PMID- 26025397
OWN - NLM
STAT- MEDLINE
DCOM- 20160331
LR  - 20181202
IS  - 1165-158X (Electronic)
IS  - 0145-5680 (Linking)
VI  - 61
IP  - 2
DP  - 2015 May 8
TI  - Importance of CD44 on umbilical cord mesenchymal stem cells for expansion of 
      hematopoietic cells.
PG  - 18-25
AB  - Human umbilical cord mesenchymal stem cells (hUCMSCs) have important functions on 
      the expansion of hematopoietic stem cells (HSCs) through providing the essential 
      microenvironment for hematopoiesis. In order to test whether CD44 on hUCMSCs 
      could have a key function for the ability of hUCMSCs to expand human HSCs, the 
      soluble anti—CD44 antibody was added to the co—cultures of hUCMSCs 
      and cord blood (CB) CD34+ cells, which blocked the ability of hUCMSCs to expand 
      CB CD34+ cells significantly. Long—term culture initiating cell 
      (LTC—IC) assay revealed that the ability of multipotent differentiation of 
      CB CD34+ cells co—cultured with CD44 knockdown hUCMSCs could only retain 
      lasting at most for 5 weeks in vitro. In vivo assay, based on non—obese 
      diabetic/severe combined immunodeficient disease (NOD/SCID) mice, revealed that 
      the hematopoietic reconstitution potential of CB CD34+ cells co—cultured 
      with CD44 knockdown hUCMSCs is significantly reduced. The hematopoietic 
      supporting ability of hUCMSCs in vivo and in vitro is reduced upon the knockdown 
      of CD44. CD44 has important functions on the ability of hUCMSCs to expand human 
      HSCs in the cell— extrinsic control.
FAU - Zhang, Y J
AU  - Zhang YJ
AD  - Tianjin Central Hospital of Gynecology Obstetrics Department of Anesthesiology 
      Tianjin China yanju_z2@126.com.
FAU - Yang, Y X
AU  - Yang YX
AD  - School of Basic Medical Sciences, Peking University Department of Medical 
      Genetics Beijing China.
FAU - Hu, M Y
AU  - Hu MY
AD  - School of Basic Medical Sciences, Peking University Department of Medical 
      Genetics Beijing China.
FAU - Ni, Q K
AU  - Ni QK
AD  - School of Basic Medical Sciences, Peking University Department of Medical 
      Genetics Beijing China.
FAU - Li, H G
AU  - Li HG
AD  - School of Medicine, Indiana University Department of Medical and Molecular 
      genetics USA.
FAU - Miao, Z C
AU  - Miao ZC
AD  - Beijing Vitalstar Biotechnology Co., Ltd. Beijing China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150508
PL  - France
TA  - Cell Mol Biol (Noisy-le-grand)
JT  - Cellular and molecular biology (Noisy-le-Grand, France)
JID - 9216789
RN  - 0 (Antigens, CD34)
RN  - 0 (CD44 protein, human)
RN  - 0 (Hyaluronan Receptors)
RN  - 0 (RNA, Small Interfering)
SB  - IM
MH  - Animals
MH  - Antigens, CD34/metabolism
MH  - Cell Differentiation/genetics
MH  - Cells, Cultured
MH  - Cellular Microenvironment/physiology
MH  - Fetal Blood/cytology
MH  - G1 Phase/physiology
MH  - Hematopoiesis/*genetics/physiology
MH  - Hematopoietic Stem Cells/*cytology
MH  - Humans
MH  - Hyaluronan Receptors/*genetics
MH  - Mesenchymal Stem Cells/*cytology
MH  - Mice
MH  - Mice, Inbred NOD
MH  - Mice, SCID
MH  - RNA Interference
MH  - RNA, Small Interfering
MH  - S Phase/physiology
MH  - Umbilical Cord/cytology
EDAT- 2015/05/31 06:00
MHDA- 2016/04/01 06:00
CRDT- 2015/05/31 06:00
PHST- 2015/03/20 00:00 [received]
PHST- 2015/04/17 00:00 [accepted]
PHST- 2015/05/31 06:00 [entrez]
PHST- 2015/05/31 06:00 [pubmed]
PHST- 2016/04/01 06:00 [medline]
PST - epublish
SO  - Cell Mol Biol (Noisy-le-grand). 2015 May 8;61(2):18-25.