PMID- 26023716
OWN - NLM
STAT- MEDLINE
DCOM- 20160229
LR  - 20181202
IS  - 1422-0067 (Electronic)
IS  - 1422-0067 (Linking)
VI  - 16
IP  - 6
DP  - 2015 May 27
TI  - Human adipose-derived mesenchymal progenitor cells engraft into rabbit articular 
      cartilage.
PG  - 12076-91
LID - 10.3390/ijms160612076 [doi]
AB  - Mesenchymal stem cells (MSCs) are known to have the potential for articular 
      cartilage regeneration, and are suggested for the treatment of osteoarthritis 
      (OA). Here, we investigated whether intra-articular injection of xenogeneic human 
      adipose-derived mesenchymal progenitor cells (haMPCs) promoted articular 
      cartilage repair in rabbit OA model and engrafted into rabbit articular 
      cartilage. The haMPCs were cultured in vitro, and phenotypes and differentiation 
      characteristics of cells were evaluated. OA was induced surgically by anterior 
      cruciate ligament transection (ACLT) and medical meniscectomy of knee joints. At 
      six weeks following surgery, hyaluronic acid (HA) or haMPCs was injected into the 
      knee joints, the contralateral knee served as normal control. All animals were 
      sacrificed at the 16th week post-surgery. Assessments were carried out by 
      macroscopic examination, hematoxylin/eosin (HE) and Safranin-O/Fast green 
      stainings and immunohistochemistry. The data showed that haMPC treatment promoted 
      cartilage repair. Signals of human mitochondrial can be directly detected in 
      haMPC treated cartilage. The haMPCs expressed human leukocyte antigen I (HLA-I) 
      but not HLA-II-DR in vivo. These results suggest that intra-articular injection 
      of haMPCs promotes regeneration of articular cartilage in rabbit OA model, and 
      support the notion that MPCs are transplantable between HLA-incompatible 
      individuals.
FAU - Wang, Wen
AU  - Wang W
AD  - Cellular Biomedicine Group, Palo Alto, CA 94301, USA. 
      maxwell.wang@cellbiomedgroup.com.
FAU - He, Na
AU  - He N
AD  - Cellular Biomedicine Group, Palo Alto, CA 94301, USA. 
      elena.he@cellbiomedgroup.com.
FAU - Feng, Chenchen
AU  - Feng C
AD  - Cellular Biomedicine Group, Palo Alto, CA 94301, USA. 
      iris.feng@cellbiomedgroup.com.
FAU - Liu, Victor
AU  - Liu V
AD  - Cellular Biomedicine Group, Palo Alto, CA 94301, USA. 
      victor.liu@cellbiomedgroup.com.
FAU - Zhang, Luyi
AU  - Zhang L
AD  - Cellular Biomedicine Group, Palo Alto, CA 94301, USA. 
      lizzy.zhang@cellbiomedgroup.com.
FAU - Wang, Fei
AU  - Wang F
AD  - Cellular Biomedicine Group, Palo Alto, CA 94301, USA. 
      richard.wang@cellbiomedgroup.com.
FAU - He, Jiaping
AU  - He J
AD  - Cellular Biomedicine Group, Palo Alto, CA 94301, USA. 
      jeffrey.he@cellbiomedgroup.com.
FAU - Zhu, Tengfang
AU  - Zhu T
AD  - Department of Pathology, Shanghai Medical College, Fudan University, Shanghai 
      200433, China. zhutengfang@shmu.edu.cn.
FAU - Wang, Shuyang
AU  - Wang S
AD  - Department of Pathology, Shanghai Medical College, Fudan University, Shanghai 
      200433, China. wangshuyang@shmu.edu.cn.
FAU - Qiao, Weiwei
AU  - Qiao W
AD  - Animal facility, Shanghai Medical College, Fudan University, Shanghai 200433, 
      China. qiaoweiwei@shmu.edu.cn.
FAU - Li, Suke
AU  - Li S
AD  - Cellular Biomedicine Group, Palo Alto, CA 94301, USA. 
      maggie.li@cellbiomedgroup.com.
FAU - Zhou, Guangdong
AU  - Zhou G
AD  - Department of Plastic and Reconstructive Surgery, Shanghai 9th People's Hospital, 
      Shanghai Jiao Tong University School of Medicine, Shanghai 200011, China. 
      guangdongzhou@126.com.
FAU - Zhang, Li
AU  - Zhang L
AD  - Cellular Biomedicine Group, Palo Alto, CA 94301, USA. 
      lizzy.zhang@cellbiomedgroup.com.
FAU - Dai, Chengxiang
AU  - Dai C
AD  - Cellular Biomedicine Group, Palo Alto, CA 94301, USA. 
      chase.dai@cellbiomedgroup.com.
FAU - Cao, Wei
AU  - Cao W
AD  - Cellular Biomedicine Group, Palo Alto, CA 94301, USA. 
      william.cao@cellbiomedgroup.com.
AD  - Department of Histology and Embryology, Shanghai Medical College, Fudan 
      University, Shanghai 200433, China. william.cao@cellbiomedgroup.com.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150527
PL  - Switzerland
TA  - Int J Mol Sci
JT  - International journal of molecular sciences
JID - 101092791
RN  - 0 (Histocompatibility Antigens Class I)
RN  - 9004-61-9 (Hyaluronic Acid)
SB  - IM
MH  - Adipose Tissue/*cytology
MH  - Animals
MH  - Arthritis, Experimental/pathology/*therapy
MH  - Cells, Cultured
MH  - Histocompatibility
MH  - Histocompatibility Antigens Class I/metabolism
MH  - Humans
MH  - Hyaluronic Acid/administration & dosage
MH  - Injections, Intra-Articular
MH  - Mesenchymal Stem Cell Transplantation/*methods
MH  - Mesenchymal Stem Cells/metabolism/*physiology
MH  - Osteoarthritis, Knee/*therapy
MH  - Rabbits
PMC - PMC4490430
OTO - NOTNLM
OT  - human adipose-derived mesenchymal progenitor cells
OT  - human leukocyte antigen
OT  - mesenchymal stem cells
OT  - osteoarthritis
EDAT- 2015/05/30 06:00
MHDA- 2016/03/02 06:00
PMCR- 2015/06/01
CRDT- 2015/05/30 06:00
PHST- 2015/03/26 00:00 [received]
PHST- 2015/04/27 00:00 [revised]
PHST- 2015/05/07 00:00 [accepted]
PHST- 2015/05/30 06:00 [entrez]
PHST- 2015/05/30 06:00 [pubmed]
PHST- 2016/03/02 06:00 [medline]
PHST- 2015/06/01 00:00 [pmc-release]
AID - ijms160612076 [pii]
AID - ijms-16-12076 [pii]
AID - 10.3390/ijms160612076 [doi]
PST - epublish
SO  - Int J Mol Sci. 2015 May 27;16(6):12076-91. doi: 10.3390/ijms160612076.