR - 20181202 IS - 1422-0067 (Electronic) IS - 1422-0067 (Linking) VI - 16 IP - 6 DP - 2015 May 25 TI - High-throughput screening for a moderately halophilic phenol-degrading strain and its salt tolerance response. PG - 11834-48 LID - 10.3390/ijms160611834 [doi] AB - A high-throughput screening system for moderately halophilic phenol-degrading bacteria from various habitats was developed to replace the conventional strain screening owing to its high efficiency. Bacterial enrichments were cultivated in 48 deep well microplates instead of shake flasks or tubes. Measurement of phenol concentrations was performed in 96-well microplates instead of using the conventional spectrophotometric method or high-performance liquid chromatography (HPLC). The high-throughput screening system was used to cultivate forty-three bacterial enrichments and gained a halophilic bacterial community E3 with the best phenol-degrading capability. Halomonas sp. strain 4-5 was isolated from the E3 community. Strain 4-5 was able to degrade more than 94% of the phenol (500 mg . L(-1) starting concentration) over a range of 3%-10% NaCl. Additionally, the strain accumulated the compatible solute, ectoine, with increasing salt concentrations. PCR detection of the functional genes suggested that the largest subunit of multicomponent phenol hydroxylase (LmPH) and catechol 1,2-dioxygenase (C12O) were active in the phenol degradation process. FAU - Lu, Zhi-Yan AU - Lu ZY AD - State Environmental Protection Key Laboratory of Environmental Risk Assessment and Control on Chemical Process, School of Resources and Environmental Engineering, East China University of Science and Technology, Shanghai 200237, China. lzy1009a@163.com. FAU - Guo, Xiao-Jue AU - Guo XJ AD - State Environmental Protection Key Laboratory of Environmental Risk Assessment and Control on Chemical Process, School of Resources and Environmental Engineering, East China University of Science and Technology, Shanghai 200237, China. foreverkimi1017@126.com. FAU - Li, Hui AU - Li H AD - State Environmental Protection Key Laboratory of Environmental Risk Assessment and Control on Chemical Process, School of Resources and Environmental Engineering, East China University of Science and Technology, Shanghai 200237, China. huili@ecust.edu.cn. FAU - Huang, Zhong-Zi AU - Huang ZZ AD - State Environmental Protection Key Laboratory of Environmental Risk Assessment and Control on Chemical Process, School of Resources and Environmental Engineering, East China University of Science and Technology, Shanghai 200237, China. z2cyj@163.com. AD - School of Chemical Engineering, Shanghai University of Engineering Science, Shanghai 201620, China. z2cyj@163.com. FAU - Lin, Kuang-Fei AU - Lin KF AD - State Environmental Protection Key Laboratory of Environmental Risk Assessment and Control on Chemical Process, School of Resources and Environmental Engineering, East China University of Science and Technology, Shanghai 200237, China. kflin@ecust.edu.cn. FAU - Liu, Yong-Di AU - Liu YD AD - State Environmental Protection Key Laboratory of Environmental Risk Assessment and Control on Chemical Process, School of Resources and Environmental Engineering, East China University of Science and Technology, Shanghai 200237, China. ydliu@ecust.edu.cn. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20150525 PL - Switzerland TA - Int J Mol Sci JT - International journal of molecular sciences JID - 101092791 RN - 0 (Amino Acids, Diamino) RN - 0 (Bacterial Proteins) RN - 0 (Phenols) RN - 7GXZ3858RY (ectoine) RN - EC 1.- (Mixed Function Oxygenases) RN - EC 1.13.11.1 (Catechol 1,2-Dioxygenase) RN - EC 1.14.13.7 (phenol 2-monooxygenase) SB - IM MH - Amino Acids, Diamino/metabolism MH - Bacterial Proteins/genetics MH - Biodegradation, Environmental MH - Catechol 1,2-Dioxygenase/genetics MH - Halomonadaceae/genetics/*isolation & purification/*physiology MH - High-Throughput Screening Assays/*methods MH - Mixed Function Oxygenases/genetics MH - Phenols/*metabolism MH - *Salt Tolerance PMC - PMC4490417 OTO - NOTNLM OT - high-throughput screening OT - moderately halophilic bacteria OT - phenol-degrading OT - salt tolerance EDAT- 2015/05/29 06:00 MHDA- 2016/03/02 06:00 PMCR- 2015/06/01 CRDT- 2015/05/29 06:00 PHST- 2015/02/09 00:00 [received] PHST- 2015/05/06 00:00 [accepted] PHST- 2015/05/29 06:00 [entrez] PHST- 2015/05/29 06:00 [pubmed] PHST- 2016/03/02 06:00 [medline] PHST- 2015/06/01 00:00 [pmc-release] AID - ijms160611834 [pii] AID - ijms-16-11834 [pii] AID - 10.3390/ijms160611834 [doi] PST - epublish SO - Int J Mol Sci. 2015 May 25;16(6):11834-48. doi: 10.3390/ijms160611834.