PMID- 26026464
OWN - NLM
STAT- MEDLINE
DCOM- 20160822
LR  - 20181113
IS  - 1573-2576 (Electronic)
IS  - 0360-3997 (Linking)
VI  - 38
IP  - 6
DP  - 2015 Dec
TI  - Chitotriosidase Expression during Monocyte-Derived Dendritic Cells 
      Differentiation and Maturation.
PG  - 2082-91
LID - 10.1007/s10753-015-0190-5 [doi]
AB  - The chitotriosidase (CHIT-1) is a glycosyl hydrolase (GH), which has been found 
      highly expressed in activated macrophages and in different monocyte-derived cell 
      lines such as Kupffer cells and osteoclasts, as well is differently produced in 
      diverse stages of macrophage polarization (M1 and M2). Recent finding suggests 
      that CHIT-1 plays a crucial role in innate and acquired immunity. Dendritic cells 
      (DCs) are a complex group of cells that play a critical role in immune response. 
      The aim of this study was to investigate the presence of CHIT-1 during the 
      differentiation and maturation of DCs. Magnetically-isolated peripheral blood 
      monocytes were differentiated toward immature DCs (iDC) and mature DCs (mDCs). 
      Our results showed, for the first time, that CHIT-1 is expressed during the 
      process of differentiation and maturation of DCs in a time-dependent manner. We 
      found that CHIT1 is evenly distributed in cytoplasm of both the iDCs and mDCs. 
      Additionally, a significantly increased expression of CHIT1 mRNA and protein was 
      observed in mature DCs. These results suggest that CHIT-1 play an important role 
      in the DCs immunoresponse.
FAU - Di Rosa, Michelino
AU  - Di Rosa M
AD  - Department of Bio-medical and Biotechnological Sciences, University of Catania, 
      Catania, Italy. mdirosa@unict.it.
FAU - Tibullo, Daniele
AU  - Tibullo D
AD  - Department of Clinical and Molecular Biomedicine, University of Catania, Ospedale 
      Ferrarotto, Italy.
FAU - Cambria, Daniela
AU  - Cambria D
AD  - Department of Bio-medical and Biotechnological Sciences, University of Catania, 
      Catania, Italy.
FAU - Distefano, Gisella
AU  - Distefano G
AD  - Department of Bio-medical and Biotechnological Sciences, University of Catania, 
      Catania, Italy.
FAU - Saccone, Salvatore
AU  - Saccone S
AD  - Department of Biological, Geological and Environmental Sciences, University of 
      Catania, Catania, Italy.
FAU - Di Raimondo, Francesco
AU  - Di Raimondo F
AD  - Department of Clinical and Molecular Biomedicine, University of Catania, Ospedale 
      Ferrarotto, Italy.
FAU - Malaguarnera, Lucia
AU  - Malaguarnera L
AD  - Department of Bio-medical and Biotechnological Sciences, University of Catania, 
      Catania, Italy. lucmal@unict.it.
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - United States
TA  - Inflammation
JT  - Inflammation
JID - 7600105
RN  - 0 (RNA, Messenger)
RN  - EC 3.2.1.- (Hexosaminidases)
RN  - EC 3.2.1.- (chitotriosidase)
SB  - IM
MH  - *Cell Differentiation
MH  - Cells, Cultured
MH  - Cytoplasm/enzymology
MH  - Dendritic Cells/*enzymology/immunology
MH  - Gene Expression Regulation, Enzymologic
MH  - Hexosaminidases/genetics/*metabolism
MH  - Humans
MH  - Monocytes/*enzymology/immunology
MH  - Phenotype
MH  - RNA, Messenger/metabolism
MH  - Signal Transduction
MH  - Time Factors
OTO - NOTNLM
OT  - chitotriosidase
OT  - immunity
OT  - monocyte derived dendritic cells
EDAT- 2015/06/01 06:00
MHDA- 2016/08/23 06:00
CRDT- 2015/06/01 06:00
PHST- 2015/06/01 06:00 [entrez]
PHST- 2015/06/01 06:00 [pubmed]
PHST- 2016/08/23 06:00 [medline]
AID - 10.1007/s10753-015-0190-5 [pii]
AID - 10.1007/s10753-015-0190-5 [doi]
PST - ppublish
SO  - Inflammation. 2015 Dec;38(6):2082-91. doi: 10.1007/s10753-015-0190-5.