PMID- 26026617
OWN - NLM
STAT- MEDLINE
DCOM- 20160531
LR  - 20181113
IS  - 1573-4919 (Electronic)
IS  - 0300-8177 (Linking)
VI  - 407
IP  - 1-2
DP  - 2015 Sep
TI  - Response of endothelial cells and pericytes to hypoxia and erythropoietin in a 
      co-culture assay dedicated to soft tissue repair.
PG  - 29-40
LID - 10.1007/s11010-015-2451-x [doi]
AB  - The increasing mean life expectancy of the citizens of the western world 
      countries leads to an increase of the age-related diseases, among them soft 
      tissue defects exhibiting inadequate healing. In order to develop new therapeutic 
      strategies to support disturbed soft tissue repair, there is a strong need of 
      sophisticated in vitro assays. A new assay combining scratch wounding with 
      co-cultures of primary human microvascular endothelial cells (HDMEC) and 
      pericytes (HPC) focuses on basic characteristics of cell interaction against the 
      background of soft tissue repair. The cell parameters proliferation, migration 
      and differentiation, and the release of monocyte chemoattractant protein-1 
      (MCP-1) were analysed in response to hypoxia (pO2 < 5 mmHg) and to erythropoietin 
      (EPO; 50 IU/ml), a glycoprotein hormone having shown promising effects in soft 
      tissue repair. As basic characteristics of the assay, direct cell contact in 
      co-culture led to a weakened proliferation of both cell types, an increase of the 
      percentage of myofibroblast-like pericytes and to a higher release of MCP-1. 
      Hypoxia caused a proliferation decrease of HPC in co-culture, which was slightly 
      attenuated by EPO. Hypoxia also reduced the MCP-1 release of co-cultured cells, 
      when EPO had been added. In addition, EPO had a rather positive effect on HPC 
      migration under hypoxia. These in vitro results allow new insights into the 
      interaction of pericytes with endothelial cells in the context of soft tissue 
      repair.
FAU - Schneider, Gerrit
AU  - Schneider G
AD  - Department of Trauma-, Hand- and Reconstructive Surgery, Saarland University, 
      Kirrberger Strasse, Bldng. 57, 66421, Homburg, Germany.
FAU - Bubel, Monika
AU  - Bubel M
FAU - Pohlemann, Tim
AU  - Pohlemann T
FAU - Oberringer, Martin
AU  - Oberringer M
LA  - eng
PT  - Journal Article
DEP - 20150531
PL  - Netherlands
TA  - Mol Cell Biochem
JT  - Molecular and cellular biochemistry
JID - 0364456
RN  - 0 (CCL2 protein, human)
RN  - 0 (Chemokine CCL2)
RN  - 11096-26-7 (Erythropoietin)
SB  - IM
MH  - Cell Differentiation/drug effects
MH  - Cell Hypoxia
MH  - Cell Line
MH  - Cell Movement/drug effects
MH  - Cell Proliferation/drug effects
MH  - Chemokine CCL2/*metabolism
MH  - Coculture Techniques/*methods
MH  - Endothelial Cells/*cytology/metabolism
MH  - Erythropoietin/*pharmacology
MH  - Humans
MH  - Pericytes/*cytology/metabolism
MH  - Wound Healing
EDAT- 2015/06/01 06:00
MHDA- 2016/06/01 06:00
CRDT- 2015/06/01 06:00
PHST- 2015/03/11 00:00 [received]
PHST- 2015/05/07 00:00 [accepted]
PHST- 2015/06/01 06:00 [entrez]
PHST- 2015/06/01 06:00 [pubmed]
PHST- 2016/06/01 06:00 [medline]
AID - 10.1007/s11010-015-2451-x [doi]
PST - ppublish
SO  - Mol Cell Biochem. 2015 Sep;407(1-2):29-40. doi: 10.1007/s11010-015-2451-x. Epub 
      2015 May 31.