PMID- 26020947 OWN - NLM STAT- MEDLINE DCOM- 20160414 LR - 20221207 IS - 1932-6203 (Electronic) IS - 1932-6203 (Linking) VI - 10 IP - 5 DP - 2015 TI - Circulating MiRNAs of 'Asian Indian Phenotype' Identified in Subjects with Impaired Glucose Tolerance and Patients with Type 2 Diabetes. PG - e0128372 LID - 10.1371/journal.pone.0128372 [doi] LID - e0128372 AB - Several omics technologies are underway worldwide with an aim to unravel the pathophysiology of a complex phenotype such as type 2 diabetes mellitus (T2DM). While recent studies imply a clinically relevant and potential biomarker role of circulatory miRNAs in the etiology of T2DM, there is lack of data on this aspect in Indians--an ethnic population characterized to represent 'Asian Indian phenotype' known to be more prone to develop T2DM and cardiovascular disease than Europeans. We performed global serum miRNA profiling and the validation of candidate miRNAs by qRT-PCR in a cohort of subjects comprised of normal glucose tolerance (NGT), impaired glucose tolerance (IGT) and patients with T2DM. Our study revealed 4 differentially expressed miRNAs (miR-128, miR-130b-3p, miR-374a-5p, miR-423-5p) in subjects with IGT and T2DM patients compared to control subjects. They were positively or negatively correlated to cholesterol levels, HbA1C, HOMA-IR and fasting insulin. Interestingly, circulating level of miR-128 and miR-130b-3p were also altered in serum of diet-induced diabetic mice compared to control animals. Among the altered circulating miRNAs, miR-128 had never been described in previous studies/populations and appeared to be a 'New Lead' in Indians. It was positively correlated with cholesterol both in prediabetic subjects and in diet-induced diabetic mice, suggesting that its increased level might be associated with the development of dyslipedemia associated with T2DM. Our findings imply directionality towards biomarker potential of miRNAs in the prevention/diagnosis/treatment outcomes of diabetes. FAU - Prabu, Paramasivam AU - Prabu P AD - Madras Diabetes Research Foundation and Dr. Mohan's Diabetes Specialities Centre, WHO Collaborating Centre for Non-Communicable Diseases Prevention and Control & IDF Centre of Education, Gopalapuram, Chennai-600086, India. FAU - Rome, Sophie AU - Rome S AD - CarMeN Laboratory (INSERM 1060, INRA 1397, INSA), University of Lyon, Faculte de Medecine Lyon-Sud, Chemin du Grand Revoyet, 69600, Oullins, France. FAU - Sathishkumar, Chandrakumar AU - Sathishkumar C AD - Madras Diabetes Research Foundation and Dr. Mohan's Diabetes Specialities Centre, WHO Collaborating Centre for Non-Communicable Diseases Prevention and Control & IDF Centre of Education, Gopalapuram, Chennai-600086, India. FAU - Aravind, Sankaramoorthy AU - Aravind S AD - Madras Diabetes Research Foundation and Dr. Mohan's Diabetes Specialities Centre, WHO Collaborating Centre for Non-Communicable Diseases Prevention and Control & IDF Centre of Education, Gopalapuram, Chennai-600086, India. FAU - Mahalingam, Balakumar AU - Mahalingam B AD - Madras Diabetes Research Foundation and Dr. Mohan's Diabetes Specialities Centre, WHO Collaborating Centre for Non-Communicable Diseases Prevention and Control & IDF Centre of Education, Gopalapuram, Chennai-600086, India. FAU - Shanthirani, Coimbatore Subramanian AU - Shanthirani CS AD - Madras Diabetes Research Foundation and Dr. Mohan's Diabetes Specialities Centre, WHO Collaborating Centre for Non-Communicable Diseases Prevention and Control & IDF Centre of Education, Gopalapuram, Chennai-600086, India. FAU - Gastebois, Caroline AU - Gastebois C AD - CarMeN Laboratory (INSERM 1060, INRA 1397, INSA), University of Lyon, Faculte de Medecine Lyon-Sud, Chemin du Grand Revoyet, 69600, Oullins, France. FAU - Villard, Audrey AU - Villard A AD - CarMeN Laboratory (INSERM 1060, INRA 1397, INSA), University of Lyon, Faculte de Medecine Lyon-Sud, Chemin du Grand Revoyet, 69600, Oullins, France. FAU - Mohan, Viswanathan AU - Mohan V AD - Madras Diabetes Research Foundation and Dr. Mohan's Diabetes Specialities Centre, WHO Collaborating Centre for Non-Communicable Diseases Prevention and Control & IDF Centre of Education, Gopalapuram, Chennai-600086, India. FAU - Balasubramanyam, Muthuswamy AU - Balasubramanyam M AD - Madras Diabetes Research Foundation and Dr. Mohan's Diabetes Specialities Centre, WHO Collaborating Centre for Non-Communicable Diseases Prevention and Control & IDF Centre of Education, Gopalapuram, Chennai-600086, India. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20150528 PL - United States TA - PLoS One JT - PloS one JID - 101285081 RN - 0 (Biomarkers) RN - 0 (Blood Glucose) RN - 0 (Glycated Hemoglobin A) RN - 0 (Insulin) RN - 0 (MIRN128 microRNA, human) RN - 0 (MIRN130 microRNA, human) RN - 0 (MIRN374 microRNA 374, human) RN - 0 (MIRN423 microRNA, human) RN - 0 (MicroRNAs) RN - 0 (hemoglobin A1c protein, human) RN - 97C5T2UQ7J (Cholesterol) SB - IM MH - Adult MH - Animals MH - Biomarkers/blood MH - Blood Glucose/metabolism MH - Case-Control Studies MH - Cholesterol/blood MH - Diabetes Mellitus, Experimental/blood/genetics/pathology MH - Diabetes Mellitus, Type 2/*blood/ethnology/genetics/pathology MH - Ethnicity MH - Fasting MH - Female MH - Gene Expression Regulation MH - Glucose Intolerance MH - Glucose Tolerance Test MH - Glycated Hemoglobin/metabolism MH - Humans MH - Insulin/blood MH - Insulin Resistance MH - Male MH - Mice MH - Mice, Inbred C57BL MH - MicroRNAs/*blood/genetics MH - Middle Aged MH - *Phenotype MH - Prediabetic State/*blood/ethnology/genetics/pathology PMC - PMC4447457 COIS- Competing Interests: The authors have declared that no competing interests exist. EDAT- 2015/05/29 06:00 MHDA- 2016/04/15 06:00 PMCR- 2015/05/28 CRDT- 2015/05/29 06:00 PHST- 2015/02/14 00:00 [received] PHST- 2015/04/26 00:00 [accepted] PHST- 2015/05/29 06:00 [entrez] PHST- 2015/05/29 06:00 [pubmed] PHST- 2016/04/15 06:00 [medline] PHST- 2015/05/28 00:00 [pmc-release] AID - PONE-D-15-06615 [pii] AID - 10.1371/journal.pone.0128372 [doi] PST - epublish SO - PLoS One. 2015 May 28;10(5):e0128372. doi: 10.1371/journal.pone.0128372. eCollection 2015.