PMID- 26025552
OWN - NLM
STAT- MEDLINE
DCOM- 20160421
LR  - 20231213
IS  - 1618-2650 (Electronic)
IS  - 1618-2642 (Linking)
VI  - 407
IP  - 20
DP  - 2015 Aug
TI  - High-internal-phase-emulsion polymeric monolith coupled with liquid 
      chromatography-electrospray tandem mass spectrometry for enrichment and sensitive 
      detection of trace cytokinins in plant samples.
PG  - 6071-9
LID - 10.1007/s00216-015-8782-3 [doi]
AB  - High-internal-phase-emulsion polymers (polyHIPEs) show great promise as 
      solid-phase-extraction (SPE) materials because of the tremendous porosity and 
      highly interconnected framework afforded by the high-internal-phase-emulsion 
      (HIPE) technique. In this work, polyHIPE monolithic columns as novel SPE 
      materials were prepared and applied to trace enrichment of cytokinins (CKs) from 
      complex plant samples. The polyHIPE monoliths were synthesized via the in-situ 
      polymerization of the continuous phase of a HIPE containing styrene (STY) and 
      divinylbenzene (DVB) in a stainless column, and revealed highly efficient and 
      selective enrichment ability for aromatic compounds. Under the optimized 
      experimental conditions, a method using a monolithic polyHIPE column combined 
      with liquid chromatography-electrospray tandem mass spectrometry (LC-MS-MS) was 
      developed for the simultaneous extraction and sensitive determination of 
      trans-zeatin (tZ), meta-topolin (mT), kinetin (K), and kinetin riboside (KR). The 
      proposed method had good linearity, with correlation coefficients (R (2)) from 
      0.9957 to 0.9984, and low detection limits (LODs, S/N = 3) in the range 
      2.4-47 pg mL(-1) for the four CKs. The method was successfully applied to the 
      determination of CKs in real plant samples, and obtained good recoveries ranging 
      from 68.8 % to 103.0 % and relative standard deviations (RSDs) lower than 16 %.
FAU - Du, Fuyou
AU  - Du F
AD  - Guangxi Key Laboratory of Electrochemical and Magnetochemical Functional 
      Materials, College of Chemistry and Bioengineering, Guilin University of 
      Technology, Guangxi, 541004, China, dufu2005@126.com.
FAU - Sun, Lin
AU  - Sun L
FAU - Zhen, Xian
AU  - Zhen X
FAU - Nie, Honggang
AU  - Nie H
FAU - Zheng, Yanjie
AU  - Zheng Y
FAU - Ruan, Guihua
AU  - Ruan G
FAU - Li, Jianping
AU  - Li J
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150530
PL  - Germany
TA  - Anal Bioanal Chem
JT  - Analytical and bioanalytical chemistry
JID - 101134327
RN  - 0 (Cytokinins)
RN  - 0 (Emulsions)
RN  - 0 (Polymers)
RN  - 0 (Styrenes)
RN  - 0 (Vinyl Compounds)
RN  - 0 (polyHIPE)
RN  - IZ715T4SBU (divinyl benzene)
SB  - IM
MH  - Chromatography, High Pressure Liquid/*methods
MH  - Cytokinins/*analysis/isolation & purification
MH  - Emulsions/chemistry
MH  - Fabaceae/*chemistry
MH  - Limit of Detection
MH  - Plant Leaves/chemistry
MH  - Polymers/chemistry
MH  - Solid Phase Extraction/*methods
MH  - Spectrometry, Mass, Electrospray Ionization/*methods
MH  - Styrenes/chemistry
MH  - Tandem Mass Spectrometry/methods
MH  - Nicotiana/*chemistry
MH  - Vinyl Compounds/chemistry
EDAT- 2015/05/31 06:00
MHDA- 2016/04/22 06:00
CRDT- 2015/05/31 06:00
PHST- 2015/01/06 00:00 [received]
PHST- 2015/05/11 00:00 [accepted]
PHST- 2015/05/09 00:00 [revised]
PHST- 2015/05/31 06:00 [entrez]
PHST- 2015/05/31 06:00 [pubmed]
PHST- 2016/04/22 06:00 [medline]
AID - 10.1007/s00216-015-8782-3 [doi]
PST - ppublish
SO  - Anal Bioanal Chem. 2015 Aug;407(20):6071-9. doi: 10.1007/s00216-015-8782-3. Epub 
      2015 May 30.